ZNF446 Antibody
Description
Overview of ZNF446 Antibody
The ZNF446 antibody is a polyclonal or monoclonal immunoglobulin designed to target the zinc finger protein 446 (ZNF446), a transcriptional regulator involved in cellular processes such as DNA binding and chromatin remodeling. Its primary applications include Western blot (WB), immunofluorescence (IF/ICC), and ELISA assays.
Key Specifications (Polyclonal Antibody 16218-1-AP) :
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Host/Isotype: Rabbit IgG
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Reactivity: Human
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Immunogen: ZNF446 fusion protein (Ag9266)
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Molecular Weight: Observed 49–55 kDa (calculated 46 kDa)
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Storage: PBS with 0.02% sodium azide and 50% glycerol, stored at -20°C
Applications and Performance
The antibody demonstrates robust reactivity in human samples across multiple techniques:
| Application | Dilution Range | Detection |
|---|---|---|
| Western Blot (WB) | 1:200–1:1000 | SH-SY5Y cells |
| Immunofluorescence (IF/ICC) | 1:50–1:500 | SH-SY5Y cells |
| ELISA | N/A | N/A |
Published Validation:
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Co-occupancy with SMAD3 at promoter regions in triple-negative breast cancer (TNBC) cells, linked to TGFβ signaling regulation .
Biological Significance in Research
ZNF446 interacts with transcriptional complexes to modulate gene expression:
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TGFβ Signaling in TNBC:
ZNF446 co-occupies SMAD3 binding sites, influencing oncogenic pathways. Its depletion reduces anchorage-independent growth in TNBC cells . -
KRAB Zinc Finger Protein Network:
Part of a broader interactome involving KRAB-associated zinc finger proteins (KZFPs), which regulate genomic imprinting, RNA metabolism, and retrotransposon silencing .
Product Comparison
Two notable antibody variants are available:
| Product | Type | Host | Reactivity | Applications |
|---|---|---|---|---|
| 16218-1-AP (Proteintech) | Polyclonal | Rabbit | Human | WB, IF/ICC, ELISA |
| PCRP-ZNF446-1B4 (DSHB) | Monoclonal | Mouse | Human | Unspecified |
Key Differences:
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The polyclonal antibody offers broader epitope recognition, while the monoclonal variant (Mouse IgG2b) provides higher specificity .
Research Implications
ZNF446 antibodies are critical tools for studying transcriptional regulation and cancer biology. Their utility extends to:
Citations: Proteintech. (2025). ZNF446 Polyclonal Antibody (16218-1-AP). Retrieved from *https://www.ptglab.com* BioRxiv. (2020). The testis protein ZNF165 is a SMAD3 cofactor in TNBC. doi:10.1101/2020.04.04.025742 EMBO Press. (2019). Interactome of KRAB zinc finger proteins. doi:10.15252/embj.2018101220 DSHB. (2013). PCRP-ZNF446-1B4. Retrieved from https://dshb.biology.uiowa.edu
Product Specs
Target Background
- Studies in COS-7 cells demonstrate that ZNF446 overexpression inhibits the transcriptional activity of both SRE and AP-1. PMID: 15936718
