BMP 7 Human
Description
BMP-7 regulates diverse processes through Smad-dependent and Smad-independent pathways:
Key Roles
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Osteogenesis: Induces mesenchymal stem cell differentiation into osteoblasts via Smad1/5/8 phosphorylation and Runx2 activation .
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Organ Development: Critical for kidney morphogenesis, eye formation, and neural tube patterning .
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Anti-Fibrotic Activity: Reverses epithelial-to-mesenchymal transition (EMT) in renal and cardiac tissues .
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Immunomodulation: Modulates Langerhans cell differentiation and T-cell polarization .
Signaling Pathways
Approved Uses
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Orthopedics: rhBMP-7 (OP-1) is FDA-approved for long bone non-unions and spinal fusion .
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Renal Disease: Reduces fibrosis in chronic kidney injury models .
Emerging Applications
Recent Advances
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Fibrosis Reversal: BMP-7 suppresses TGF-β1-driven fibrosis in lung, liver, and peritoneal tissues .
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Cancer Biology: Dual role observed: inhibits metastasis in prostate cancer but promotes aggressiveness in gastric malignancies .
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Immunotherapy: BMP-7-generated Langerhans cells exhibit superior T-cell stimulatory capacity compared to TGF-β1-induced cells .
Comparative Efficacy
| Therapeutic Agent | Advantage | Limitation |
|---|---|---|
| rhBMP-7 | Low immunogenicity; broad anti-fibrotic effects | Less potent osteoinduction vs. rhBMP-2 |
| rhBMP-2 | Faster bone regeneration | Higher risk of heterotopic ossification |
Detection and Quantification
The Quantikine™ ELISA kit detects BMP-7 in serum, plasma, and urine with a sensitivity of 2.44 pg/mL . Recovery rates exceed 90% across matrices, enabling reliable clinical monitoring .
Challenges and Future Directions
While BMP-7’s pleiotropic effects offer therapeutic promise, challenges include:
Product Specs
Product Science Overview
Bone Morphogenetic Protein-7 (BMP-7), also known as Osteogenic Protein-1 (OP-1), is a member of the Transforming Growth Factor-beta (TGF-β) superfamily. This group of proteins is known for their role in regulating cell growth, differentiation, and apoptosis. BMP-7 is particularly notable for its osteoinductive properties, meaning it can induce the formation of bone and cartilage. It plays a crucial role in the development of the kidney, eye, and limb, as well as in the repair of bone fractures .
BMP-7 was first identified by its ability to induce endochondral osteogenesis in vivo at extraskeletal sites. This discovery was made by Professor Marshall Urist and Professor Hari Reddi, who demonstrated that demineralized bone extract could induce ectopic bone formation in rats . BMP-7 functions by promoting the differentiation of mesenchymal cells into bone and cartilage cells. It is a secreted multifunctional growth factor that forms a disulfide-linked homodimer, which is essential for its biological activity .
Recombinant human BMP-7 (rhBMP-7) is produced using recombinant DNA technology. This involves cloning the BMP-7 gene into a suitable expression system, such as mammalian cells, to produce the protein in large quantities. The recombinant protein is then purified and used for various medical applications. rhBMP-7 has been approved by the Food and Drug Administration (FDA) for specific uses, including spinal fusions and the treatment of nonunion fractures .
rhBMP-7 is used in orthopedic applications such as spinal fusions, nonunions, and oral surgery. It has also shown potential in the treatment of chronic kidney disease (CKD). Studies have demonstrated that BMP-7 can reverse the loss of glomeruli due to sclerosis in murine models . Additionally, BMP-7 has been used in clinical trials for bone repair and has shown promising results in promoting bone formation and healing .
The production of rhBMP-7 involves several steps:
- Gene Cloning: The full-length sequence of BMP-7 is amplified and cloned into a suitable vector.
- Expression: The vector is introduced into mammalian cells, such as CHO or 293T cells, which then produce the BMP-7 protein.
- Purification: The protein is purified from the conditioned medium of the cells. This often involves multiple chromatography steps to ensure high purity.
- Characterization: The purified protein is characterized to confirm its identity, purity, and biological activity. This includes tests such as western blotting, glycosylation analysis, and bioactivity assays .
