KLK11 Human

Kallikrein-11 Human Recombinant

Recombinant human KLK11, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 226 amino acids (residues 54-278). It has a molecular weight of 24.8 kDa.
Shipped with Ice Packs
Cat. No.
BT3436
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

KLK11 Human, Sf9

Kallikrein-11, 4 Human Recombinant, Sf9

Produced in Sf9 insect cells, KLK11 is a single, glycosylated polypeptide chain comprising 241 amino acids (19-250a.a.). It has a molecular mass of 26.7 kDa; however, its apparent size on SDS-PAGE is estimated to be between 28-40 kDa. This KLK11 protein is expressed with a 9-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT3484
Source

Sf9, Insect cells.

Appearance
Sterile filtered solution, colorless.

KLK8 Mouse

Kallikrein-8 Mouse Recombinant

KLK8 Mouse Recombinant is a purified protein produced in Sf9 insect cells. It is a single, glycosylated polypeptide chain consisting of 240 amino acids (29-260) with a molecular weight of 26.5kDa. However, on SDS-PAGE, it appears between 28-40kDa due to glycosylation. This recombinant protein has an 8 amino acid His-Tag fused at its C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4717
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless liquid, free from particulate matter.

KLK1 Human, HEK

Kallikrein-1 Human Recombinant, HEK

KLK1 Human Recombinant, produced in HEK293 cells, is a single, glycosylated polypeptide chain consisting of 244 amino acids (Pro19-Ser262). It is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT2952
Source

HEK293 cells 

Appearance
Sterile Filtered clear solution.

KLK1 Human, His

Kallikrein-1 Human Recombinant, His Tag

Recombinant human Kallikrein-1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 259 amino acids (residues 25-262) and has a molecular weight of 28.7kDa. The KLK1 protein includes a 21 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT3004
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

KLK10 Human

Kallikrein-10 Human Recombinant

Recombinant human KLK10, expressed in E.coli, is available as a single, non-glycosylated polypeptide chain. This protein encompasses 266 amino acids (residues 34-276) with a molecular weight of 29.1kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus. The purification process utilizes proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3125
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless solution that has been filtered.

KLK10 Human, Sf9

Kallikrein-10 Human Recombinant, Sf9

Recombinant KLK10, expressed in Sf9 insect cells using a baculovirus system, is a single polypeptide chain with glycosylation. It encompasses amino acids 34 to 276, totaling 252 amino acids, and possesses a molecular weight of 27.8 kDa. This protein is engineered with a 6-amino acid His-tag at its C-terminus to facilitate purification, which is achieved through standard chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT2862
Source

Sf9, Baculovirus cells.

Appearance
The product is a sterile, colorless solution that has been filtered for sterility.

KLK2 Human, sf9

Kallikrein-2 Human Recombinant, sf9

Recombinant human KLK2, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. It encompasses 246 amino acids (residues 25-261) with a molecular weight of 27.2 kDa. On SDS-PAGE under reducing conditions, it migrates between 28-40 kDa. The protein includes a C-terminal 6-histidine tag for purification via proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3910
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless, and sterile-filtered solution.

KLK3 Human, HEK

Kallikrein-3 Human Recombinant, HEK

Recombinant human KLK3, produced in HEK cells, is a single-chain polypeptide. It undergoes glycosylation and consists of 250 amino acids (18-261 a.a), resulting in a molecular weight of 27.6 kDa.
For purification purposes, a 6-amino acid His-tag is fused to the C-terminus of KLK3, and proprietary chromatographic techniques are employed.

Shipped with Ice Packs
Cat. No.
BT4002
Source

HEK293 Cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

KLK3 Human, Native

Kallikrein-3 Human

Native human Kallikrein-3, isolated from human seminal fluid, with an approximate molecular weight of 30 kDa.

Shipped with Ice Packs
Cat. No.
BT4112
Source

Human seminal fluid.

Appearance

A clear, sterile-filtered solution.

Definition and Classification

Kallikreins are a subgroup of serine proteases, enzymes capable of cleaving peptide bonds in proteins. In humans, they are classified into two main types: plasma kallikrein and tissue kallikreins. Plasma kallikrein is encoded by the KLKB1 gene and has no known paralogue, while tissue kallikrein-related peptidases (KLKs) encode a family of fifteen closely related serine proteases . These genes are localized to chromosome 19q13, forming the largest contiguous cluster of proteases within the human genome .

Biological Properties

Key Biological Properties: Kallikreins are involved in various physiological functions, including blood pressure regulation, semen liquefaction, and skin desquamation .

Expression Patterns and Tissue Distribution: Kallikreins are expressed in many tissues, including the prostate, breast, ovary, and testis . They are also found in biological fluids such as seminal plasma, breast milk, and cerebrospinal fluid . Some kallikreins have restricted expression patterns, while others are widely distributed across different tissues .

Biological Functions

Primary Biological Functions: Kallikreins play a crucial role in regulating blood pressure, semen liquefaction, and skin desquamation . They are also involved in the activation of inflammation and the regulation of electrolyte balance .

Role in Immune Responses and Pathogen Recognition: Kallikreins are implicated in immune responses by activating kinins, which are peptides responsible for inflammation and vasodilation . They also play a role in pathogen recognition by modulating the activity of immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: Kallikreins interact with various molecules and cells through proteolytic cascades. For example, plasma kallikrein liberates kinins from kininogens, which regulate blood pressure and inflammation .

Binding Partners and Downstream Signaling Cascades: Kallikreins bind to kininogens to release kinins, which then activate B1 and B2 receptors, leading to downstream signaling cascades that regulate blood pressure, inflammation, and other physiological processes .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: Kallikrein expression is regulated by steroid hormones such as androgens, estrogens, and glucocorticoids . Their activity is controlled through zymogen activation, endogenous inhibitors like serpins, and internal (auto)cleavage leading to inactivation .

Transcriptional Regulation and Post-Translational Modifications: Kallikreins undergo transcriptional regulation by steroid hormones and post-translational modifications such as glycosylation, which affect their stability and activity .

Applications

Biomedical Research: Kallikreins are studied for their role in various physiological and pathological processes, including cancer, cardiovascular diseases, and neurodegenerative disorders .

Diagnostic Tools: Prostate-specific antigen (PSA; KLK3) and human glandular kallikrein (hK2) are used as tumor markers for prostate cancer . Other kallikreins are emerging as biomarkers for ovarian and breast cancers .

Therapeutic Strategies: Kallikrein inhibitors are being developed for the treatment of hereditary angioedema and other inflammatory conditions . Recombinant tissue kallikrein is also being explored for therapeutic use in ischemic stroke and renal disease .

Role in the Life Cycle

Role Throughout the Life Cycle: Kallikreins play a role in various stages of life, from development to aging and disease. They are involved in skin desquamation, seminal clot liquefaction, and neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases . Dysregulated kallikrein expression is associated with cancer progression and other age-related diseases .

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