NEIL2 Human

Nei Endonuclease VIII-Like 2 Human Recombinant

Recombinant human NEIL2, produced in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 356 amino acids (1-332), with a molecular weight of 39.4kDa. The protein includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT16179
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

NTH E.Coli

Endonuclease-III E.Coli Recombinant

Recombinant NTH from E. coli, fused with a 20 amino acid His tag at the N-terminus, is produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 231 amino acids (1-211a.a.) with a molecular weight of 25.7 kDa. The purification of NTH is carried out using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16269
Source
Escherichia Coli.
Appearance
A sterile, filtered solution that is colorless.

NTHL1 Human

Nth Endonuclease III-Like 1 Human Recombinant

Recombinant human NTHL1 is produced in E. coli as a single, non-glycosylated polypeptide chain. It consists of 332 amino acids, with amino acids 1-312 representing the NTHL1 sequence, and a 20 amino acid His-tag fused at the N-terminus. The protein has a molecular weight of 36.6 kDa and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16346
Source
Escherichia Coli.
Appearance
Clear and colorless solution, sterile-filtered.

TSEN15 Human

tRNA Splicing Endonuclease 15 Human Recombinant

Recombinant Human TSEN15, expressed in E. coli, is a purified protein with a His tag (20 amino acids) attached to its N-terminus. This single, non-glycosylated polypeptide chain is 191 amino acids long (including amino acids 1-171) with a molecular weight of 20.9 kDa. The protein purification process utilizes proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT16434
Source
Escherichia Coli.
Appearance
A sterile, colorless solution, free from particulate matter.

FEN1 Human

Flap Structure-Specific Endonuclease 1 Human Recombinant

Recombinant Human FEN1, expressed in E. coli, is a purified, non-glycosylated polypeptide chain consisting of 380 amino acids (1-380 a.a.). With a molecular weight of 42.5 kDa, this protein is purified using standardized chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT16013
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

NEIL1 Human

Nei Endonuclease VIII-Like 1 Human Recombinant

Recombinantly produced in E.coli, NEIL1 Human Recombinant is a single, non-glycosylated polypeptide chain. This 45.8kDa protein comprises 410 amino acids (1-390) and features a 20 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16093
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, either DNA or RNA . They are distinct from exonucleases, which cleave nucleotides from the ends of the chain . Endonucleases can be classified into several types based on their specificity and mechanism of action:

  • Type I: Large, multisubunit complexes that cleave DNA at random sites far from their recognition sequences and require ATP .
  • Type II: Simpler enzymes that cleave DNA at specific sites within or near their recognition sequences without requiring ATP .
  • Type III: Multisubunit complexes that cleave DNA at specific sites close to their recognition sequences and require ATP .
Biological Properties

Endonucleases exhibit several key biological properties:

  • Expression Patterns: Endonucleases are expressed in various organisms, including bacteria, archaea, and eukaryotes .
  • Tissue Distribution: In eukaryotes, endonucleases are distributed across different tissues, playing roles in DNA repair, replication, and transcription .
  • Key Biological Properties: They are crucial for maintaining genomic integrity by repairing damaged DNA and processing RNA molecules .
Biological Functions

Endonucleases perform several primary biological functions:

  • DNA Repair: They play a critical role in repairing damaged DNA by cleaving damaged or mismatched regions .
  • RNA Processing: Endoribonucleases are involved in the precise cleavage of precursor RNA molecules to generate functional RNAs .
  • Immune Responses: Some endonucleases, such as restriction enzymes, protect bacteria from viral infections by degrading foreign DNA .
  • Pathogen Recognition: They help recognize and eliminate pathogenic DNA or RNA within cells .
Modes of Action

Endonucleases interact with other molecules and cells through various mechanisms:

  • Binding Partners: They often bind to specific DNA or RNA sequences, forming complexes with other proteins to facilitate cleavage .
  • Downstream Signaling Cascades: The cleavage of DNA or RNA by endonucleases can trigger downstream signaling pathways involved in DNA repair, apoptosis, or immune responses .
Regulatory Mechanisms

The expression and activity of endonucleases are tightly regulated:

  • Transcriptional Regulation: Gene expression of endonucleases is controlled by transcription factors that respond to cellular stress or damage .
  • Post-Translational Modifications: Endonucleases undergo various post-translational modifications, such as phosphorylation, to modulate their activity and stability .
Applications

Endonucleases have numerous applications in biomedical research and clinical settings:

  • Biomedical Research: They are used in gene editing technologies, such as CRISPR-Cas9, to create targeted DNA modifications .
  • Diagnostic Tools: Endonucleases are employed in molecular diagnostics to detect specific DNA or RNA sequences .
  • Therapeutic Strategies: They are explored as potential therapeutic agents for treating genetic disorders and cancers by targeting specific DNA sequences .
Role in the Life Cycle

Endonucleases play vital roles throughout the life cycle of organisms:

  • Development: They are involved in DNA replication and repair during cell division and development .
  • Aging: Endonucleases help maintain genomic stability, which is crucial for preventing age-related diseases .
  • Disease: Dysregulation of endonuclease activity can lead to various diseases, including cancer and neurodegenerative disorders .
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