CDK1 Human, Sf9

Cyclin-Dependent Kinase 1 Human Recombinant, Sf9

CDK1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 35.1 kDa. It comprises 305 amino acids (1-297a.a.). CDK1 is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT589
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless solution, sterile-filtered.

CDK16 Human

Cyclin-dependent kinase 16 Human Recombinant

Recombinant human CDK16, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 362 amino acids (residues 158-496). This protein, with a molecular weight of 41.1 kDa, is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT641
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

p16-INK4a Human

Cyclin-Dependent Kinase Inhibitor 2A Human Recombinant

Recombinant human CDKN2A, a 16.5 kDa protein, is produced in E. coli as a single, non-glycosylated polypeptide chain comprising 156 amino acids. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1908
Source
Escherichia Coli.
Appearance
White, lyophilized powder, sterile-filtered.

p16-INK4a Human, TAT

Cyclin-Dependent Kinase Inhibitor 2A Human Recombinant, TAT

p16-INK4a Human Recombinant is a non-glycosylated polypeptide chain produced in E. coli bacteria. It contains 168 amino acids, including the full 156 amino acids of the p16-INK4a protein and a 13-amino acid TAT peptide tag (GGYGRKKRRQRRR). The total molecular weight of the recombinant protein is 18 kDa. Purification is achieved using specialized chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1971
Source
Escherichia Coli.
Appearance
The product is a sterile, white powder that has been freeze-dried.

CDK3 Human

Cyclin-Dependent Kinase 3 Human Recombinant

Recombinant human CDK3, produced in E. coli, is a single polypeptide chain consisting of 328 amino acids (residues 1-305) with a molecular weight of 37.4 kDa. It includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1188
Source
Escherichia Coli.
Appearance
A sterile, colorless solution that has been filtered.

CDK-4 Human

Cyclin-Dependent Kinase 4 Human Recombinant

This product consists of recombinant human Cyclin-Dependent Kinase 4, expressed in E. coli. This full-length protein has a molecular weight of 38 kDa and includes an amino-terminal hexahistidine tag. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1270
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

CDK5 Human

Cyclin-dependent Kinase 5 Human Recombinant

Recombinant human CDK5, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 316 amino acids (residues 1-292), resulting in a molecular weight of 35.8 kDa. This CDK5 protein has a 24 amino acid His-tag fused at its N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1361
Source
Escherichia Coli.
Appearance
A clear, colorless solution that is sterile and filtered.

CDK5 Human, Sf9

Cyclin-Dependent Kinase 5 Human Recombinant,Sf9

CDK5, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain consisting of 298 amino acids (1-292a.a.) with a molecular weight of 34.1 kDa. It's important to note that the molecular size observed on SDS-PAGE will appear between 28-40 kDa. This CDK5 protein is engineered with a 6-amino acid Histidine tag at the C-terminus and purified through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1443
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

CDK1 Human

Cyclin-Dependent Kinase 1 Human Recombinant

Recombinant human CDK1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 317 amino acids (amino acids 1-297) with a molecular weight of 36.2 kDa. A 20 amino acid His-tag is fused to the N-terminus of the CDK1 protein. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT506
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

CDKN1A Human

Cyclin-Dependent Kinase Inhibitor 1A Human Recombinant

Recombinant human CDKN1A, produced in E. coli, is a single polypeptide chain comprising 184 amino acids (residues 1-164) with a molecular weight of 20.2 kDa. A 20 amino acid His-tag is fused to the N-terminus of CDKN1A, and the protein is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT1496
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.
Definition and Classification

Cyclin-Dependent Kinases (CDKs) are a family of protein kinases that play crucial roles in regulating the cell cycle. They are named for their dependency on cyclins, a group of proteins that bind to CDKs, activating them at specific points in the cell cycle. CDKs are classified based on their functions and the cyclins they interact with. The main classes include:

  • Cell Cycle CDKs: These include CDK1, CDK2, CDK4, and CDK6, which are primarily involved in cell cycle regulation.
  • Transcriptional CDKs: These include CDK7, CDK8, CDK9, CDK12, and CDK13, which are involved in regulating transcription.
Biological Properties

Key Biological Properties: CDKs are serine/threonine kinases that phosphorylate target proteins, leading to changes in their activity. They are highly conserved across eukaryotic species.

Expression Patterns: CDKs are ubiquitously expressed in proliferating cells. Their expression levels can vary depending on the cell type and the phase of the cell cycle.

Tissue Distribution: CDKs are found in various tissues, with higher expression in tissues with high proliferative capacity, such as the bone marrow, skin, and gastrointestinal tract.

Biological Functions

Primary Biological Functions: CDKs regulate the progression of the cell cycle by phosphorylating key substrates involved in DNA replication, mitosis, and cell division. They ensure that the cell cycle progresses in an orderly and timely manner.

Role in Immune Responses: CDKs are involved in the proliferation of immune cells, such as T and B lymphocytes, during immune responses.

Pathogen Recognition: CDKs can influence the immune system’s ability to recognize and respond to pathogens by regulating the proliferation and differentiation of immune cells.

Modes of Action

Mechanisms with Other Molecules and Cells: CDKs interact with cyclins to form active complexes. These complexes phosphorylate target proteins, leading to changes in their activity and function.

Binding Partners: CDKs bind to specific cyclins, which determine their substrate specificity and activity. For example, CDK1 binds to cyclin B to regulate mitosis, while CDK2 binds to cyclin E to regulate the G1/S transition.

Downstream Signaling Cascades: CDKs activate downstream signaling pathways that control various cellular processes, such as DNA replication, mitosis, and cell division.

Regulatory Mechanisms

Regulatory Mechanisms: CDK activity is tightly regulated by various mechanisms to ensure proper cell cycle progression.

Transcriptional Regulation: The expression of CDKs and cyclins is regulated at the transcriptional level by various transcription factors and signaling pathways.

Post-Translational Modifications: CDKs are regulated by post-translational modifications, such as phosphorylation and ubiquitination, which can activate or inhibit their activity.

Applications

Biomedical Research: CDKs are studied extensively in biomedical research to understand their roles in cell cycle regulation and their implications in diseases such as cancer.

Diagnostic Tools: CDK activity and expression levels can serve as biomarkers for certain cancers and other proliferative disorders.

Therapeutic Strategies: CDK inhibitors are being developed as potential therapeutic agents for treating cancers and other diseases characterized by uncontrolled cell proliferation.

Role in the Life Cycle

Role Throughout the Life Cycle: CDKs play essential roles throughout the life cycle, from development to aging and disease.

Development: CDKs are crucial for the proper development of tissues and organs by regulating cell proliferation and differentiation.

Aging: CDK activity can decline with age, leading to reduced cell proliferation and tissue regeneration.

Disease: Dysregulation of CDK activity is associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.

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