Product List

Apo D Human

Apolipoprotein-D Human Recombinant

Apolipoprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82kDa.
The protein a.a sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were made:
Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein’s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10782
Source
Escherichia Coli.
Appearance
Filtered White lyophilized (freeze-dried) powder.

APO D Human, GST

APO-D Human Recombinant, GST Tag

APO-D Human Recombinant full length protein expressed in E.coli, shows a 48 kDa band on SDS-PAGE.
The APO-D is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10879
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

APOA1

Apolipoprotein A-I Human

APOA1 Human isolated from Human HDL is a single, glycosylated, polypeptide chain having a molecular mass of 28.3kDa. APOA1 is purified using delipidation and gel permeation chromatographic technique.
Shipped with Ice Packs
Cat. No.
BT10957
Source
Human HDL.
Appearance
Sterile filtered colorless solution.

APOA1 Human

Apolipoprotein A-I Human Recombinant

Apolipoprotein A-I Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 243 amino acids and having a molecular mass of 28.1kDa.
The APOA1 is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT11048
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

APOA1 Human, His

Apolipoprotein A-I Human Recombinant, His Tag

APOA1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 264 amino acids (25-267 a.a.) and having a molecular mass of 30.3kDa. APOA1 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11134
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

APOA1 Mouse

Apolipoprotein A-I Mouse Recombinant

APOA1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (25-264a.a) and having a molecular mass of 30.3kDa.
APOA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11265
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

APOA5 Human

Apolipoprotein A-V Human Recombinant

APOA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 366 amino acids (24-366 a.a.) and having a molecular mass of 41.3kDa.
APOA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11359
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

APOA5 Human, HEK

Apolipoprotein A-V Human Recombinant, HEK

APOA5 Human Recombinant Flag-Tagged Fusion Protein is 40.1 kDa protein containing 354 amino acid residues of the APOA5 Human and 11 additional amino acid residues of flagTag.
Shipped with Ice Packs
Cat. No.
BT11431
Source
HEK293
Appearance

Filtered White lyophilized (freeze-dried) powder.

APOB Human

Apolipoprotein-B Human

Human APOB produced from Human plasma having a molecular mass of 550 kDa.                     

Shipped with Ice Packs
Cat. No.
BT11495
Source

Human Plasma.

Appearance

Sterile Filtered lyophilized (freeze-dried) powder.

APOC1 Human

Apolipoprotein C-I Human Recombinant

APOC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids (27-83 a.a.) and having a molecular mass of 9.0kDa. APOC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11563
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Apolipoproteins are proteins that bind lipids (oil-soluble substances such as fats, cholesterol, and fat-soluble vitamins) to form lipoproteins . They play a crucial role in the transport of lipids in blood, cerebrospinal fluid, and lymph . Apolipoproteins are classified into several classes designated by letters, such as A, B, C, D, and E, with specific class members typically identified by a number . For example, Apolipoprotein A-I (apoA-I) is the major structural protein component of high-density lipoproteins (HDL) .

Biological Properties

Key Biological Properties: Apolipoproteins have amphipathic properties, allowing them to stabilize lipoprotein structure and solubilize lipid components . They also serve as enzyme cofactors for specific enzymes involved in lipid metabolism .

Expression Patterns and Tissue Distribution: Apolipoproteins are primarily synthesized in the liver and intestines . For instance, ApoA-I is mainly produced in the liver and intestines, while ApoE is synthesized in the liver and brain .

Biological Functions

Primary Biological Functions: Apolipoproteins are essential for lipid transport and metabolism . They stabilize lipoprotein particles, serve as enzyme cofactors, and act as ligands for lipoprotein receptors .

Role in Immune Responses and Pathogen Recognition: Apolipoproteins, such as ApoA-I, have anti-inflammatory properties and can modulate immune cell functions . They also play a role in pathogen recognition and clearance .

Modes of Action

Mechanisms with Other Molecules and Cells: Apolipoproteins interact with lipoprotein receptors and lipid transport proteins, facilitating lipoprotein uptake and clearance . They also serve as enzyme cofactors for lipid metabolism .

Binding Partners and Downstream Signaling Cascades: Apolipoproteins bind to various receptors, such as the low-density lipoprotein (LDL) receptor, and initiate downstream signaling cascades that regulate lipid metabolism .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: Apolipoprotein synthesis is regulated by dietary composition, hormones (insulin, glucagon, thyroxin, estrogens, androgens), alcohol intake, and various drugs (statins, niacin, and fibric acids) .

Transcriptional Regulation and Post-Translational Modifications: Transcription of apolipoproteins is controlled by promoter and enhancer elements, as well as epigenetic mechanisms . Post-translational modifications, such as glycosylation, also play a role in their function .

Applications

Biomedical Research: Apolipoproteins are studied for their roles in lipid metabolism, cardiovascular diseases, and neurodegenerative disorders .

Diagnostic Tools: Apolipoproteins, such as ApoA-I and ApoB, are used as biomarkers for cardiovascular risk assessment .

Therapeutic Strategies: Apolipoprotein mimetics and gene therapy are being explored as potential treatments for cardiovascular and inflammatory diseases .

Role in the Life Cycle

Role Throughout the Life Cycle: Apolipoproteins play a critical role in lipid metabolism from development to aging . They are involved in various physiological processes, including brain development, immune responses, and maintenance of lipid homeostasis .

In Aging and Disease: Dysregulation of apolipoproteins is associated with age-related diseases, such as atherosclerosis, Alzheimer’s disease, and metabolic disorders .

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