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Product List
BAFF Human
B-cell Activating Factor Human Recombinant
Recombinant Human BAFF, produced in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 17.007 kDa, consisting of 153 amino acids. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs 
Cat. No.
BT13150
Source
Escherichia Coli.
Appearance
Sterile white powder obtained by lyophilization.
BAFF Human, His
B cell Activating Factor Human Recombinant, His Tag
BAFF Human Recombinant His Tag is a genetically engineered protein produced in E. coli. This recombinant protein consists of the biologically active region of human BAFF, spanning amino acids 134 to 285, fused to a His-tag at its N-terminus. The His-tag facilitates purification. This protein is non-glycosylated, meaning it lacks attached sugar molecules, and has a molecular weight of approximately 21 kDa. It is a single polypeptide chain, implying that it's a single linear chain of amino acids.
Shipped with Ice Packs
Cat. No.
BT13269
Source
Escherichia Coli.
Appearance
A clear and colorless solution that has been sterilized by filtration.
BAFF Human, Plant
BAFF (BLyS) Human Recombinant, Plant
This product consists of a single glycosylated polypeptide chain derived from the BAFF protein found in humans. It's produced using Nicotiana benthamiana plants and encompasses amino acids 134 to 285, totaling 151 amino acids. A 10-His-tag is fused to the N-terminal, resulting in a total molecular mass of 18-20kDa. Purification is achieved using standard chromatographic techniques. The chemical formula of BAFF is C830H1277N223O242S5.
Shipped with Ice Packs
Cat. No.
BT13341
Source
Nicotiana benthamiana plant
Appearance
The product appears as a sterile, white powder that has been freeze-dried.
BAFF R Human
B-cell Activating Factor Receptor Human Recombinant
Recombinant human B Lymphocyte Stimulator Receptor is produced in E. coli. This extracellular protein is a single, non-glycosylated polypeptide chain with 76 amino acids and a molecular weight of 7.7 kDa. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13391
Source
Escherichia Coli.
Appearance
The product appears as a sterile, white, lyophilized (freeze-dried) powder.
BAFFR Human, HEK
BAFF (BLyS) Receptor Human Recombinant, HEK
Recombinant human BAFFR protein is a single, glycosylated polypeptide chain encompassing amino acids 1-78. It has a total of 314 amino acids, including a C-terminal fusion to a 233 amino acid hIgG tag, resulting in a molecular mass of 34.4 kDa. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Introduction
Definition and Classification
B-Cell Activating Factor (BAFF), also known as BlyS (B Lymphocyte Stimulator), is a cytokine belonging to the tumor necrosis factor (TNF) family. It plays a crucial role in the survival, proliferation, and differentiation of B cells. BAFF is classified into three isoforms: membrane-bound BAFF, soluble BAFF, and a shorter isoform known as ΔBAFF.
Biological Properties
Key Biological Properties:
- Molecular Weight: Approximately 31 kDa.
- Structure: BAFF forms a homotrimeric structure, which is essential for its biological activity.
Expression Patterns:
- BAFF is primarily expressed by myeloid cells, including monocytes, macrophages, and dendritic cells.
- It is also produced by non-hematopoietic cells such as epithelial cells and stromal cells.
Tissue Distribution:
- BAFF is widely distributed in various tissues, including the spleen, lymph nodes, and bone marrow.
- It is also found in peripheral blood and mucosal tissues.
Biological Functions
Primary Biological Functions:
- B Cell Survival: BAFF is essential for the survival of immature and mature B cells.
- B Cell Proliferation: It promotes the proliferation of B cells in response to antigenic stimulation.
- B Cell Differentiation: BAFF supports the differentiation of B cells into plasma cells and memory B cells.
Role in Immune Responses:
- BAFF enhances the production of antibodies by B cells, thereby contributing to humoral immunity.
- It plays a role in the maintenance of B cell homeostasis and the prevention of autoimmune diseases.
Pathogen Recognition:
- BAFF indirectly influences pathogen recognition by promoting the survival and function of B cells, which are responsible for producing antibodies against pathogens.
Modes of Action
Mechanisms with Other Molecules and Cells:
- BAFF interacts with three receptors: BAFF-R (BAFF Receptor), TACI (Transmembrane Activator and CAML Interactor), and BCMA (B-Cell Maturation Antigen).
- These receptors are differentially expressed on B cells and mediate distinct signaling pathways.
Binding Partners:
- BAFF binds to its receptors on B cells, leading to receptor oligomerization and activation of downstream signaling cascades.
Downstream Signaling Cascades:
- NF-κB Pathway: BAFF-R primarily activates the canonical NF-κB pathway, promoting B cell survival and proliferation.
- PI3K/Akt Pathway: BAFF signaling also involves the PI3K/Akt pathway, which is crucial for cell survival and metabolism.
- MAPK Pathway: The MAPK pathway is activated by BAFF and contributes to B cell differentiation and function.
Regulatory Mechanisms
Regulatory Mechanisms Controlling Expression and Activity:
- Transcriptional Regulation: The expression of BAFF is regulated by various transcription factors, including NF-κB and IRF (Interferon Regulatory Factor).
- Post-Translational Modifications: BAFF undergoes post-translational modifications such as glycosylation, which are important for its stability and activity.
Applications
Biomedical Research:
- BAFF is a target for research in autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA).
- It is also studied in the context of B cell malignancies, including multiple myeloma and chronic lymphocytic leukemia (CLL).
Diagnostic Tools:
- Elevated levels of BAFF in serum can serve as a biomarker for autoimmune diseases and B cell-related disorders.
Therapeutic Strategies:
- BAFF inhibitors, such as belimumab, are used in the treatment of autoimmune diseases to reduce B cell activity and antibody production.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: BAFF is crucial for the development of B cells in the bone marrow and peripheral lymphoid organs.
- Aging: BAFF levels may change with age, influencing B cell function and susceptibility to infections and autoimmune diseases.
- Disease: Dysregulation of BAFF expression is associated with various diseases, including autoimmune disorders and B cell malignancies.
