HRASLS3 Human

HRAS-Like Suppressor 3 Human Recombinant (PLA2G16)

Recombinant human HRASLS3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 133 amino acids (1-133) with a molecular weight of 14.9 kDa. The purification of HRASLS3 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT21517
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

PLA2G10 Human

Secreted Phospholipase A2-X Human Recombinant

Recombinant Human Secreted Phospholipase A2-X is produced with an N-terminal HisTag fusion. This PLA2G10 His-Tagged Fusion Protein has a molecular weight of 15.5 kDa. It consists of 123 amino acid residues of the human secreted phospholipase A2-X and 16 additional amino acid residues from the HisTag (underlined).
Shipped with Ice Packs
Cat. No.
BT21614
Source
Escherichia Coli.
Appearance
Sterile Filtered lyophilized powder.

PLA2G12 Human

Secreted Phospholipase A2-XII Human Recombinant

Recombinant human secreted Phospholipase A2-XII was produced with an N-terminal His-Tag. The PLA2G12 His-Tagged Fusion Protein has a molecular weight of 20.6 kDa and consists of 167 amino acid residues from the human secreted phospholipase A2-XII protein and an additional 16 amino acid residues comprising the His-Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT21695
Source
Escherichia Coli.
Appearance
White lyophilized powder, filtered for purity.

PLA2G1B Human

Secreted Phospholipase A2-IB Human Recombinant

Recombinant Human Secreted Phospholipase A2-IB is produced with an N-terminal HisTag. The PLA2G1B His-Tagged Fusion Protein has a molecular weight of 16 kDa and consists of 126 amino acids from human secreted phospholipase A2-IB, along with an additional 16 amino acid HisTag sequence (underlined).
Shipped with Ice Packs
Cat. No.
BT21783
Source
Escherichia Coli.
Appearance
The product is provided as a lyophilized powder.

PLA2G2A Human

Secreted Phospholipase A2-IIA Human Recombinant

Recombinant Human Secreted Phospholipase A2-IIA is expressed with an N-terminal His-tag. This protein has a molecular weight of 15.8 kDa, comprising 124 amino acids of the human secreted phospholipase A2-IIA and a 16 amino acid His-tag.
Shipped with Ice Packs
Cat. No.
BT21848
Source
Escherichia Coli.
Appearance
Sterile filtered lyophilized (freeze-dried) powder.

PLA2G2D Human

Secreted Phospholipase A2-IID Human Recombinant

Recombinant human secreted phospholipase A2-IID was expressed with an N-terminal His-tag. The resulting PLA2G2D His-tagged fusion protein has a molecular weight of 16.4 kDa and consists of 125 amino acids from the human secreted phospholipase A2-IID protein and 16 additional amino acids comprising the His-tag.
Shipped with Ice Packs
Cat. No.
BT21947
Source
Escherichia Coli.
Appearance
Lyophilized powder, sterile-filtered.

PLA2G2E Human

Secreted Phospholipase A2-IIE Human Recombinant

This product is a recombinant human secreted phospholipase A2-IIE protein with an N-terminal His-Tag. With a molecular weight of 15.8 kDa, this fusion protein consists of 123 amino acid residues from the human secreted phospholipase A2-IIE and an additional 16 amino acid residues for the His-Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT22012
Source
Escherichia Coli.
Appearance
This product is provided as a sterile, lyophilized (freeze-dried) powder.

PLA2G5 Human

Secreted Phospholipase A2-V Human Recombinant

Recombinant Human Secreted Phospholipase A2-V was produced with an N-terminal His-Tag. The PLA2G5 His-Tagged Fusion protein has a molecular weight of 15.5 kDa. It consists of 118 amino acid residues from the human secreted phospholipase A2-V and an additional 16 amino acid residues for the His-Tag (underlined).
Shipped with Ice Packs
Cat. No.
BT22124
Source
Escherichia Coli.
Appearance
The product appears as a lyophilized (freeze-dried) powder.

PLA2G7 Human

Secreted Phospholipase A2-VII Human Recombinant

Recombinant Human PLA2G7, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises a 420 amino acid fragment (22-441) and has a molecular weight of 52.29 kDa, including a 4.5 kDa amino-terminal hexahistidine tag.
The purification of PLA2G7 is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22193
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.

PLA2G7 Human, HEK

Secreted Phospholipase A2-VII Human Recombinant, HEK

Recombinant Human PLA2G7, expressed in HEK293 cells, is a polypeptide chain encompassing amino acids 22-441. An 8 amino acid His-tag is fused to the C-terminus, resulting in a total of 428 amino acids. The purification of PLA2G7 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT22266
Source
HEK293 cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Secreted Phospholipase A2 (sPLA2) is a member of the phospholipase A2 superfamily, which comprises enzymes that hydrolyze the sn-2 acyl bond of glycerophospholipids to release fatty acids and lysophospholipids . sPLA2 enzymes are characterized by their low molecular weight, requirement for calcium ions (Ca²⁺) for activity, and a conserved His/Asp catalytic dyad . They are classified into several groups based on their structure and function, including groups IB, IIA, IIC, IID, IIE, IIF, III, V, X, XIIA, and XIIB .

Biological Properties

sPLA2 enzymes exhibit distinct biological properties, including specific expression patterns and tissue distribution. They are found in various mammalian tissues, such as the pancreas, kidney, and central nervous system . sPLA2 enzymes are also present in snake and insect venoms . Their expression can be induced during inflammation, and they play roles in metabolic regulation, including lipid metabolism and energy production .

Biological Functions

The primary biological functions of sPLA2 include the hydrolysis of phospholipids to produce fatty acids and lysophospholipids, which serve as precursors for bioactive lipid mediators such as prostaglandins and leukotrienes . sPLA2 enzymes are involved in immune responses, pathogen recognition, and host defense mechanisms . They contribute to inflammation, atherosclerosis, and other pathophysiological processes .

Modes of Action

sPLA2 enzymes interact with other molecules and cells through various mechanisms. They bind to specific receptors on cell surfaces, leading to the activation of downstream signaling cascades . The hydrolysis of phospholipids by sPLA2 results in the release of arachidonic acid, which is further metabolized into eicosanoids, potent mediators of inflammation . sPLA2 enzymes also modulate membrane composition and participate in lipid signaling .

Regulatory Mechanisms

The expression and activity of sPLA2 are tightly regulated by multiple mechanisms. Transcriptional regulation involves the activation of specific genes in response to inflammatory stimuli . Post-translational modifications, such as phosphorylation, can modulate the enzyme’s activity and localization . Calcium ions are essential for the catalytic activity of sPLA2, and their concentration can influence enzyme function .

Applications

sPLA2 enzymes have various applications in biomedical research, diagnostic tools, and therapeutic strategies. They are used as biomarkers for inflammatory diseases and cancer . Inhibitors of sPLA2 are being developed as potential therapeutic agents for conditions such as acute lung injury and atherosclerosis . sPLA2 enzymes are also studied for their roles in lipid metabolism and energy homeostasis .

Role in the Life Cycle

sPLA2 enzymes play crucial roles throughout the life cycle, from development to aging and disease. During development, they are involved in processes such as sperm maturation and capacitation . In adulthood, sPLA2 enzymes contribute to immune responses and metabolic regulation . In aging and disease, their dysregulation is associated with inflammatory conditions, metabolic disorders, and cancer .

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