EBI3 Human

Epstein Barr Virus Induced 3 Human Recombinant

Recombinant Human EBI3, produced in E. coli, is a single, non-glycosylated polypeptide chain. This chain comprises 209 amino acids (fragment 21-229) and has a molecular weight of 23.3 kDa. The purification of EBI3 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT20754
Source
Escherichia Coli.
Appearance
White, sterile-filtered powder that has been lyophilized (freeze-dried).

EBI3 Human, His

Epstein Barr Virus Induced 3 Human Recombinant, His Tag

Recombinant human EBI3 is produced in E. coli. It is a single, non-glycosylated polypeptide chain containing amino acids 21-229 of EBI3 and a 4.5kDa amino-terminal hexahistidine tag. This results in a protein fragment with a molecular weight of 34kDa. EBI3 is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20859
Source
Escherichia Coli.
Appearance
The product is a clear solution that has been sterilized by filtration.

EBI3 Macaque

Epstein Barr Virus Induced 3 Macaque Recombinant

Recombinant Macaque EBI3, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 210 amino acids, resulting in a molecular mass of 23.4 kDa. The purification of EBI3 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT21451
Source

Escherichia Coli.

Appearance
Sterile Filtered White lyophilized powder

EBI3 Mouse

Epstein Barr Virus Induced 3 Mouse Recombinant

Recombinant Mouse EBI3, produced in E. coli, is a single, non-glycosylated polypeptide chain. This protein comprises 207 amino acids, resulting in a molecular mass of 22.9 kDa. The purification of Murine EBI3 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT21556
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Definition and Classification

EBI3, or Epstein-Barr virus-induced gene 3, is a protein encoded by the EBI3 gene in humans. It is also known as interleukin-27 subunit beta (IL-27B) and interleukin-35 subunit beta (IL-35B). EBI3 is a member of the hematopoietin receptor family and is related to the p40 subunit of interleukin-12 (IL-12). It forms part of two distinct heterodimeric cytokines: interleukin-27 (IL-27) and interleukin-35 (IL-35) .

Biological Properties

EBI3 is a secreted glycoprotein that plays a crucial role in immune responses. It is expressed in various tissues, including the placenta, spleen, lymph nodes, and appendix. The expression of EBI3 is induced in B lymphocytes by Epstein-Barr virus infection and is also regulated by Toll-like receptor (TLR) signaling in dendritic cells .

Biological Functions

EBI3 is involved in regulating cell-mediated immune responses. It forms part of IL-27 and IL-35, which have distinct roles in the immune system. IL-27, composed of EBI3 and p28, can trigger signaling in T cells, B cells, and myeloid cells, promoting T-helper cell development and suppressing T-cell proliferation. IL-35, composed of EBI3 and p35, is involved in regulatory T-cell function and has inhibitory effects on immune responses .

Modes of Action

EBI3 interacts with other molecules and cells through its role in forming IL-27 and IL-35. IL-27 binds to the cytokine receptor WSX-1/TCCR, triggering downstream signaling cascades that involve the JAK/STAT pathway. IL-35 also signals through the JAK/STAT pathway, modulating the activity of regulatory T cells and suppressing inflammatory responses. Additionally, EBI3 can bind to cytokines such as IFN-γ and IL-10, acting as a cytokine sink and limiting their functions .

Regulatory Mechanisms

The expression and activity of EBI3 are regulated at multiple levels. Transcriptional regulation involves NF-κB and Ets binding sites, which are activated by TLR signaling. Post-translational modifications, such as glycosylation, also play a role in the stability and function of EBI3. Furthermore, EBI3 collaborates with the chaperone protein calnexin to promote the proper folding and expression of target proteins under inflammatory conditions .

Applications

EBI3 has several applications in biomedical research, diagnostic tools, and therapeutic strategies. It is used in studies investigating immune responses and inflammatory diseases. EBI3 is also a target for developing diagnostic assays for conditions such as autoimmune diseases and certain cancers. Therapeutically, modulating EBI3 activity has potential in treating inflammatory and immune-related disorders .

Role in the Life Cycle

EBI3 plays a role throughout the life cycle, from development to aging and disease. During development, EBI3 is involved in the regulation of immune responses and the proper folding of cytokines. In aging, EBI3 expression can be altered, contributing to changes in immune function. In disease, dysregulation of EBI3 is associated with conditions such as autoimmune diseases, cancers, and chronic inflammatory disorders .

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