Product List

CA1 Human, Active

Carbonic Anhydrase-1 Human Recombinant, BioActive

CA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261) and having a molecular mass of 31.0 kDa.

CA1 Humanis fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT28835
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CA10 Human

Carbonic Anhydrase X Human Recombinant

CA10 Human Recombinant is a single, glycosylated polypeptide chain containing 317 amino acids (22-328a.a) and having a molecular mass of 36.3kDa (calculated). CA10 is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT28931
Source

HEK293 Cells.

Appearance
Sterile Filtered colorless solution.

CA11 Human

Carbonic Anhydrase XI Human Recombinant

CA11 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 326 amino acids (24-328) and having a molecular mass of 36.3kDa.
CA11 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29029
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CA12 Human

Carbonic Anhydrase XII Human Recombinant

CA12 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 283 amino acids (25-301a.a.) and having a molecular mass of 31.94kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).
CA12 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29055
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered clear solution.

CA13 Human

Carbonic Anhydrase XIII Human Recombinant

CA13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-262a.a) and having a molecular mass of 31.8kDa.
CA13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29120
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CA1 E.Coli

Carbonic Anhydrase-1 E.Coli Recombinant

CA1 E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 240 amino acids (1-220) and having a molecular mass of 27.0 kDa.

CA1 E.Coli is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT28665
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CA1 Human

Carbonic Anhydrase-1 Human Recombinant

Recombinant Human Carbonic anhydrase 1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a) and having a molecular mass of 31 kDa. Carbonic anhydrase 1 is fused to a 20 amino acids His-Tag at N-terminus and purified by conventional chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT28766
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

CA14 Human

Carbonic Anhydrase XIV Human Recombinant

CA14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (16-290 a.a.) and having a molecular mass of 33.2kDa.
CA14 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29180
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

CA3 Human

Carbonic Anhydrase III Human Recombinant

CA3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 260 amino acids (1-260 a.a.) and having a molecular mass of 29.5 kDa. The CA3 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29241
Source
Escherichia Coli.
Appearance
Sterile Filtered clear colorless solution.

CA8 Human

Carbonic Anhydrase 8 Human Recombinant

CA8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-290) and having a molecular mass of 35.5kDa.
CA8 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29323
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

Carbonic anhydrase (CA) is a metalloenzyme that catalyzes the reversible hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃⁻) and protons (H⁺) . This enzyme is crucial for maintaining acid-base balance and facilitating CO₂ transport in various organisms. CAs are classified into several distinct families based on their structure and evolutionary origin: α, β, γ, δ, ζ, η, and θ . The α-family is predominant in humans, while other families are found in plants, algae, and bacteria .

Biological Properties

Key Biological Properties: Carbonic anhydrase is characterized by its high catalytic efficiency and the presence of a zinc ion at its active site . This enzyme is involved in rapid interconversion between CO₂ and bicarbonate, which is essential for various physiological processes .

Expression Patterns and Tissue Distribution: CA is ubiquitously expressed in many tissues, including red blood cells, kidneys, lungs, and the gastrointestinal tract . Different isoforms of CA are distributed in specific tissues, reflecting their specialized functions. For example, CA II is abundant in red blood cells, while CA IV is found in the kidneys and lungs .

Biological Functions

Primary Biological Functions: The primary function of CA is to catalyze the reversible hydration of CO₂, which is vital for respiration and pH regulation . In the lungs, CA facilitates the conversion of CO₂ to bicarbonate, aiding in CO₂ transport from tissues to the lungs for exhalation .

Role in Immune Responses and Pathogen Recognition: CA plays a role in maintaining the pH balance in various tissues, which is crucial for immune cell function and pathogen recognition . By regulating the local pH, CA can influence the activity of immune cells and the effectiveness of the immune response .

Modes of Action

Mechanisms with Other Molecules and Cells: CA interacts with various molecules and cells to maintain acid-base homeostasis. It catalyzes the conversion of CO₂ to bicarbonate and protons, which are then transported across cell membranes .

Binding Partners and Downstream Signaling Cascades: CA binds to zinc ions at its active site, which is essential for its catalytic activity . The enzyme’s activity can influence downstream signaling pathways involved in pH regulation and ion transport .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CA are regulated at multiple levels, including transcriptional regulation and post-translational modifications . Various factors, such as pH, CO₂ levels, and hormonal signals, can influence CA expression .

Transcriptional Regulation and Post-Translational Modifications: Transcription factors and signaling molecules can modulate the expression of CA genes . Post-translational modifications, such as phosphorylation and acetylation, can also affect the enzyme’s activity and stability .

Applications

Biomedical Research: CA is extensively studied in biomedical research due to its role in various physiological processes and diseases . Researchers are exploring CA inhibitors as potential therapeutic agents for conditions like glaucoma, epilepsy, and cancer .

Diagnostic Tools and Therapeutic Strategies: CA inhibitors, such as acetazolamide, are used to treat glaucoma by reducing intraocular pressure . Additionally, CA is being investigated for its potential in cancer therapy, as certain isoforms are overexpressed in tumors .

Role in the Life Cycle

Role Throughout the Life Cycle: CA plays a critical role throughout the life cycle, from development to aging and disease . During development, CA is involved in processes like bone formation and neural development . In aging, changes in CA activity can affect physiological functions and contribute to age-related diseases .

From Development to Aging and Disease: CA’s role in maintaining pH balance and facilitating CO₂ transport is essential for various stages of life . Dysregulation of CA activity can lead to conditions like osteoporosis, neurodegenerative diseases, and metabolic disorders .

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