Product List

ANGPT1 Human

Angiopoietin-1 Human Recombinant

ANGPT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 480 amino acids (20-498) and having a molecular mass of 55.6 kDa.
The ANGPT1 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT9019
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

ANGPT2 Human

Angiopoietin 2 Human Recombinant

ANGPT2 produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain (19-496 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 55.7kDa.
ANGPT2 shows multiple bands between 50-100kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT9125
Source

Chinese Hamster Ovary Cells (CHO).

Appearance

Sterile filtered colorless solution.

ANGPTL2 Human

Angiopoietin-like Protein 2 Human Recombinant

ANGPTL2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 493 amino acids ( 22-493 a.a.) including a 20 a.a N-terminal His tag. The total molecular mass is 57.1kDa (calculated).

Shipped with Ice Packs
Cat. No.
BT9233
Source
Escherichia Coli.
Appearance

Sterile filtered colorless solution.

ANGPTL3 (17-460) Human

Angiopoietin-like Protein 3 (17-460 a.a.) Human Recombinant

ANGPTL3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 453 amino acids (17-460 a.a.) and having a molecular mass of 52.9kDa (Migrates at 25-70kDa on SDS-PAGE under reducing conditions). 
ANGPTL3 is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT9322
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

ANGPTL3 (243-460) Human

Angiopoietin-like Protein 3 (243-460 a.a.) Human Recombinant

ANGPTL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (243-460 a.a) and having a molecular mass of 27.7kDa.
ANGPTL3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT9785
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

ANGPTL3 Human

Angiopoietin Like Protein 3 Human Recombinant

The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag. The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human (26-233 a.a.) and 16 additional amino acid residues – His-Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT9862
Source
Escherichia Coli.
Appearance

ANGPTL3 Human, HEK

Angiopoietin Like Protein 3 Human Recombinant, HEK

ANGPTL3 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 17-460) containing a total of 450 amino acids, having a molecular mass of 52.6kDa (calculated) and fused to a 6 aa His tag at C-Terminus.
The Human ANGPTL3 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT9946
Source
HEK 293.
Appearance
Filtered White lyophilized (freeze-dried) powder.

ANGPTL4 Human

Angiopoietin-like Protein 4 Human Recombinant

The ANGPTL4 Human Recombinant is manufactured with N-terminal fusion of His Tag.
The Angiopoietin-like Protein 4 His -Tagged Fusion Protein is 25 kDa protein containing 204 amino acid residues of the Angiopoietin-like Protein 4 and 16 additional amino acid residues - His Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT10038
Source
Escherichia Coli.
Appearance
Filtered White lyophilized (freeze-dried) powder.

ANGPTL4 Human, HEK

Angiopoietin-like Protein 4 Human Recombinant, HEK

The ANGPTL4 Human Recombinant is manufactured with C-terminal fusion of 11 amino acid FLAG Tag.
The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues - Flag Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT10474
Source
HEK293.
Appearance
Filtered White lyophilized (freeze-dried) powder.

ANGPTL7 Human

Angiopoietin-like Protein 7 Human Recombinant

ANGPTL7 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (27-346a.a) containing 553amino acids and having a molecular mass of 63.2kDa.
ANGPTL7 is fused to a 233 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT10609
Source

HEK293 cells.

Appearance

Sterile filtered colorless solution.

Introduction

Definition and Classification

Angiopoietins are a family of secreted glycoproteins that play a crucial role in vascular development and angiogenesis. They are primarily involved in the maturation, stability, and remodeling of blood vessels . The angiopoietin family consists of four main members: Angiopoietin-1 (Ang1), Angiopoietin-2 (Ang2), Angiopoietin-3 (Ang3), and Angiopoietin-4 (Ang4) . These proteins act as ligands for the Tie2 receptor, a receptor tyrosine kinase predominantly expressed on endothelial cells .

Biological Properties

Key Biological Properties: Angiopoietins are characterized by their ability to regulate blood vessel formation and maintenance. Ang1 is known for promoting vessel maturation and stability, while Ang2 acts as an antagonist to Ang1, promoting vascular remodeling and destabilization .

Expression Patterns and Tissue Distribution: Ang1 is widely expressed in various tissues, including the heart, lungs, and kidneys, and is secreted by pericytes and smooth muscle cells . Ang2 is primarily expressed in the endothelium and is stored in Weibel-Palade bodies, allowing for rapid release in response to stimuli . Ang3 and Ang4 have more restricted expression patterns and are less well-characterized .

Biological Functions

Primary Biological Functions: Angiopoietins are essential for angiogenesis, the process of forming new blood vessels from pre-existing ones. Ang1 promotes endothelial cell survival, migration, and adhesion, contributing to vessel maturation and stability . Ang2, on the other hand, facilitates vascular remodeling by promoting endothelial cell apoptosis and loosening cell-cell junctions .

Role in Immune Responses and Pathogen Recognition: Angiopoietins also play a role in immune responses by regulating the permeability of blood vessels, which can influence the migration of immune cells to sites of infection or inflammation . Ang2, in particular, has been implicated in promoting inflammation by increasing vascular permeability .

Modes of Action

Mechanisms with Other Molecules and Cells: Angiopoietins interact with the Tie2 receptor to mediate their effects. Ang1 binding to Tie2 leads to receptor dimerization and autophosphorylation, activating downstream signaling pathways that promote cell survival and vessel stability . Ang2 can act as a competitive inhibitor of Ang1, preventing Tie2 activation and promoting vascular remodeling .

Binding Partners and Downstream Signaling Cascades: The primary binding partner for angiopoietins is the Tie2 receptor. Upon binding, Ang1 activates several downstream signaling cascades, including the PI3K/Akt and MAPK pathways, which are involved in cell survival, proliferation, and migration . Ang2, by inhibiting Tie2 activation, can disrupt these signaling pathways and promote vascular destabilization .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of angiopoietins is tightly regulated at both the transcriptional and post-transcriptional levels. Hypoxia and inflammatory cytokines can upregulate Ang2 expression, while Ang1 expression is more constitutive . Post-translational modifications, such as phosphorylation, also play a role in modulating the activity of angiopoietins .

Transcriptional Regulation and Post-Translational Modifications: Transcription factors such as HIF-1α (hypoxia-inducible factor 1-alpha) can induce the expression of Ang2 under hypoxic conditions . Additionally, proteolytic cleavage and glycosylation are post-translational modifications that can affect the stability and activity of angiopoietins .

Applications

Biomedical Research: Angiopoietins are extensively studied in the context of cancer, where they are involved in tumor angiogenesis and metastasis. Targeting angiopoietin signaling pathways is a promising strategy for developing anti-angiogenic therapies .

Diagnostic Tools: Elevated levels of Ang2 have been associated with various pathological conditions, including cancer and inflammatory diseases, making it a potential biomarker for diagnosis and prognosis .

Therapeutic Strategies: Therapeutic approaches targeting angiopoietin signaling, such as Ang2 inhibitors, are being developed to treat diseases characterized by abnormal angiogenesis, including cancer and diabetic retinopathy .

Role in the Life Cycle

Role Throughout the Life Cycle: Angiopoietins play a critical role throughout the life cycle, from embryonic development to aging. During embryogenesis, they are essential for the formation and maturation of the vascular system . In adults, they maintain vascular homeostasis and are involved in tissue repair and regeneration . Dysregulation of angiopoietin signaling is associated with various age-related diseases, including cardiovascular diseases and cancer .

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