IDI1 Human

Isopentenyl-Diphosphate Delta Isomerase 1 Human Recombinant

This product consists of the recombinant human IDI1 protein, expressed in E. coli and purified to a high degree. It is a single, non-glycosylated polypeptide chain comprising 248 amino acids (residues 1-228) with a molecular weight of 28.6 kDa. The protein includes a 20 amino acid His-tag fused at the N-terminus to facilitate purification and detection.
Shipped with Ice Packs
Cat. No.
BT567
Source
E.coli.
Appearance
Clear, colorless solution, sterile-filtered.

IDI2 Human

Isopentenyl-Diphosphate Delta Isomerase 2 Human Recombinant

Produced in E.Coli, our IDI2 is a single, non-glycosylated polypeptide chain. It comprises 247 amino acids (specifically, amino acids 1 to 227) and has a molecular weight of 28.9kDa. For purification and ease of use, a 20 amino acid His-tag is fused to the N-terminus of IDI2. The protein is then purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT653
Source
Escherichia Coli.
Appearance
The product is a clear, colorless solution that has been sterilized through filtration.

MPI Human

Mannose Phosphate Isomerase Human Recombinant

Recombinant human MPI, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 382 amino acids (residues 1-362). It has a molecular weight of 41.9 kDa. The MPI protein is engineered with a 20 amino acid His-Tag at the N-terminus to facilitate purification by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT728
Source
E.coli.
Appearance
A clear, sterile-filtered solution.

RPIA Human

Ribose 5-Phosphate Isomerase A Human Recombinant

Recombinant human RPIA, expressed in E. coli, is available as a purified, non-glycosylated polypeptide chain. This protein consists of 331 amino acids, with amino acids 1-311 representing the RPIA sequence, and has a molecular weight of 35.4 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus.
Shipped with Ice Packs
Cat. No.
BT1905
Source
Escherichia Coli.
Appearance
A clear solution, sterilized by filtration.

SlyD E.Coli

FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase E.Coli Recombinant

Recombinant SlyD, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 196 amino acids. It has a molecular weight of 21 kDa.
Shipped with Ice Packs
Cat. No.
BT2075
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

SURA E.Coli

Chaperone SURA E.Coli Recombinant

Recombinantly produced in E. coli, SURA is a non-glycosylated polypeptide chain consisting of 429 amino acids (specifically, amino acids 21-428). With a molecular weight of 47.3 kDa, the SURA protein is engineered with a 20 amino acid His-Tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2133
Source
Escherichia Coli.
Appearance
A clear solution, free from particulate matter and sterilized by filtration.

TOP1 70kDa Human

DNA Topoisomerase-I 70kDa Recombinant Human

Recombinant TOP1 70kDa protein is a fragment of the TOP1 enzyme. It has a molecular weight of 72 kDa when measured at a pH of 9.4. A hexa-histidine tag is fused to this protein to facilitate purification.

Shipped with Ice Packs
Cat. No.
BT2189
Source
Sf9 insect cells.

PDIA4 Human

Protein Disulfide Isomerase A4 Human Recombinant

Recombinant human PDIA4, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 646 amino acids (specifically, amino acids 21 to 645) and has a molecular weight of 72.9 kDa. This PDIA4 variant is fused to a 21-amino acid His-Tag at its N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1234
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

PDIA4 Human, Active

Protein Disulfide Isomerase A4 Human Recombinant, Active

Recombinant human PDIA4, expressed in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 646 amino acids, specifically residues 21 to 645, with a molecular weight of 72.9 kDa. The N-terminus of PDIA4 is fused to a 21 amino acid His-Tag. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1323
Source
Escherichia Coli.
Appearance
The solution is colorless and has been sterilized through filtration.

PDIA6 Human

Protein Disulfide Isomerase A6 Human Recombinant

Recombinant human PDIA6, expressed in E. coli, is a non-glycosylated polypeptide chain with a His tag (21 amino acids) at its N-terminus. This single-chain protein consists of 442 amino acids (20-440 a.a.), resulting in a molecular weight of 48.5 kDa. Purification of PDIA6 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1417
Source
Escherichia Coli.
Appearance
A sterile, colorless solution.
Definition and Classification

Isomerases are a class of enzymes that catalyze the conversion of molecules from one isomer to another. This process involves intramolecular rearrangements where bonds are broken and formed, resulting in a product with the same molecular formula but different structural or spatial arrangements . Isomerases are classified into several subcategories based on the type of isomerization they catalyze:

  • Racemases and Epimerases: Catalyze the conversion of one stereoisomer to another.
  • Cis-trans Isomerases: Catalyze the conversion between cis and trans isomers.
  • Intramolecular Lyases: Catalyze the breaking and forming of bonds within a molecule.
  • Intramolecular Transferases: Transfer functional groups within a molecule.
  • Intramolecular Oxidoreductases: Catalyze oxidation-reduction reactions within a molecule .
Biological Properties

Isomerases exhibit diverse biological properties, including their expression patterns and tissue distribution. These enzymes are typically proteins with specific three-dimensional structures that enable their catalytic activity . The expression of isomerases can vary significantly across different tissues and developmental stages, reflecting their specialized roles in various biological processes. For example, glucose isomerase is widely distributed in bacteria, actinomycetes, fungi, and plants .

Biological Functions

Isomerases play crucial roles in numerous biological functions. They are involved in essential metabolic pathways such as glycolysis and carbohydrate metabolism . For instance, glucose isomerase catalyzes the reversible isomerization of D-glucose and D-xylose to D-fructose and D-xylulose, respectively . Additionally, isomerases can participate in immune responses and pathogen recognition by facilitating the structural rearrangement of molecules involved in these processes .

Modes of Action

The mechanisms of action of isomerases involve interactions with other molecules and cells. These enzymes typically bind to their substrates through specific active sites, facilitating the conversion of one isomer to another . For example, glucose isomerase binds to glucose and catalyzes its conversion to fructose through a series of intramolecular rearrangements . The downstream signaling cascades triggered by isomerase activity can vary depending on the specific enzyme and its role in the cell.

Regulatory Mechanisms

The expression and activity of isomerases are tightly regulated through various mechanisms. Transcriptional regulation involves the control of gene expression at the level of transcription, ensuring that isomerases are produced in response to specific cellular needs . Post-translational modifications, such as phosphorylation and glycosylation, can also modulate the activity and stability of isomerases . These regulatory mechanisms ensure that isomerases function optimally within the cellular environment.

Applications

Isomerases have numerous applications in biomedical research, diagnostic tools, and therapeutic strategies. For example, glucose isomerase is extensively used in the industrial production of high-fructose corn syrup and bioethanol . In biomedical research, isomerases are employed to study metabolic pathways and enzyme kinetics. Additionally, isomerases can serve as diagnostic markers for certain diseases and as targets for therapeutic interventions .

Role in the Life Cycle

Isomerases play vital roles throughout the life cycle, from development to aging and disease. During development, isomerases are involved in key metabolic processes that support growth and differentiation . In aging, the activity of isomerases can influence cellular metabolism and the maintenance of cellular homeostasis. Dysregulation of isomerase activity has been implicated in various diseases, including metabolic disorders and cancer .

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