Acrp30 Human, Trimeric

Adiponectin Human Recombinant, Trimeric form

This product consists of the trimeric form of human adiponectin, produced in HEK293 cells. The cysteine at position 39 has been replaced with alanine (C39A), resulting in a protein that exclusively forms trimers and not hexamers or HMW forms.
Shipped with Ice Packs
Cat. No.
BT6556
Source
HEK293 (Human embryonic kidney cell line).
Appearance
White, lyophilized powder after filtration.

Acrp30 Mouse

Adiponectin Mouse Recombinant

Recombinant Mouse Acrp30 is a single, glycosylated polypeptide chain composed of 230 amino acids (spanning from amino acid positions 18 to 247a). It has a molecular weight of 24.9 kDa as determined by calculation.
Shipped with Ice Packs
Cat. No.
BT6637
Source

HEK293 cells.

Appearance
White powder that has been sterilized by filtration and lyophilized (freeze-dried).

Acrp30 (108-244) Human

Adiponectin (108-244 a.a.) Human Recombinant

Recombinant human Acrp30, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 158 amino acids (108-244). With a molecular weight of 18.1 kDa, this protein includes a 21 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT6165
Source
Escherichia Coli.

Acrp30 Human, HMW

Adiponectin glycosylated Human Recombinant, HMW Rich

Recombinant Human Adiponectin (HMW Rich), expressed in HEK cells, is a single-chain polypeptide comprising amino acids 19-244. This glycosylated protein has a molecular weight of 24.6 kDa (calculated) and consists of 226 amino acids. Under non-reducing conditions on SDS-PAGE, it migrates at approximately 884 kDa.

Shipped with Ice Packs
Cat. No.
BT6377
Source
HEK293.
Appearance
White powder, lyophilized (freeze-dried) and sterile-filtered.

Acrp30 Human, Sf9

Adiponectin Human Recombinant, Sf9

Recombinant Human Acrp30, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 146 amino acids (106-242a.a.). With a molecular mass of 16.9 kDa, it appears at approximately 13.5-18 kDa on SDS-PAGE. The protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT6474
Source
Sf9, Baculovirus cells.
Appearance
Clear, sterile-filtered solution.

gAcrp30 Mouse

Globular Adiponectin Mouse Recombinant

The recombinant globular domain of mouse Adiponectin/Acrp30, encompassing amino acid residues 111-247 with a molecular mass of 16 kDa, was overexpressed in E. coli and purified using standard chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT8067
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

ACRP30 Rat

Adiponectin Rat Recombinant

Recombinant Rat ACRP30, produced in a 293 cell line, is a polypeptide chain comprising 238 amino acids with a molecular weight of 25.7 kDa. This ACRP30 variant includes a C-terminal linker (3 amino acid residues) and a C-terminal Flag-tag (8 amino acid residues).
Shipped with Ice Packs
Cat. No.
BT7714
Source
HEK293 cell line.
Appearance
White, lyophilized powder.

gAcrp30 Human

Globular Adiponectin Human Recombinant

Recombinant human gAcrp30, produced in E. coli, is a non-glycosylated polypeptide chain comprising 145 amino acids. With a molecular weight of 16.7 kDa, this protein is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7860
Source
Escherichia Coli.

gAcrp30 Human, His

Globular Adiponectin Human Recombinant, His Tag

gAcrp30 Human has a total of 141 amino acids. N-terminal underlined amino acids are His-tag and the protease cleavage site (25AA-Underlined). The AA sequence of gAcrp30 Human is homologous to the 105-244 amino acid sequence of the Human full-length Adiponectin (Swiss-prot entry Q15848).
Shipped with Ice Packs
Cat. No.
BT7973
Source
Escherichia Coli.

Acrp30 Human, HEK

Adiponectin Human Recombinant, HEK

Recombinant human Acrp30, expressed in HEK293 cells, is engineered with a FLAG tag.
Shipped with Ice Packs
Cat. No.
BT6235
Source
HEK293 (Human Embryonic Kidney cell line).
Appearance
Lyophilized powder, white in color.
Definition and Classification

Adiponectin is a protein hormone and adipokine primarily produced by adipose tissue. It plays a crucial role in regulating glucose levels and fatty acid breakdown. Adiponectin is classified as an adipokine, a type of cytokine secreted by adipose tissue, and is involved in various metabolic processes .

Biological Properties

Key Biological Properties: Adiponectin is known for its insulin-sensitizing and anti-inflammatory effects. It circulates in high concentrations in human plasma and exists in three major forms: trimers, hexamers, and high-molecular-weight (HMW) complexes .

Expression Patterns and Tissue Distribution: Adiponectin is primarily produced by adipocytes in white adipose tissue but is also expressed in skeletal muscle cells, heart muscle cells, and endothelial cells . It is found in various tissues, including subcutaneous fat, visceral fat, and bone marrow fat .

Biological Functions

Primary Biological Functions: Adiponectin plays a significant role in enhancing insulin sensitivity, reducing inflammation, and protecting against atherosclerosis. It also promotes fatty acid oxidation and glucose uptake in skeletal muscle .

Role in Immune Responses and Pathogen Recognition: Adiponectin modulates immune responses by interacting with macrophages and monocytes, key components of the innate immune system. It has anti-inflammatory properties and can influence the production of inflammatory cytokines .

Modes of Action

Mechanisms with Other Molecules and Cells: Adiponectin exerts its effects by binding to specific receptors, AdipoR1 and AdipoR2, present in various tissues such as muscle and liver . It stimulates glucose uptake and utilization in skeletal muscles and enhances fatty acid oxidation in the liver .

Binding Partners and Downstream Signaling Cascades: Adiponectin activates several signaling pathways, including AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPARα), which play crucial roles in metabolic regulation .

Regulatory Mechanisms

Control of Expression and Activity: The expression and activity of adiponectin are regulated by various hormones, including insulin, insulin-like growth factor (IGF-1), growth hormone (GH), and leptin . These hormones influence adiponectin synthesis and release from adipose tissue .

Transcriptional Regulation and Post-Translational Modifications: Adiponectin gene expression is regulated at the transcriptional level by factors such as PPARγ. Post-translational modifications, including glycosylation, are essential for the proper folding and secretion of adiponectin .

Applications

Biomedical Research: Adiponectin is extensively studied for its role in metabolic diseases, including obesity, type 2 diabetes, and cardiovascular disease. It serves as a biomarker for diagnosing these conditions and monitoring the effectiveness of treatments .

Diagnostic Tools and Therapeutic Strategies: Adiponectin levels are measured in clinical settings to assess metabolic health. Therapeutic strategies targeting adiponectin pathways are being explored to develop treatments for metabolic disorders .

Role in the Life Cycle

Development to Aging and Disease: Adiponectin levels vary throughout the life cycle. They are typically higher in lean individuals and decrease with obesity and aging. Low adiponectin levels are associated with increased risk of metabolic diseases, including type 2 diabetes and cardiovascular disease .

Adiponectin plays a protective role in various stages of life, contributing to metabolic homeostasis and reducing inflammation. Its role in aging and disease highlights its potential as a therapeutic target for age-related metabolic disorders .

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