Product List

Flt3 Ligand Human

Flt3 Ligand Human Recombinant

Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22709
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Flt3 Ligand Human, HEK

Flt3-Ligand Human Recombinant, HEK derived

Flt3-Ligand Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 24-30kDa due to glycosylation. The Flt3-Ligand is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT22792
Source
HEK293 (Human Embryonic Kidney cell line).
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Flt3 Ligand Human, Sf9

Flt3 Ligand Human Recombinant, Sf9

Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22894
Source
Baculovirus Sf9 cells.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Flt3 Ligand Mouse

Flt3 Ligand Mouse Recombinant

Flt3-Ligand Mouse Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 163 amino acids and having a molecular mass of 18.6kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT22968
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Flt3 Ligand Mouse, Sf9

Flt3 Ligand Mouse Recombinant, Sf9

Flt3 Ligand produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (27-189 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 169 amino acids and having a molecular mass of 19.3kDa.
Flt3 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23068
Source
Sf9, Baculovirus cells.
Appearance
Sterile filtered colorless solution.

Flt3 Ligand Porcine

Flt3 Ligand Porcine Recombinant

Flt3-Ligand Porcine Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.3kDa.  

Flt3-Ligand is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT23145
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Flt3-Ligand Rhesus Macaque

Flt3-Ligand Rhesus Macaque Recombinant

Flt3 Ligand Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 18.0kDa.
The Flt3 Ligand Rhesus Macaque is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23210
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Introduction

Definition and Classification

Flt3 Ligand (Fms-like tyrosine kinase 3 ligand) is a hematopoietic cytokine that plays a crucial role in the proliferation and differentiation of various blood cell progenitors. It is structurally homologous to stem cell factor (SCF) and colony-stimulating factor 1 (CSF-1) . Flt3 Ligand is classified as a member of the class III receptor tyrosine kinase family, which includes other members like FMS, platelet-derived growth factor receptor (PDGFR), and KIT .

Biological Properties

Key Biological Properties: Flt3 Ligand is a four-helical bundle cytokine that stimulates the proliferation and differentiation of hematopoietic progenitor cells . It is expressed as both a transmembrane molecule and a soluble factor, allowing for local control of hematopoietic stem cell (HSC) stimulation .

Expression Patterns: Flt3 Ligand is expressed in various tissues, including the bone marrow, spleen, thymus, and lymph nodes . It is also found in granulocytes, lymphocytes, and other immune cells .

Tissue Distribution: The expression of Flt3 Ligand is predominantly found in hematopoietic tissues such as the bone marrow and spleen, as well as in the thymus and lymph nodes .

Biological Functions

Primary Biological Functions: Flt3 Ligand functions as a cytokine and growth factor that increases the number of immune cells, including B cells and T cells, by activating hematopoietic progenitors . It plays a critical role in the development of the immune system and the maintenance of HSCs .

Role in Immune Responses: Flt3 Ligand is essential for the differentiation and proliferation of dendritic cells, which are crucial for antigen presentation and the initiation of immune responses .

Pathogen Recognition: By promoting the development of dendritic cells and other immune cells, Flt3 Ligand enhances the body’s ability to recognize and respond to pathogens .

Modes of Action

Mechanisms with Other Molecules and Cells: Flt3 Ligand binds to its receptor, Flt3 (CD135), which is expressed on hematopoietic progenitor cells . Upon binding, Flt3 undergoes homodimerization and activation, leading to increased cellular signaling via multiple pathways, including RAS/MAPK, JAK/STAT, and PI3K/AKT .

Binding Partners: Flt3 Ligand primarily binds to the Flt3 receptor, which is found on multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells .

Downstream Signaling Cascades: The activation of Flt3 leads to the phosphorylation of downstream molecules, promoting cellular proliferation, inhibition of apoptosis, and inhibition of differentiation .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of Flt3 Ligand are regulated by various mechanisms, including transcriptional regulation and post-translational modifications . Dysregulated sphingolipid metabolism has been shown to contribute to cancer progression and poor therapeutic response in FLT3-mutated acute myeloid leukemia (AML) .

Transcriptional Regulation: The transcription of the Flt3 Ligand gene is controlled by various transcription factors that respond to cellular signals and environmental cues .

Post-Translational Modifications: Flt3 Ligand undergoes post-translational modifications, such as glycosylation, which are essential for its proper function and localization .

Applications

Biomedical Research: Flt3 Ligand is widely used in research to study hematopoiesis and immune system development . It is also used to generate dendritic cells for immunotherapy studies .

Diagnostic Tools: The expression levels of Flt3 Ligand and its receptor are used as biomarkers for certain hematologic malignancies, including AML .

Therapeutic Strategies: Flt3 Ligand has potential therapeutic applications in stem cell transplantation and cancer immunotherapy . FLT3 inhibitors are being developed to target FLT3 mutations in AML .

Role in the Life Cycle

Development: Flt3 Ligand is crucial for the early stages of hematopoiesis, promoting the development of HSCs and immune cells .

Aging: The expression and function of Flt3 Ligand may decline with age, potentially affecting the maintenance of HSCs and immune function .

Disease: Mutations in the Flt3 receptor are associated with various hematologic malignancies, including AML . Targeting these mutations with FLT3 inhibitors is a promising therapeutic strategy .

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