ANGPTL2 Human
Description
Physiological Roles in Tissue Homeostasis
ANGPTL2 maintains tissue integrity through:
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Adaptive Inflammation: Recruits immune cells to repair damaged tissues while preventing excessive inflammatory damage .
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Angiogenesis: Promotes endothelial cell survival and vascular network formation, particularly under hypoxic conditions .
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Hematopoiesis: Enhances hematopoietic stem/progenitor cell (HSPC) survival via NOTCH activation, mediated by receptor LILRB2 .
Key Physiological Functions:
| Process | Mechanism | Outcome |
|---|---|---|
| Tissue repair | Recruits macrophages and promotes extracellular matrix remodeling | Restores tissue integrity |
| Metabolic regulation | Modulates lipoprotein lipase activity and adipose tissue lipid storage | Maintains energy balance |
| Cardiovascular health | Supports endothelial barrier function and prevents vascular leakage | Preserves vascular integrity |
Pathophysiological Roles in Disease
ANGPTL2 overexpression is linked to chronic inflammatory and metabolic disorders:
Metabolic Diseases
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Obesity and Insulin Resistance: Elevated ANGPTL2 in adipose tissue correlates with adipose tissue inflammation, impaired adipogenesis, and insulin resistance .
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Diabetes: Adipose-derived ANGPTL2 promotes macrophage infiltration and pro-inflammatory cytokine secretion (e.g., TNF-α, IL-6), exacerbating systemic inflammation .
Cardiovascular Diseases
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Atherosclerosis: Drives endothelial dysfunction, smooth muscle cell proliferation, and plaque formation .
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Heart Failure: Overexpression in pathologically remodeled hearts accelerates dysfunction by inhibiting AKT-SERCA2a signaling and reducing ATP production .
Cancer
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Tumor Progression: Acts as a dual regulator, suppressing metastasis in ovarian cancer but promoting it in other cancers (e.g., colorectal, breast) via angiogenesis and immune evasion .
Research Findings: Key Studies and Mechanisms
Therapeutic Potential
ANGPTL2 represents a promising therapeutic target for metabolic and cardiovascular diseases. Strategies include:
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Inhibition: Small interfering RNA (siRNA) or monoclonal antibodies to block ANGPTL2-LILRB2 interactions .
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Recombinant Protein: Preclinical studies use human recombinant ANGPTL2 to study angiogenesis and metabolic regulation .
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Biomarker: Elevated serum ANGPTL2 levels correlate with chronic inflammation and disease severity, aiding diagnostic applications .
Product Specs
Recombinant Human ANGPTL2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 493 amino acids (22-493 a.a.). This includes a 20 a.a. N-terminal His tag, resulting in a total molecular mass of 57.1kDa (calculated).
The product appears as a clear, colorless solution that has been sterilized by filtration.
The ANGPTL2 protein solution is provided at a concentration of 0.5mg/ml and is buffered in a solution of 20mM Tris HCL (pH 7-8) with 10% glycerol.
For short-term storage (2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It is important to minimize repeated freeze-thaw cycles to preserve product integrity.
The purity of the ANGPTL2 protein is determined to be greater than 85.0% based on SDS-PAGE analysis.
MGSSHHHHHH SSGLVPRGSH MGQEDGFEGT EEGSPREFIY LNRYKRAGES QDKCTYTFIV PQQRVTGAIC VNSKEPEVLL ENRVHKQELE LLNNELLKQK RQIETLQQLV EVDGGIVSEV KLLRKESRNM NSRVTQLYMQ LLHEIIRKRD NALELSQLEN RILNQTADML QLASKYKDLE HKYQHLATLA HNQSEIIAQL EEHCQRVPSA RPVPQPPPAA PPRVYQPPTY NRIINQISTN EIQSDQNLKV LPPPLPTMPT LTSLPSSTDK PSGPWRDCLQ ALEDGHDTSS IYLVKPENTN RLMQVWCDQR HDPGGWTVIQ RRLDGSVNFF RNWETYKQGF GNIDGEYWLG LENIYWLTNQ GNYKLLVTME DWSGRKVFAE YASFRLEPES EYYKLRLGRY HGNAGDSFTW HNGKQFTTLD RDHDVYTGNC AHYQKGGWWY NACAHSNLNG VWYRGGHYRS RYQDGVYWAE FRGGSYSLKK VVMMIRPNPN TFH
Product Science Overview
ANGPTL2 is a secreted glycoprotein that does not bind to the Tie-1 or Tie-2 receptors, which are typically associated with angiopoietins . The mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs . It is widely expressed in many tissues and its expression is induced by chronic, but not acute, hypoxia .
ANGPTL2 has been shown to induce sprouting in endothelial cells, although it is not mitogenic . It plays a significant role in promoting the expansion of E16 rat liver mononuclear cells in vitro, especially in the presence of other growth factors such as Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand . The protein is also involved in various inflammatory processes and has been linked to metabolic regulation.
Recombinant human ANGPTL2 is typically produced using a mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 2/ANGPTL2 protein . The protein is purified to a high degree, with a purity greater than 75% as determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining . The endotoxin level is kept below 0.10 EU per 1 μg of the protein by the LAL method .
The recombinant protein is typically lyophilized from a 0.2 μm filtered solution and can be reconstituted at 200 μg/mL in water . It is shipped at ambient temperature and should be stored at -20 to -70 °C to maintain its stability. Once reconstituted, it can be stored under sterile conditions at 2 to 8 °C for up to one month or at -20 to -70 °C for up to three months .
