TSLP Human, His

Thymic Stromal Lymphopoietin Human Recombinant, His Tag

Recombinant human TSLP, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids Tyr29 to Gln159, including a 10-amino acid Histidine tag at the N-terminus. The calculated molecular mass is 16.2 kDa.
Shipped with Ice Packs
Cat. No.
BT23592
Source
Escherichia Coli.
Appearance
White, lyophilized powder.

TSLP Mouse

Thymic Stromal Lymphopoietin Mouse Recombinant

Recombinant Mouse TSLP, produced in a Baculovirus expression system, is a single, glycosylated polypeptide chain comprising 130 amino acids (residues 20-140). With a molecular weight of 15.0 kDa, the protein includes a 6-amino acid His-tag fused to its C-terminus. Purification is achieved through proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT23680
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

TSLPR Human

Thymic Stromal Lymphopoietin Receptor Human Recombinant

Recombinant human TSLPR, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 232 amino acids (residues 23-231). It has a molecular weight of 26.6 kDa. The TSLPR protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23774
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

TSLPR Human, Sf9

Thymic Stromal Lymphopoietin Receptor Human Recombinant, Sf9

Recombinant human TSLPR, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It contains 218 amino acids (residues 23-231), resulting in a molecular mass of 25.2 kDa. Note: On SDS-PAGE, the apparent molecular size may appear between 28-40 kDa due to glycosylation. This TSLPR protein is tagged with a 6-amino acid His-tag at the C-terminus and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT23882
Source
Sf9, Baculovirus cells.
Appearance
Clear, sterile-filtered solution.

TSLP Human

Thymic Stromal Lymphopoietin Human Recombinant

Recombinant human TSLP, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 132 amino acids. It has a molecular weight of 15 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.
Shipped with Ice Packs
Cat. No.
BT23506
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Definition and Classification

Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-2-like cytokine, alarmin, and growth factor involved in numerous physiological and pathological processes, primarily those of the immune system . It shares a common ancestor with IL-7 . TSLP is classified as a pleiotropic cytokine due to its ability to exert multiple effects on various cell types .

Biological Properties

Key Biological Properties: TSLP is a pleiotropic cytokine that plays a critical role in the development and progression of allergy and asthma . It is primarily produced by epithelial cells and functions as a potent immune system activator .

Expression Patterns: TSLP is primarily expressed by activated lung and intestinal epithelial cells, keratinocytes, and fibroblasts . Dendritic cells (DCs), mast cells, and other immune cells can also produce TSLP .

Tissue Distribution: TSLP is expressed in various tissues, including the lungs, skin, and intestines . It is also found in the pancreatic epithelial cells, gallbladder, cartilage tissue, liver, heart, urinary bladder, prostate, and coronary artery .

Biological Functions

Primary Biological Functions: TSLP is involved in the regulation of signaling receptor activity, interleukin-7-mediated signaling pathway, and positive regulation of cytokine-mediated signaling pathways . It also plays a role in the positive regulation of cell population proliferation, chemokine production, and interleukin production .

Role in Immune Responses: TSLP is a key player in the immune response to environmental insults, initiating a number of downstream inflammatory pathways . It activates dendritic cells to promote T helper (Th) 2 immune responses and directly promotes Th2 differentiation of naïve CD4+ T cells .

Pathogen Recognition: TSLP is involved in the defense response to Gram-negative bacteria and fungi .

Modes of Action

Mechanisms with Other Molecules and Cells: TSLP exerts its biological effects by binding to a high-affinity heteromeric complex composed of the thymic stromal lymphopoietin receptor (TSLPR) chain and IL-7Rα . This binding leads to the activation of Janus kinase (JAK)1 and 2, which in turn activates signal transducer and activator of transcription (STAT)5A and 5B, and to a lesser extent, STAT1 and 3 .

Binding Partners: TSLP binds to the TSLPR and IL-7Rα receptor complex .

Downstream Signaling Cascades: Upon binding to its receptor complex, TSLP activates JAK1 and 2, leading to the activation of STAT5A and 5B, and to a lesser extent, STAT1 and 3 .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of TSLP are regulated by various transcription factors, including NF-κB and AP-1 . Environmental factors such as Toll-like receptor ligands, viruses, microbes, allergens, and pollutants can trigger TSLP production . Proinflammatory cytokines, Th2-related cytokines, and IgE also induce or enhance TSLP production .

Transcriptional Regulation: TSLP mRNA expression is regulated transcriptionally by NF-κB and AP-1 .

Post-Translational Modifications: TSLP can be cleaved by several endogenous proteases in pathological conditions .

Applications

Biomedical Research: TSLP is a critical player in the development and progression of allergy and asthma, making it a valuable target for research .

Diagnostic Tools: TSLP levels can be measured to assess the severity of allergic diseases and asthma .

Therapeutic Strategies: Tezepelumab, a monoclonal antibody targeting TSLP, has been approved for the treatment of severe asthma . Several clinical trials are evaluating the safety and efficacy of tezepelumab in different inflammatory disorders .

Role in the Life Cycle

Development: TSLP is involved in the proliferation and development of immature B cells . It also promotes Th2 and Treg cells required for a successful pregnancy .

Aging and Disease: TSLP plays a role in chronic inflammatory and autoimmune disorders, such as chronic obstructive pulmonary disease, celiac disease, psoriasis, and rheumatoid arthritis . It is also implicated in certain cancers .

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