FKBP14 Human

FK506 Binding Protein 14 Human Recombinant

Recombinant FKBP14, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 213 amino acids (with the sequence spanning from amino acid 20 to 211) and has a molecular weight of 24.2 kDa. For purification purposes, a 21 amino acid His-Tag is fused to the N-terminus of the FKBP14 protein. The purification process employs proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20056
Source
Escherichia Coli.
Appearance
The product is a clear, colorless solution that has been sterilized by filtration.

FKBP1A Human

FK506 Binding Protein 1A Human Recombinant

Recombinant human FKBP1A, with an N-terminal His-Tag, was produced in E. coli. This protein is a single, non-glycosylated polypeptide chain purified using Ni2+-affinity chromatography, followed by gel filtration.
Shipped with Ice Packs
Cat. No.
BT20143
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

FKBP1A Mouse

FK506 Binding Protein 1A Mouse Recombinant

Recombinant FKBP1A from mouse has been expressed in E. coli. It is a single, non-glycosylated polypeptide chain containing 132 amino acids (residues 1-108), possessing a His-tag on the N-terminus, and having a molecular weight of 14.4 kDa. The protein was purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20234
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.

FKBP1B Human

FK506 Binding Protein 1B Human Recombinant

Recombinant human FKBP1B, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein comprises 130 amino acids, spanning residues 1 to 108, with a molecular weight of 14.2 kDa. For purification purposes, FKBP1B is fused to a 22-amino acid His-tag at the N-terminus and subsequently purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20346
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterilized by filtration.

FKBP2 Human

FK506 Binding Protein 2 Human Recombinant

Recombinant human FKBP2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 122 amino acids (residues 22-142) and has a molecular weight of 13.4 kDa. The purification of FKBP2 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20433
Source
Escherichia Coli.
Appearance
The product is a sterile-filtered solution, clear and colorless in appearance.

FKBP3 Human

FK506 Binding Protein 3 Human Recombinant

Produced in E.Coli, FKBP3 is a single, non-glycosylated polypeptide chain consisting of 224 amino acids (amino acids 1-224). Its molecular weight is 25.1 kDa. The purification of FKBP3 is carried out using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20524
Source
Escherichia Coli.
Appearance
A colorless solution that has been sterilized by filtration.

FKBP4 Human

FK506 Binding Protein 4 Human Recombinant

Produced in E. coli, this FKBP4 protein is a single, non-glycosylated polypeptide chain comprising 479 amino acids (1-459 a.a.) with a molecular weight of 53.9 kDa. A 20 amino acid His Tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20597
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

FKBP5 Human

FK506 Binding Protein 5 Human Recombinant

Recombinant human FKBP5 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 52.5kDa. The protein sequence consists of 467 amino acids, including a 10 amino acid His tag located at the N-terminus (Met1-Val457).
Shipped with Ice Packs
Cat. No.
BT20660
Source
Escherichia Coli.
Appearance
White powder, lyophilized (freeze-dried) and filtered.

FKBP6 Human

FK506 Binding Protein 6 Human Recombinant

Recombinant human FKBP6, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 347 amino acids (specifically, residues 1-327). This protein exhibits a molecular weight of 39.3 kDa. For purification purposes, a 20-amino acid His tag is fused to the N-terminus of the FKBP6 protein, and standard chromatography techniques are employed.
Shipped with Ice Packs
Cat. No.
BT20769
Appearance
A clear, colorless solution that has been sterilized by filtration.

FKBPL Human

FK506 Binding Protein Like Human Recombinant

Recombinant Human FKBPL, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 357 amino acids (residues 1-349) with a molecular weight of 39.2 kDa. An 8 amino acid His-Tag is fused to the C-terminus of FKBPL, and the protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20890
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.
Definition and Classification

FK506 Binding Proteins (FKBPs) are a family of proteins that possess peptidyl-prolyl cis-trans isomerase activity, facilitating the folding of proteins containing proline residues . They are classified as immunophilins due to their ability to bind immunosuppressive drugs such as FK506 (tacrolimus) and rapamycin . FKBPs are found in a wide range of organisms, from yeast to humans .

Biological Properties

Key Biological Properties: FKBPs exhibit peptidyl-prolyl isomerase activity, which is crucial for protein folding and function . They also play roles in various cellular processes, including signal transduction and protein trafficking .

Expression Patterns and Tissue Distribution: FKBPs are ubiquitously expressed in various tissues. For instance, FKBP12 is abundantly present in the cytoplasm and nucleus of cells . FKBP25, another member of the family, is primarily found in the nucleus and is involved in DNA repair .

Biological Functions

Primary Biological Functions: FKBPs are involved in protein folding, cellular signaling, apoptosis, and transcription . They act as molecular switches by binding and altering the conformation of their target proteins .

Role in Immune Responses and Pathogen Recognition: FKBP12, in particular, binds to the immunosuppressant molecule tacrolimus, which inhibits the activity of calcineurin, a phosphatase involved in T-cell activation . This inhibition suppresses the immune response, making FKBP12 crucial in preventing organ rejection in transplant patients .

Modes of Action

Mechanisms with Other Molecules and Cells: FK506 (tacrolimus) binds to FKBP12, forming a complex that inhibits calcineurin . This inhibition prevents the dephosphorylation and activation of nuclear factor of activated T-cells (NFAT), thereby suppressing the transcription of interleukin-2 (IL-2) and other cytokines .

Binding Partners and Downstream Signaling Cascades: The FKBP12-tacrolimus complex inhibits the calcineurin-dependent NFAT pathway and the calcineurin-independent activation pathway for JNK and p38 . This dual inhibition is critical for the immunosuppressive effects of tacrolimus .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of FKBP genes is regulated by various transcription factors and signaling pathways . For example, the fkbR and fkbN genes positively regulate FK506 biosynthesis in Streptomyces tsukubaensis .

Transcriptional Regulation and Post-Translational Modifications: FKBP expression is controlled at the transcriptional level by regulatory elements within their gene clusters . Post-translational modifications, such as phosphorylation, also play a role in modulating FKBP activity .

Applications

Biomedical Research: FKBPs are used as tools for chemically induced dimerization, which allows researchers to manipulate protein localization, signaling pathways, and protein activation .

Diagnostic Tools and Therapeutic Strategies: FKBP12 is targeted by immunosuppressive drugs like tacrolimus, which are used to prevent organ rejection in transplant patients and treat autoimmune disorders . Additionally, FKBP inhibitors are being explored for their potential in cancer therapy .

Role in the Life Cycle

Role Throughout the Life Cycle: FKBPs are involved in various stages of development and aging. They participate in signal transduction, hormonal pathways, stress responses, and gene transcription . Mutations or dysregulation of FKBP genes can lead to developmental abnormalities and diseases .

From Development to Aging and Disease: FKBP12 regulates fundamental aspects of cell biology, including TGF-β receptor signaling, which is crucial for development and tissue homeostasis . Dysregulation of FKBP12 has been associated with diseases such as Alzheimer’s and cardiovascular diseases .

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