DHH (C23II) His Human

Desert HedgeHog (C23II) Human Recombinant, His Tag

Recombinant Human DHH (C23II) His, produced in E.coli, is a single, non-glycosylated polypeptide chain comprising 201 amino acids (23-198) with a molecular weight of 22.4kDa. This protein features a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23780
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

DHH (C23II) Human

Desert Hedgehog (C23II) Human Recombinant

Recombinant human DHH (C23II) is produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 177 amino acids with a molecular weight of 19.9 kDa. The purification of DHH (C23II) is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23890
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.

SHH (C24II) Human

Sonic HedgeHog (C24II) Human Recombinant

Recombinant human SHH (C24II) is produced in E. coli. This non-glycosylated polypeptide chain contains 176 amino acids (24-197 a.a.), with a molecular weight of 19.8 kDa. The cysteine at position 24 is replaced with two isoleucines. SHH is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24317
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

SHH Human

Sonic HedgeHog Human Recombinant

Sonic HedgeHog Recombinant Human, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 179 amino acids. It has a molecular weight of 20.2kDa. The cysteine residue at position 2 has been replaced with two isoleucine residues.
Shipped with Ice Packs
Cat. No.
BT24390
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder

SHH Human, His

Sonic HedgeHog Human Recombinant, His Tag

Recombinant Human Sonic Hedgehog, expressed in E. coli, is a non-glycosylated polypeptide chain containing 183 amino acids with a molecular weight of 20.7 kDa. It features an 8-amino acid His-tag fused at the C-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24450
Source
Escherichia Coli.
Appearance
A clear and colorless solution, sterile-filtered.

SHH Mouse

Sonic Hedgehog Mouse Recombinant

Recombinant Mouse Sonic Hedgehog, produced in E. coli, is an unglycosylated polypeptide chain composed of 176 amino acids, with a molecular weight of 19.8 kDa. It shares 99% homology with the human Sonic Hedgehog gene. The cysteine residue at position 25 has been replaced with isoleucine. Purification of Sonic Hedgehog is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT24517
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

DHH (C23II) Mouse

Desert Hedgehog (C23II) Mouse Recombinant

Recombinant Mouse DHH (C23II) is a non-glycosylated monomeric protein produced in E. coli. With a molecular weight of 20 kDa, it consists of 177 amino acids. Purification is achieved through proprietary chromatographic methods, ensuring high purity.
Shipped with Ice Packs
Cat. No.
BT23966
Source
Escherichia Coli.
Appearance
Sterile, white, lyophilized (freeze-dried) powder.

DHH Human

Desert Hedgehog Human Recombinant

Recombinant human DHH protein, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 197 amino acids (residues 23-198). This protein has a molecular weight of 22 kDa and includes an N-terminal His-tag (20 amino acids) for purification purposes. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT24041
Source
Escherichia Coli.
Appearance
The product is a clear, colorless, and sterile filtered solution.

IHH Human

Indian Hedgehog Human Recombinant

Recombinant Human IHH, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 176 amino acids with a molecular weight of 19.8 kDa. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24161
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.

IHH Mouse

Indian Hedgehog Mouse Recombinant

Recombinant Mouse IHH, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 176 amino acids, resulting in a molecular mass of 19.8 kDa. The purification process of IHH involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24247
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder.
Definition and Classification

The Hedgehog (Hh) protein family is a group of secreted signaling proteins that play crucial roles in animal development and cellular communication. The family was first discovered in the fruit fly, Drosophila melanogaster, and has since been identified in various metazoans. Hedgehog proteins are classified into three main types in mammals: Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) .

Biological Properties

Key Biological Properties: Hedgehog proteins are synthesized as precursor proteins that undergo autocatalytic cleavage to produce an active signaling molecule. The mature Hh protein is modified by the addition of cholesterol and palmitate, which are essential for its signaling activity .

Expression Patterns and Tissue Distribution: Hedgehog proteins are expressed in various tissues during embryonic development and in some adult tissues. For example, Shh is prominently expressed in the notochord, floor plate, and limb buds during development, while Ihh is expressed in the gut and cartilage .

Biological Functions

Primary Biological Functions: Hedgehog proteins are involved in the regulation of cell growth, differentiation, and tissue patterning. They play a critical role in the development of the central nervous system, limbs, and other organs .

Role in Immune Responses and Pathogen Recognition: While Hedgehog proteins are primarily known for their developmental roles, recent studies suggest they may also influence immune responses and pathogen recognition. For instance, Hh signaling has been implicated in the regulation of immune cell differentiation and function .

Modes of Action

Mechanisms with Other Molecules and Cells: Hedgehog proteins exert their effects by binding to the Patched (Ptch) receptor on target cells. This binding relieves the inhibition on the Smoothened (Smo) receptor, leading to the activation of downstream signaling pathways .

Binding Partners and Downstream Signaling Cascades: The primary binding partners of Hedgehog proteins are the Ptch receptors. Upon binding, the signaling cascade involves the activation of Gli transcription factors, which regulate the expression of target genes involved in cell proliferation, differentiation, and survival .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of Hedgehog proteins are tightly regulated at multiple levels. Transcriptional regulation involves various transcription factors and enhancers that control the expression of Hh genes .

Transcriptional Regulation and Post-Translational Modifications: Post-translational modifications, such as the addition of cholesterol and palmitate, are crucial for the proper functioning of Hedgehog proteins. These modifications facilitate the proper localization and signaling activity of the proteins .

Applications

Biomedical Research: Hedgehog proteins are extensively studied in biomedical research due to their roles in development and disease. They are used as models to understand the mechanisms of cell signaling and tissue patterning .

Diagnostic Tools and Therapeutic Strategies: Hedgehog signaling pathways are targeted in various therapeutic strategies, particularly in cancer treatment. Inhibitors of the Hedgehog pathway are being developed to treat cancers such as basal cell carcinoma and medulloblastoma .

Role in the Life Cycle

Role Throughout the Life Cycle: Hedgehog proteins play essential roles throughout the life cycle, from embryonic development to aging. During development, they are critical for the formation of various organs and tissues. In adults, they are involved in tissue maintenance and regeneration .

From Development to Aging and Disease: Dysregulation of Hedgehog signaling can lead to various developmental disorders and diseases, including congenital malformations and cancers. Understanding the role of Hedgehog proteins in these processes is crucial for developing effective therapeutic interventions .

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