CHI3L1 (22-383) Human

Chitinase 3-Like 1 (22-383 a.a) Human Recombinant

This product consists of the CHI3L1 protein, specifically amino acids 22 to 383 of the human sequence. It's produced using Sf9 insect cells modified with a baculovirus system. The protein is a single chain with glycosylation, weighing approximately 41.4 kDa. When analyzed by SDS-PAGE under reducing conditions, it appears between 40-57 kDa due to the glycosylation. For purification, proprietary chromatographic techniques are used. An 8 amino acid His tag is present at the C-terminus to aid in this process.
Shipped with Ice Packs
Cat. No.
BT30872
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless liquid without any particles, sterilized by filtration.

CHI3L1 Human

Chitinase 3-Like 1 Human Recombinant

This recombinant CHI3L1 protein is produced in Chinese Hamster Ovary (CHO) cells. It is a single polypeptide chain with 408 amino acids (amino acids 1-383 of the human protein), a molecular weight of 45.5 kDa, and glycosylation modifications. For purification purposes, a 25 amino acid myc-His-tag is attached to the C-terminus.
Shipped with Ice Packs
Cat. No.
BT30883
Source
CHO (chinese hamster ovary cells).
Appearance
Clear, colorless, and sterile-filtered solution.

CHI3L2 Human

Chitinase 3-Like 2 Recombinant

Recombinant Human CHI3L2, produced in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses amino acids Tyr27 to Leu390 (374 amino acids total), including a 10 amino acid Histidine tag located at the N-terminus. The calculated molecular mass is 42.1 kDa.
Shipped with Ice Packs
Cat. No.
BT30901
Source
Escherichia Coli.
Appearance
White powder, lyophilized (freeze-dried), and filtered.

CHI3L2 Human, Sf9

Chitinase 3-Like 2 Human Recombinant, Sf9

Produced in Sf9 Baculovirus cells, CHI3L2 is a single, glycosylated polypeptide chain comprising 372 amino acids (27-390a.a.) with a molecular mass of 41.9kDa. It's important to note that on SDS-PAGE, the molecular size will appear within the range of 40-57kDa. The CHI3L2 protein is expressed with an 8 amino acid His tag located at the C-Terminus and undergoes purification using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT30922
Source
Sf9, Baculovirus cells.
Appearance
The product is a sterile and colorless solution that has been filtered for purity.

Chitinase Protein

Chitinase Clostridium Paraputrificum Recombinant

This product is a recombinant Chitinase protein from Clostridium Paraputrificum, produced in E. coli. It is a single, non-glycosylated polypeptide chain with a His tag at the N-terminus. The protein has a molecular weight of 64.2kDa and is purified using chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30937
Source
Escherichia Coli.
Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Definition and Classification

Chitinases are hydrolytic enzymes that catalyze the breakdown of chitin, a long-chain polymer of N-acetylglucosamine, which is a component of the cell walls of fungi, the exoskeletons of arthropods, and the scales of fish . Chitinases are classified into two main types: endochitinases and exochitinases. Endochitinases randomly cleave chitin at internal sites, producing low molecular weight chitooligosaccharides, while exochitinases sequentially cleave off monomers or dimers from the ends of the chitin chains .

Biological Properties

Chitinases exhibit a variety of biological properties, including antimicrobial, antifungal, and insecticidal activities . They are expressed in a wide range of organisms, including bacteria, fungi, plants, and animals . In plants, chitinases are often induced in response to pathogen attack and are part of the plant’s defense mechanism . In animals, chitinases are found in tissues such as the stomach and lungs, where they play roles in digestion and immune responses .

Biological Functions

The primary biological functions of chitinases include the degradation of chitin for nutrient acquisition and defense against chitin-containing pathogens . In plants, chitinases contribute to the defense against fungal pathogens by degrading the chitin in fungal cell walls . In animals, chitinases are involved in immune responses, recognizing and breaking down chitin from pathogens . They also play roles in processes such as molting in arthropods and morphogenesis in fungi .

Modes of Action

Chitinases act by hydrolyzing the β-1,4-glycosidic bonds in chitin, resulting in the production of chitooligosaccharides . They interact with other molecules and cells through binding partners and downstream signaling cascades . For example, in plants, chitinase activity can trigger signaling pathways that lead to the production of other defense-related proteins . In animals, chitinases can bind to chitin-containing pathogens, facilitating their recognition and destruction by the immune system .

Regulatory Mechanisms

The expression and activity of chitinases are regulated at multiple levels, including transcriptional regulation and post-translational modifications . In plants, chitinase genes are often upregulated in response to pathogen attack through signaling pathways involving salicylic acid and jasmonic acid . Post-translational modifications, such as glycosylation, can also affect the stability and activity of chitinases .

Applications

Chitinases have a wide range of applications in biomedical research, agriculture, and industry . In agriculture, chitinases are used as biocontrol agents to protect crops from fungal pathogens and insect pests . In biomedical research, chitinases are studied for their potential roles in diagnosing and treating diseases such as asthma and fungal infections . They are also used in the production of chitooligosaccharides, which have various health benefits, including antimicrobial and anti-inflammatory properties .

Role in the Life Cycle

Throughout the life cycle of organisms, chitinases play crucial roles in development, aging, and disease . In fungi, chitinases are involved in cell wall remodeling during growth and morphogenesis . In arthropods, they are essential for molting and metamorphosis . In humans, chitinases are implicated in various diseases, including asthma and chronic obstructive pulmonary disease (COPD), where they are thought to contribute to inflammation and tissue remodeling .

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