Product List

CSNK2A1 Human, His

Casein Kinase 2 alpha 1 Human Recombinant, His

CK2A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain encoding the sequence of 411 amino acids and having a molecular mass of 47.3 kDa.
Casein Kinase 2 alpha subunit is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30125
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CSNK2A2 Human

Casein Kinase 2, Alpha 2 Prime Human Recombinant

CSNK2A2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 374 amino acids (1-350 a.a) and having a molecular mass of 43.7kDa.
CSNK2A2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30168
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

CSNK2B Human

Casein Kinase 2 beta Human Recombinant

CSNK2B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 215 amino acids (1-215 a.a) and having a molecular mass of 24.9 kDa.
CSNK2B is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT30221
Source

Escherichia Coli.

Appearance
Sterile Filtered colorless solution.

CSNK2B Protein

Casein Kinase 2b Human Recombinant

CSNK2B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 215 amino acids and having a total molecular mass of 24.9kDa.
CK2 beta is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30256
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

CK2a Zea Mays

Casein Kinase 2 alpha Zea Mays Recombinant

Casein Kinase 2 alpha Zea Mays Recombinant is a non-glycosylated polypeptide having a molecular mass of 39.2 kDa.
Casein Kinase 2 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29795
Source
Escherichia Coli.
Appearance

CK2h Human

Casein Kinase 2 Holoenzyme Human Recombinant

Human recombinant casein kinase 2 holo enzyme containing alpha and beta subunits which were separately expressed in E. coli as non-fusion proteins and purified using several chromatographic steps. The holo enzyme has been reconstituted in the course of the purification and is highly active suitable for labelling CK2 substrates. CK2 holoenzyme is a non-glycosilated polypeptide having a molecular mass of 140 kDa.
Shipped with Ice Packs
Cat. No.
BT29859
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CSNK1A1 Human

Casein Kinase 1 alpha 1 Human Recombinant

CSNK1A1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-337) and having a molecular mass of 41kDa. CSNK1A1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29913
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

CSNK1D Human

Casein Kinase 1 Delta Human Recombinant

CSNK1D Human Recombinant produced in E. coli is a single polypeptide chain containing 429 amino acids (1-409) and having a molecular mass of 48.9kDa.
CSNK1D is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29994
Source
Escherichia Coli.
Appearance
CSNK1D is supplied as a sterile filtered clear solution.

CSNK2A1 Human

Casein Kinase 2 alpha 1 Human Recombinant

Casein Kinase 2 alpha 1 Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 45.1 kDa.
Casein Kinase 2 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30061
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Casein kinases are a family of serine/threonine-specific protein kinases that play crucial roles in various cellular processes. They are classified into two main groups: Casein Kinase 1 (CK1) and Casein Kinase 2 (CK2). CK1 is further divided into several isoforms, including CK1α, CK1δ, CK1ε, and others, each with distinct functions and regulatory mechanisms .

Biological Properties

Key Biological Properties: Casein kinases are involved in regulating numerous cellular processes, including cell cycle progression, apoptosis, DNA repair, and circadian rhythms .

Expression Patterns: CK1 isoforms are ubiquitously expressed in eukaryotic cells, with varying levels depending on the tissue type and developmental stage .

Tissue Distribution: CK1 isoforms are found in various cellular compartments, including the cytoplasm, nucleus, and membranes. They are particularly abundant in tissues with high cellular turnover, such as the gastrointestinal tract, skin, and immune system .

Biological Functions

Primary Biological Functions: Casein kinases phosphorylate a wide range of substrates, influencing processes such as signal transduction, gene expression, and protein stability .

Role in Immune Responses and Pathogen Recognition: CK1α, for example, plays a role in the immune response by regulating the activation of nuclear factor κB (NF-κB), a key transcription factor involved in inflammation and immune responses .

Modes of Action

Mechanisms with Other Molecules and Cells: Casein kinases interact with various proteins and cellular structures. For instance, CK1α phosphorylates β-catenin, a crucial component of the Wnt signaling pathway, thereby regulating cell proliferation and differentiation .

Binding Partners and Downstream Signaling Cascades: CK1 isoforms have numerous binding partners, including transcription factors, receptors, and other kinases. These interactions often lead to the activation or inhibition of downstream signaling pathways, such as the Wnt/β-catenin, Hedgehog, and NF-κB pathways .

Regulatory Mechanisms

Expression and Activity Control: The expression and activity of casein kinases are tightly regulated at multiple levels, including transcriptional regulation, post-translational modifications, and interactions with regulatory proteins .

Transcriptional Regulation: Specific transcription factors and regulatory elements control the expression of CK1 and CK2 genes, ensuring their appropriate levels in different tissues and developmental stages .

Post-Translational Modifications: Casein kinases themselves undergo various post-translational modifications, such as phosphorylation, ubiquitination, and sumoylation, which modulate their activity, stability, and interactions with other proteins .

Applications

Biomedical Research: Casein kinases are valuable tools in biomedical research for studying cellular signaling pathways and disease mechanisms .

Diagnostic Tools: Altered expression or activity of casein kinases is associated with various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. Thus, they serve as potential biomarkers for disease diagnosis and prognosis .

Therapeutic Strategies: Targeting casein kinases with specific inhibitors or modulators holds promise for developing novel therapeutic strategies for treating cancer, autoimmune diseases, and other conditions .

Role in the Life Cycle

Development to Aging and Disease: Casein kinases play essential roles throughout the life cycle, from embryonic development to aging. They regulate key processes such as cell division, differentiation, and apoptosis, which are critical for normal development and tissue homeostasis . Dysregulation of casein kinase activity is implicated in various age-related diseases, including cancer and neurodegenerative disorders .

© Copyright 2024 Thebiotek. All Rights Reserved.