LIF Human

Leukemia Inhibitory Factor Human Recombinant

Recombinant Human Leukemia Inhibitory Factor (LIF) is produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain consisting of 180 amino acids with a molecular weight of 19.7kDa. This LIF protein is purified using specialized chromatographic methods, resulting in a highly pure product.
Shipped with Ice Packs
Cat. No.
BT19022
Source
Escherichia Coli.
Appearance
White powder, sterilized by filtration and freeze-dried.

LIF Human, GST

Leukemia Inhibitory Factor, GST tag Human Recombinant

Produced in E. coli, our LIF is a single, non-glycosylated polypeptide chain. It consists of 415 amino acids (specifically, amino acids 23 to 202) and has a molecular weight of 47.2 kDa. For purification and detection purposes, a 236 amino acid His-GST tag is fused to the N-terminus of the LIF protein. The protein is then purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19110
Source
Escherichia Coli.
Appearance
Clear solution, sterile filtered.

LIF Human, His

Leukemia Inhibitory Factor Human Recombinant, His tag

Recombinant Human LIF, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 23 to 202 (a.a 23-202). It consists of 189 amino acids, including a 9-amino acid N-terminal His tag. The calculated molecular mass is 20.9 kDa.
Shipped with Ice Packs
Cat. No.
BT19199
Source

Escherichia Coli.

Appearance
White powder, filtered and lyophilized (freeze-dried).

LIF Human, Sf9

Leukemia Inhibitory Factor Human Recombinant, Sf9

This product contains a recombinant human LIF protein produced in Sf9 insect cells. It's a single chain of 189 amino acids with a molecular weight of 20.8 kDa. The protein has a 6 amino acid His tag for purification and is purified using chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT19290
Source
Sf9, Baculovirus cells.
Appearance
Clear and colorless solution that is sterile.

LIF Human, Yeast

LIF Human Recombinant, Yeast

Recombinant Human LIF, produced in yeast, is a single-chain polypeptide that has undergone glycosylation. It comprises 180 amino acids and has a molecular weight of 58.5 kDa. The purification process for LIF involves specialized chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19374
Source
Pichia pastoris.
Appearance
White, lyophilized (freeze-dried) powder that has been sterilized by filtration.

LIF Mouse

Leukemia Inhibitory Factor Mouse Recombinant

Recombinant Murine Leukemia Inhibitory Factor (LIF) is a single-chain protein produced in E. coli bacteria. It is not glycosylated, meaning it lacks attached sugar molecules. It comprises 181 amino acids and has a molecular weight of 20 kDa. The purification process utilizes specialized chromatographic methods to ensure its purity.
Shipped with Ice Packs
Cat. No.
BT19449
Source
Escherichia Coli.
Appearance
Sterile white powder obtained by freeze-drying.

LIF Rat

Leukemia Inhibitory Factor Rat Recombinant

This product is a laboratory-produced version of Rat Leukemia Inhibitory Factor (LIF). It is a single chain protein that is not glycosylated, meaning it lacks sugar molecules that are typically attached to proteins. It consists of 180 amino acids and has a molecular weight of 19.8 kDa. The purification process involves specialized chromatography techniques to ensure its high quality.
Shipped with Ice Packs
Cat. No.
BT19534
Source
Escherichia Coli.
Appearance
It appears as a white powder that has been freeze-dried and sterilized through filtration.

LIFR Human

Leukemia Inhibitory Factor Receptor Alpha Human Recombinant

This product is a solution of LIFR protein that has been purified from Sf9 insect cells. The protein is glycosylated, meaning that it has sugars attached to it. The molecular weight of the protein is 90.5 kDa, but it appears as a band between 100 and 150 kDa on SDS-PAGE. The protein has a 6-amino acid His tag at its C-terminus.
Shipped with Ice Packs
Cat. No.
BT19779
Source
Sf9, Baculovirus cells.
Appearance
This product is a clear, colorless liquid that has been sterilized by filtration.

LIFR Mouse

Leukemia Inhibitory Factor Receptor Alpha Mouse Recombinant

Recombinant Mouse LIFR, produced in Baculovirus, is a single, glycosylated polypeptide chain. It consists of 794 amino acids (44-828 aa), resulting in a molecular weight of 89.6kDa. The LIFR protein is engineered with a 9 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT19854
Source

Sf9, Baculovirus cells.

Appearance
A clear, sterile-filtered solution.
Definition and Classification

Leukemia Inhibitory Factor (LIF) is a cytokine belonging to the interleukin-6 (IL-6) family. It is known for its ability to inhibit the differentiation of myeloid leukemic cells, hence its name . LIF is classified as a polyfunctional glycoprotein cytokine that can affect various cell types and tissues .

Biological Properties

LIF exhibits several key biological properties:

  • Expression Patterns: LIF is expressed in various tissues, including the trophectoderm of developing embryos .
  • Tissue Distribution: It is found in multiple tissues such as bone, neural tissues, and the reproductive system .
  • Key Properties: LIF influences cell growth, differentiation, and survival. It plays roles in bone metabolism, neural development, embryogenesis, and inflammation .
Biological Functions

LIF has diverse biological functions:

  • Primary Functions: It induces the terminal differentiation of myeloid leukemic cells, preventing their proliferation . It also promotes the growth and differentiation of various target cells .
  • Role in Immune Responses: LIF is involved in immune responses by modulating the activity of macrophages and other immune cells .
  • Pathogen Recognition: It plays a role in recognizing and responding to pathogens by influencing immune cell behavior .
Modes of Action

LIF operates through several mechanisms:

  • Binding Partners: LIF binds to the LIF receptor (LIFR), which forms a heterodimer with the gp130 receptor .
  • Downstream Signaling Cascades: This binding activates multiple signaling pathways, including JAK/STAT, MAPK, and PI3K pathways . These cascades lead to changes in gene expression and cellular responses .
Regulatory Mechanisms

The expression and activity of LIF are tightly regulated:

  • Transcriptional Regulation: LIF expression is controlled at the transcriptional level by various factors .
  • Post-Translational Modifications: LIF undergoes glycosylation, which affects its stability and activity . Additionally, its signaling is modulated by proteins such as SOCS (suppressors of cytokine signaling) and PIAS (protein inhibitors of activated STAT) .
Applications

LIF has several applications in biomedical research and therapy:

  • Biomedical Research: It is used in stem cell research to maintain the pluripotency of embryonic stem cells .
  • Diagnostic Tools: LIF can serve as a biomarker for certain cancers, such as pancreatic cancer .
  • Therapeutic Strategies: LIF has potential therapeutic applications in treating infertility, cancer, and tissue regeneration .
Role in the Life Cycle

LIF plays crucial roles throughout the life cycle:

  • Development: It is essential for embryo implantation and early development .
  • Aging and Disease: LIF is involved in tissue regeneration and repair, making it relevant in aging and disease contexts . It also plays roles in neurogenesis and immune responses throughout life .
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