CHI3L1 (22-383) Human

Chitinase 3-Like 1 (22-383 a.a) Human Recombinant
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Cat. No.
BT30872
Source
Sf9, Baculovirus cells.
Synonyms
Chitinase 3 Like 1, Chitinase 3-Like 1 (Cartilage Glycoprotein-39), Cartilage Glycoprotein 39, 39 KDa Synovial Protein, HCGP-39, CGP-39, YKL-40, GP-39 , Chitinase-3-Like Protein 1, Cartilage Glycoprotein-39, HC-Gp39, HCGP-3P, YYL-40, ASRT7, YKL40.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Biological Functions

Mechanisms and Pathways (from ):

Key Roles in Physiology and Pathology

ProcessMechanismClinical Relevance
InflammationRegulates Th1/Th2 balance via IFN-γ/STAT1 axis; inhibits NF-κB signalingAsthma, rheumatoid arthritis
Tissue RemodelingModulates MMP activity (e.g., suppresses MMP-1/3/13; enhances MMP-9)Liver fibrosis, osteoarthritis
Cancer ProgressionActivates MAPK/Akt pathways; promotes angiogenesis and metastasisGlioblastoma, colon cancer, breast cancer
Insulin SensitivityCounteracts TNFα-induced insulin resistance via PAR2Obesity-related metabolic disorders

Receptor Interactions

ReceptorSignaling Pathway ActivatedFunctional Outcome
IL-13Rα2MAPK/Erk1/2, PI3K/AktCell proliferation, angiogenesis
CD44Integrin αvβ3/β5-Syndecan-1ECM adhesion, tumor invasion
Galectin-3STAT3 activationM2 macrophage polarization

Clinical and Research Applications

Diagnostic and Therapeutic Relevance ( ):

Biomarker Potential

DiseaseCorrelation with CHI3L1 LevelsPrognostic Value
Metastatic CancerElevated serum levelsShorter survival, poor prognosis
AsthmaAirway CHI3L1 linked to rs12141494 SNPSeverity predictor
Rheumatoid ArthritisSynovial fluid accumulationJoint damage progression

Research Tools ( ):

  • Quantikine® ELISA Kit: Measures CHI3L1 in serum/plasma (sensitivity: 0.16 ng/mL) .

  • Recombinant CHI3L1 (22-383): Used in studies on:

    • Tumor angiogenesis (U87 glioma xenografts) .

    • Insulin resistance rescue in skeletal muscle cells .

    • ECM remodeling assays (collagen degradation) .

Production and Quality Control

Recombinant Expression ( ):

  • Host System: HEK293 cells ensure proper glycosylation and folding.

  • Yield: Typically 0.5–2 mg/L in culture supernatants.

  • Endotoxin Levels: <1 EU/μg (LAL assay).

Functional Validation ( ):

  • Bioactivity: Confirmed via:

    • Rescue of TNFα-induced insulin resistance in myotubes .

    • Stimulation of bronchial smooth muscle cell proliferation .

Future Directions

  • Targeted Therapies: Anti-CHI3L1 monoclonal antibodies (e.g., FRGxCO in glioblastoma) .

  • Gene Editing: CRISPR-based knockdown to study metastasis suppression .

  • Multi-Omics Integration: Correlate CHI3L1 SNPs (e.g., rs10399931) with drug responses .

Product Specs

Introduction
Chitinase 3-Like 1 (CHI3L1) is an enzyme that typically breaks down chitin, a complex sugar found in insect exoskeletons and fungal cell walls. However, human CHI3L1 lacks this chitinase activity. Instead, it plays a role in inflammation and tissue remodeling. Produced by various cells like macrophages, chondrocytes, neutrophils, and synovial cells, CHI3L1 belongs to the glycosyl hydrolase 18 family.
Description
This product consists of the CHI3L1 protein, specifically amino acids 22 to 383 of the human sequence. It's produced using Sf9 insect cells modified with a baculovirus system. The protein is a single chain with glycosylation, weighing approximately 41.4 kDa. When analyzed by SDS-PAGE under reducing conditions, it appears between 40-57 kDa due to the glycosylation. For purification, proprietary chromatographic techniques are used. An 8 amino acid His tag is present at the C-terminus to aid in this process.
Physical Appearance
A clear, colorless liquid without any particles, sterilized by filtration.
Formulation
The CHI3L1 protein is provided at a concentration of 0.25mg per milliliter. It's dissolved in a solution containing Phosphate Buffered Saline at a pH of 7.4, 10% glycerol, and 1mM DTT.
Stability
For optimal short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For longer periods, store the protein frozen at -20°C. To ensure stability during extended storage, consider adding a carrier protein such as HSA or BSA (0.1% concentration). Repeated freezing and thawing of the product should be avoided.
Purity
Determined by SDS-PAGE analysis, the purity of this CHI3L1 protein is greater than 90%.
Synonyms
Chitinase 3 Like 1, Chitinase 3-Like 1 (Cartilage Glycoprotein-39), Cartilage Glycoprotein 39, 39 KDa Synovial Protein, HCGP-39, CGP-39, YKL-40, GP-39 , Chitinase-3-Like Protein 1, Cartilage Glycoprotein-39, HC-Gp39, HCGP-3P, YYL-40, ASRT7, YKL40.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
YKLVCYYTSW SQYREGDGSC FPDALDRFLC THIIYSFANI SNDHIDTWEW NDVTLYGMLN TLKNRNPNLK TLLSVGGWNF GSQRFSKIAS NTQSRRTFIK SVPPFLRTHG FDGLDLAWLY PGRGDKQHFT TLIKEMKAEF IKEAQPGKKQ LLLSAALSAG KVTIDSSYDI AKISQHLDFI SIMTYDFHGA WRGTTGHHSP LFRGQEDASP DRFSNTDYAV GYMLRLGAPA SKLVMGIPTF GRSFTLASSE TGVGAPISGP GIPGRFTKEA GTLAYYEICD FLRGATVHRI LGQQVPYATK GNQWVGYDDQ ESVKSKVQYL KDRQLAGAMV WALDLDDFQG SFCGQDLRFP LTNAIKDALA ATLEHHHHHH.

Product Science Overview

Structure and Expression

The recombinant form of Chitinase 3-Like 1 (22-383 a.a) is a fragment of the full-length protein, spanning amino acids 22 to 383 . This fragment is often expressed in E. coli or other expression systems and is tagged with various markers such as His-SUMO or Myc for purification and detection purposes . The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications .

Biological Functions

CHI3L1 is secreted by a variety of cell types, including:

  • Articular chondrocytes
  • Synovial cells
  • Activated monocyte-derived macrophages
  • Neutrophils
  • Endothelial cells
  • Vascular smooth muscle cells
  • Certain cancer cells

This protein plays a crucial role in:

  • Inflammatory responses: It is involved in the regulation of inflammation and has been implicated in diseases such as asthma, rheumatoid arthritis, and inflammatory bowel disease .
  • Tissue remodeling: CHI3L1 is involved in the remodeling of extracellular matrix components, which is essential for wound healing and tissue repair .
  • Cancer progression: Elevated levels of CHI3L1 have been associated with various cancers, including breast, lung, and colorectal cancers .
Applications in Research

Recombinant CHI3L1 is widely used in research to study its role in various biological processes and diseases. It is utilized in:

  • Enzyme-linked immunosorbent assays (ELISA)
  • Western blotting (WB)
  • Immunoprecipitation (IP)

These applications help researchers understand the protein’s function, its interactions with other molecules, and its potential as a therapeutic target.

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CHI3L1 Human
Chitinase 3-Like 1 Human Recombinant
Cat. No.
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Source
CHO (chinese hamster ovary cells).
THE BIOTEK
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