ZNF644 Antibody, FITC conjugated
Description
Product Overview
ZNF644 Antibody, FITC conjugated is a rabbit polyclonal antibody covalently linked to fluorescein isothiocyanate (FITC), enabling visualization of ZNF644 in cellular and molecular studies. Key features include:
| Parameter | Details |
|---|---|
| Target | ZNF644 (UniProt ID: Q9H582) |
| Host Species | Rabbit |
| Conjugate | FITC (Excitation/Emission: 499/515 nm) |
| Immunogen | Recombinant Human ZNF644 protein (residues 752-913) |
| Applications | Western blot (WB), ELISA, Immunoprecipitation (IP) |
| Reactivity | Human, predicted for mouse and rat due to high homology |
| Purification | Protein G affinity chromatography (>95% purity) |
| Storage | -20°C or -80°C in 50% glycerol, 0.01M PBS (pH 7.4) |
| Research Areas | Epigenetics, transcriptional regulation, autoimmune diseases |
Immunogen and Specificity
The antibody is generated against a synthetic peptide spanning amino acids 752–913 of human ZNF644, a region critical for its zinc finger domains . Specificity is validated via:
-
Western blot: Detects a single band at ~150 kDa in human cell lysates (HeLa, 293T) .
-
Immunoprecipitation: Confirms interaction with endogenous ZNF644 in 293T cells .
3.1. Epigenetic Regulation
ZNF644 interacts with histone methyltransferases G9a/GLP to mediate H3K9 methylation, a hallmark of gene silencing. The FITC-conjugated antibody has been used to study this interaction in chromatin remodeling assays .
3.2. Autoimmune Disease Mechanisms
Elevated ZNF644 expression correlates with Graves' disease (GD) progression. The antibody aids in detecting ZNF644 in peripheral blood mononuclear cells (PBMCs), where it regulates circZNF644/miR-29a-3p/ICOS signaling in follicular helper T cells .
3.3. Subcellular Localization
FITC conjugation enables fluorescence microscopy to confirm ZNF644’s cytoplasmic and nuclear localization, critical for its role as a ceRNA (competing endogenous RNA) .
Research Findings
Validation and Quality Control
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Our research suggests that genetic variations in GRM6 are associated with high myopia. Further investigation is needed to understand the precise role of GRM6 in the development of high myopia. PMID: 27034204
- ZNF644 has been linked to high myopia in a study conducted on a Chinese population cohort. PMID: 24991186
- Two novel variants in ZNF644 have been associated with high-grade myopia in a US cohort. PMID: 22539872
- ZNF644 might be a causal gene for high myopia in a monogenic form. PMID: 21695231
