CCNA2 Human

Cyclin-A2 Human Recombinant

This recombinant CCNA2 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 456 amino acids (residues 1-432), resulting in a molecular weight of 51.1 kDa. The protein includes a 24-amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2050
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized through filtration.

CCNB1 Human

Cyclin-B1 Human Recombinant

Recombinant human CCNB1, produced in E. coli, is a single polypeptide chain with 457 amino acids (1-433) and a molecular weight of 50.9 kDa. This protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2121
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

CCNB2 Human

Cyclin-B2 Human Recombinant

Recombinant human CCNB2, produced in E. coli, is a single polypeptide chain consisting of 422 amino acids (residues 1-398) with a molecular weight of 47.9 kDa. This protein features a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2200
Source
E.coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

CCND2 Human

Cyclin D2 Human Recombinant

Recombinant human CCND2, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 313 amino acids, encompassing residues 1 to 289 of the native CCND2 sequence, along with an N-terminal 24-amino acid His-tag for purification. The molecular weight of this recombinant CCND2 protein is 35.6 kDa. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2274
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

CCNG1 Human

Cyclin G1 Human Recombinant

Recombinant human CCNG1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 315 amino acids (specifically, amino acids 1 to 295). It has a molecular weight of 36.2 kDa. For purification purposes, a 20 amino acid His-tag is fused to the N-terminus of CCNG1, and purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2368
Source
Escherichia Coli.
Appearance
The product is a clear, colorless solution that has been sterilized by filtration.

CCNH Human

Cyclin-H Human Recombinant

Recombinant human CCNH, produced in E. coli, is a single, non-glycosylated polypeptide chain of 343 amino acids (1-323 a.a.) with a molecular weight of 39.8 kDa. It includes a 20 amino acid His Tag and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2457
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile filtered solution.

CCNI Human

Cyclin-I Human Recombinant

Recombinant human CCN1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 400 amino acids (residues 1-377) with a molecular weight of 44.9 kDa. It includes an N-terminal 23 amino acid His-tag and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2555
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Cyclins are a family of proteins that play a crucial role in regulating the cell cycle by activating cyclin-dependent kinases (CDKs). They were first discovered by R. Timothy Hunt in 1982 while studying the cell cycle of sea urchins . Cyclins are classified based on their behavior and function during different phases of the cell cycle. The main classes include:

  • G1 Cyclins: Present during the G1 phase, they help prepare the cell for DNA replication.
  • G1/S Cyclins: These cyclins appear at the transition from G1 to S phase and initiate DNA replication.
  • S Cyclins: Active during the S phase, they are involved in DNA synthesis.
  • M Cyclins: Present during mitosis, they help in the formation of the mitotic spindle and chromosome alignment .
Biological Properties

Cyclins exhibit distinct biological properties, including:

  • Expression Patterns: Cyclin levels oscillate throughout the cell cycle, with specific cyclins peaking at different phases .
  • Tissue Distribution: Cyclins are ubiquitously expressed in proliferating cells across various tissues. However, their expression levels can vary depending on the tissue type and the cell’s proliferative state .
Biological Functions

Cyclins primarily function by binding to and activating CDKs, which then phosphorylate target proteins to drive cell cycle progression . They play a role in:

  • Cell Cycle Regulation: Cyclins ensure the accurate transmission of genetic information by regulating the cell cycle checkpoints .
  • Immune Responses and Pathogen Recognition: Cyclins are involved in the regulation of immune cell proliferation and differentiation, which is crucial for effective immune responses .
Modes of Action

Cyclins exert their effects through several mechanisms:

  • Binding Partners: Cyclins bind to CDKs, forming active complexes that phosphorylate target proteins .
  • Downstream Signaling Cascades: The cyclin-CDK complexes activate various downstream signaling pathways that control cell cycle progression, DNA replication, and mitosis .
Regulatory Mechanisms

The expression and activity of cyclins are tightly regulated through:

  • Transcriptional Regulation: Cyclin gene expression is controlled by various transcription factors and signaling pathways .
  • Post-Translational Modifications: Cyclins undergo ubiquitination and subsequent degradation by the proteasome, ensuring their levels are appropriately regulated throughout the cell cycle .
Applications

Cyclins have several applications in biomedical research and clinical settings:

  • Diagnostic Tools: Cyclin levels can serve as biomarkers for cell proliferation and cancer diagnosis .
  • Therapeutic Strategies: Targeting cyclin-CDK complexes with specific inhibitors is a promising approach for cancer therapy .
Role in the Life Cycle

Cyclins play a vital role throughout the life cycle of an organism:

  • Development: Cyclins regulate cell division during embryonic development and tissue growth .
  • Aging and Disease: Dysregulation of cyclin expression and activity is associated with aging and various diseases, including cancer .
© Copyright 2024 Thebiotek. All Rights Reserved.