ACE2 (18-740) Human, Fc

Angiotensin Converting Enzyme 2 (18-740 a.a.), Fc Human Recombinant

This recombinant ACE2 protein is produced in HEK293 cells and encompasses amino acids 18-740 of the human ACE2 protein. It also includes a C-terminal Fc tag. This ACE2 protein demonstrates binding affinity for the Spike receptor binding domain of the SARS Coronavirus-2 [CoV-2019].

Shipped with Ice Packs
Cat. No.
BT24301
Source

HEK293 Cells

Appearance
A clear, sterile-filtered solution.

ACE2 (19-740) Human

Angiotensin Converting Enzyme 2 (19-740 a.a.), Human Recombinant

This recombinant human ACE2 protein, derived from CHO cells, consists of the extracellular domain (amino acids 19-740) fused to an Fc tag at the C-terminus. It binds to the receptor-binding domain of the SARS-CoV-2 spike protein.

Shipped with Ice Packs
Cat. No.
BT24507
Source

CHO Cells

Appearance
A clear, sterile-filtered solution.

ACE2 Mouse

Angiotensin Converting Enzyme 2 Mouse Recombinant

ACE2 Mouse, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It comprises 731 amino acids (specifically, amino acids 18-740) and has a molecular weight of 84.5 kDa. The protein includes a 6-amino acid Histidine tag (His-Tag) attached to its C-terminus to facilitate purification. It is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT24590
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

ACE2 (18-615) Human

Angiotensin Converting Enzyme 2 (18-615 a.a.), Human Recombinant

This recombinant ACE2 protein is derived from Chinese hamster ovary (CHO) cells. It contains the extracellular domain of human ACE2 (amino acids 18-615) fused to an Fc tag at the C-terminus. This protein has a molecular weight of approximately 130 kDa and binds to the receptor binding domain of the SARS-CoV-2 spike protein.

Shipped with Ice Packs
Cat. No.
BT24017
Source

CHO Cells

Appearance
Clear solution, sterile-filtered.

ACE2 (18-740) Human

Angiotensin Converting Enzyme 2 (18-740 a.a.), Human Recombinant

This recombinant ACE2 protein is produced in HEK293 cells and corresponds to the human ACE2 protein sequence from amino acids 18 to 740. It is fused to an IgG-His tag at the N-terminus, adding 242 amino acids and resulting in a total molecular weight of 110.8 kDa. This ACE2 protein specifically binds to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein.

Shipped with Ice Packs
Cat. No.
BT24137
Source

HEK293 Cells

Appearance
Clear solution, sterile-filtered.

ACE2 (18-740) Human, Biotin

Angiotensin Converting Enzyme 2 (18-740 a.a.), Biotinylated Human Recombinant

This recombinant ACE2 protein is produced in HEK293 cells and corresponds to amino acids Gln 18 to Ser 740 of the human ACE2 protein. It is biotinylated and has a His-AVI tag at the C-terminus. The predicted molecular weight is 87.2 kDa, but it migrates at 95-125 kDa on SDS-PAGE under reducing conditions due to glycosylation. This protein binds to the receptor-binding domain of the SARS-CoV-2 spike protein.

Shipped with Ice Packs
Cat. No.
BT24216
Source

HEK293 Cells

Appearance
Clear, sterile-filtered solution.

ACE2 Rat

Angiotensin Converting Enzyme 2 Rat Recombinant

ACE2 Rat, produced using Sf9 insect cells and Baculovirus expression system, is a single, glycosylated polypeptide chain. It consists of 731 amino acids (specifically, amino acids 18 to 740), resulting in a molecular weight of 84.7kDa. The ACE2 protein has an 8 amino acid Histidine tag (His-Tag) added to its C-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT24722
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Angiotensin-converting enzyme 2 (ACE2) is a zinc-containing metalloenzyme that plays a crucial role in the renin-angiotensin-aldosterone system (RAAS), which regulates blood pressure and fluid balance . ACE2 can be found either attached to the membrane of cells (mACE2) or in a soluble form (sACE2) . It is classified as a carboxypeptidase and is a homolog of angiotensin-converting enzyme (ACE) .

Biological Properties

Key Biological Properties: ACE2 acts as a peptidase, catalyzing the cleavage of angiotensin II (Ang II) into angiotensin (1-7), which has vasodilatory effects .

Expression Patterns and Tissue Distribution: ACE2 is primarily expressed in the heart, kidneys, lungs, testes, intestines, and adipose tissue . It is also found in the intestines, kidney, testis, gallbladder, and heart .

Biological Functions

Primary Biological Functions: ACE2 counterbalances the effects of ACE by converting Ang II, a vasoconstrictor, into Ang (1-7), a vasodilator . This helps in lowering blood pressure and protecting organs from inflammatory injuries .

Role in Immune Responses and Pathogen Recognition: ACE2 serves as a functional receptor for coronaviruses, including SARS-CoV and SARS-CoV-2, facilitating viral entry into host cells . This makes it a critical player in immune responses and pathogen recognition.

Modes of Action

Mechanisms with Other Molecules and Cells: ACE2 interacts with the spike protein of SARS-CoV-2, allowing the virus to enter and infect cells . It also binds to MasR receptors, creating localized vasodilation and decreasing blood pressure .

Binding Partners and Downstream Signaling Cascades: ACE2’s enzymatic activity leads to the production of Ang (1-7), which binds to MasR receptors, initiating signaling cascades that result in vasodilation and anti-inflammatory effects .

Regulatory Mechanisms

Transcriptional Regulation: ACE2 expression is regulated at multiple levels, including transcriptional and post-transcriptional mechanisms .

Post-Translational Modifications: ACE2 undergoes glycosylation, phosphorylation, and shedding from the plasma membrane, which modulates its activity .

Applications

Biomedical Research: ACE2 is extensively studied for its role in cardiovascular diseases and as a receptor for SARS-CoV-2 .

Diagnostic Tools: ACE2 levels can be used as biomarkers for various diseases, including COVID-19 .

Therapeutic Strategies: Targeting ACE2 with inhibitors or activators is being explored for treating cardiovascular diseases and COVID-19 .

Role in the Life Cycle

Development to Aging and Disease: ACE2 plays a vital role throughout the life cycle, from development to aging. It helps maintain cardiovascular health and protect organs from damage . In diseases like COVID-19, ACE2’s role as a viral receptor makes it a critical factor in disease progression and severity .

ACE2’s multifaceted roles in physiology and disease make it a significant focus of ongoing research, with potential applications in diagnostics and therapeutics.

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