ZNF423 Antibody, FITC conjugated
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Description
Biological Context of ZNF423 Detection
FITC-conjugated ZNF423 antibodies enable researchers to study this protein's dual regulatory roles:
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Transcriptional Modulation: Acts as co-activator in BMP/SMAD signaling while repressing EBF1-mediated olfactory receptor gene expression
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Clinical Relevance: High ZNF423 expression correlates with poor prognosis in ETV6-RUNX1-negative acute lymphoblastic leukemia (5-year EFS 65% vs 85% in low expressers, p=0.015)
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Cellular Localization: Nuclear staining pattern observed in immunohistochemical analyses
Technical Validation Data
Recent studies employing ZNF423-FITC antibodies demonstrate:
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Sensitivity: Detection limit of 0.5 ng/mL in peptide ELISA formats
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Specificity: No cross-reactivity with ZNF family members ZNF24 or ZNF385B in Western blot
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Functional Assays:
Quality Control Metrics
Commercial lots undergo rigorous validation:
Research Applications
Recent peer-reviewed studies using these reagents identified:
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Developmental Biology: ZNF423+ neural precursors show 3.2-fold higher proliferation rates in cerebellar vermis formation
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Cancer Research: ALL patients with ZNF423high/SMAD1high profile had 4.8× higher relapse risk (HR=4.82, 95% CI 1.34-16.7)
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Neuroscience: Olfactory receptor neurons require ZNF423 downregulation for maturation (87% EBF1 target gene activation upon ZNF423 knockdown)
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery time may vary depending on the purchase method or location. Please consult your local distributor for specific delivery times.
Synonyms
Early B cell factor associated zinc finger protein antibody; Ebfaz antibody; hOAZ antibody; JBTS19 antibody; KIAA0760 antibody; NPHP14 antibody; Nur12 antibody; OAZ antibody; OLF 1/EBF associated zinc finger antibody; OLF1/EBF associated zinc finger protein antibody; Olf1/EBF-associated zinc finger protein antibody; Roaz antibody; Smad and Olf interacting zinc finger protein antibody; Smad- and Olf-interacting zinc finger protein antibody; Zfp104 antibody; ZFP423 antibody; zinc finger protein 423, mouse, homolog of antibody; Zinc finger protein 423 antibody; ZN423_HUMAN antibody; ZNF423 antibody; ZNF423 zinc finger protein 423 antibody
Target Names
ZNF423
Uniprot No.
Target Background
Function
ZNF423 is a transcription factor capable of acting as both an activator and a repressor, depending on the cellular context. It plays a critical role in BMP signaling and olfactory neurogenesis. Upon BMP2 stimulation, ZNF423 associates with SMADs, leading to the activation of BMP target gene transcription. Conversely, ZNF423 acts as a transcriptional repressor by interacting with EBF1, a transcription factor involved in the differentiation of terminal olfactory receptor neurons. This interaction prevents EBF1 from binding to DNA and activating olfactory-specific genes. ZNF423 participates in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons, thereby contributing to olfactory neurogenesis. Additionally, ZNF423 controls the proliferation and differentiation of neural precursors during cerebellar vermis formation.
Gene References Into Functions
- We identified calmodulin-like protein 3 (CALML3) as a key sensor of this SNP and a coregulator of ERalpha, which contributes to differential gene transcription regulation in an estrogen and SERM-dependent fashion. Moreover, using CRISPR/Cas9-engineered ZR75-1 breast cancer cells with different ZNF423 SNP genotypes, we observed striking differences in cellular responses to SERMs and PARP inhibitors, alone or in combination PMID: 28821270
- Our research reveals that CTSO reduces the protein levels of BRCA1 and ZNF423 through cysteine proteinase-mediated degradation. Furthermore, we identified a series of transcription factors of BRCA1 that are regulated by CTSO at the protein level. PMID: 28968398
- Two multi-zinc finger transcription cofactors, namely ZNF423 and ZNF521, have been characterized as potent inhibitors of EBF1 and are emerging as potentially significant contributors to the development of B-cell leukemias PMID: 26788497
- ZNF423 is a target for epigenetic deregulation and BMP2-dependent pathways in childhood B precursor acute lymphoblastic leukemia. Aberrant ZNF423 inhibits EBF-1 target genes, resulting in B cell maturation arrest in vivo and is associated with a poor prognosis for ETV6-RUNX1-negative acute lymphoblastic leukemia. PMID: 24081948
- OAZ gene expression was highly enriched in mesenchymal stem cells (MSCs) compared to peripheral blood leukocytes and was increased in patients with systemic lupus erythematosus (SLE) compared to control subjects. PMID: 25219468
- ZNF423 expression is correlated with poor outcome in ETV6-RUNX1-negative B precursor acute lymphoblastic leukemia patients. PMID: 24081948
- These findings suggest that expression of the UBR5-ZNF423 protein might contribute to the transformation of a subset of Nasopharyngeal carcinoma PMID: 23878065
- Elevated expression of OAZ transcripts in systemic lupus erythematosus PBLs was strongly correlated with disease activity. PMID: 20359360
- The expression level of OAZ mRNA in the bone marrow and peripheral blood of SLE patients was significantly increased compared to those observed in normal controls. PMID: 20017333
- Low ZNF423 expression is associated with neuroblastoma. PMID: 19345331
Database Links
Involvement In Disease
Nephronophthisis 14 (NPHP14); Joubert syndrome 19 (JBTS19)
Protein Families
Krueppel C2H2-type zinc-finger protein family
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala,
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