ACOT11 Human

Acyl-CoA Thioesterase 11 Human Recombinant

Recombinant human ACOT11, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising the topological domain (amino acids 19-250) and a 36-amino acid His-tag at the N-terminus. The protein has a molecular weight of 29.9 kDa.
Shipped with Ice Packs
Cat. No.
BT17913
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

ACOT13 Human

Acyl-CoA Thioesterase 13 Human Recombinant

Recombinant human ACOT13, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 160 amino acids, including a 20 amino acid His tag at the N-terminus, spanning amino acids 1-140. The protein has a molecular mass of 17.1 kDa. Purification of ACOT13 is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT17994
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

ACOT7 Human

Acyl-CoA Thioesterase 7 Human Recombinant

Recombinant human ACOT7, expressed in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 390 amino acids (residues 1-370) and has a molecular weight of 42.6kDa. For purification, a 20 amino acid His-tag is fused to the N-terminus, and proprietary chromatographic techniques are employed.
Shipped with Ice Packs
Cat. No.
BT18089
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless, and clear solution.

ACOT8 Human

Acyl-CoA Thioesterase 8 Human Recombinant

Recombinant human ACOT8, expressed in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 342 amino acids (amino acids 1-319) and has a molecular weight of 38.3kDa. The protein includes a 23 amino acid His-tag attached to the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT18184
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

CES1D Mouse

Carboxylesterase 1D Mouse Recombinant

Recombinant Mouse CES1D, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 555 amino acids (19-565 a.a), has a molecular mass of 60.9kDa, and appears at approximately 50-70kDa on SDS-PAGE due to glycosylation. The protein is purified using proprietary chromatographic techniques and includes an 8 amino acid His-tag at the C-terminus.
Shipped with Ice Packs
Cat. No.
BT18268
Source
Sf9, Baculovirus cells.
Appearance
Sterile, colorless solution.

CES1G Mouse

Carboxylesterase 1G Mouse Recombinant

CES1G, produced using Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 61.9kDa (observed as 50-70kDa on SDS-PAGE under reducing conditions). It consists of 556 amino acids (19-565 a.a.). The protein is expressed with a 6 amino acid His tag at the C-terminus and purified through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18340
Source
Sf9, Baculovirus cells.
Appearance
The product is a sterile, filtered solution that appears colorless.

Esterase D Human

Esterase D,S-Formylglutathione Hydrolase Human Recombinant

Recombinant Human Esterase-D, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein, with a molecular weight of 33.6 kDa, comprises 302 amino acids, including a 20 amino acid His tag at the N-terminus (1-282 a.a. of the target protein). Purification of Esterase-D is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18428
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

PDE6D Human

Phosphodiesterase 6D cGMP-Specific Rod Delta Human Recombinant

This product consists of PDE6D protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 158 amino acids (amino acids 1-150), resulting in a molecular weight of 18.4 kDa. The PDE6D is tagged at the C-terminus with an 8 amino acid His tag and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18486
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

PDE6H Human

Phosphodiesterase 6H cGMP-Specific Cone Gamma Human Recombinant

Recombinant PDE6H Human, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 106 amino acids (with amino acids 1-83 being part of the PDE6H sequence) and has a molecular mass of 11.5 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18545
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

PPME1 Human

Protein Phosphatase Methylesterase 1 Human Recombinant

This product is a laboratory-grade protein called PPME1. It is produced in bacteria and purified to a high degree. This protein is a single chain of 406 amino acids, with a molecular weight of 44.4 kDa. It contains a His-tag for easy purification.
Shipped with Ice Packs
Cat. No.
BT18599
Source
E.coli.
Appearance
Clear and colorless solution that has been sterilized by filtration.
Definition and Classification

Esterases are a class of hydrolase enzymes that catalyze the hydrolysis of ester bonds, converting esters into an acid and an alcohol through a reaction with water . They are classified based on their substrate specificity and protein structure. The main types include:

  • Carboxylic ester hydrolases: e.g., acetylesterase, cholinesterase, pectinesterase.
  • Thiolester hydrolases: e.g., thioesterase.
  • Phosphoric monoester hydrolases: e.g., phosphatase.
  • Phosphoric diester hydrolases.
  • Sulfuric ester hydrolases: e.g., sulfatases .
Biological Properties

Esterases exhibit diverse biological properties, including broad substrate specificity and stability under various conditions. They are expressed in multiple tissues, including the liver, blood plasma, and various microbial environments . Their tissue distribution is extensive, with significant roles in lipid metabolism and detoxification processes .

Biological Functions

Esterases play crucial roles in various biological functions:

  • Lipid Metabolism: They hydrolyze lipid esters, aiding in lipid absorption and metabolism .
  • Detoxification: Esterases help in the breakdown of xenobiotic compounds, contributing to detoxification .
  • Immune Responses: Certain esterases are involved in pathogen recognition and immune responses .
Modes of Action

Esterases interact with other molecules and cells through specific binding partners and downstream signaling cascades. The catalytic mechanism typically involves a serine residue in the active site, which attacks the carbonyl carbon of the ester bond, facilitated by histidine and aspartic/glutamic acid residues . This leads to the formation of an acyl-enzyme intermediate, which is subsequently hydrolyzed to release the products .

Regulatory Mechanisms

The expression and activity of esterases are regulated through various mechanisms:

  • Transcriptional Regulation: Gene expression is controlled by transcription factors and regulatory elements in the promoter regions .
  • Post-Translational Modifications: Esterase activity can be modulated by phosphorylation, glycosylation, and other modifications .
  • Environmental Factors: pH, temperature, and the presence of inhibitors or activators can influence esterase activity .
Applications

Esterases have numerous applications in biomedical research, diagnostics, and therapeutics:

  • Biomedical Research: Used as tools to study lipid metabolism and enzyme kinetics .
  • Diagnostic Tools: Employed in assays to detect specific ester substrates or products .
  • Therapeutic Strategies: Targeted for drug development, particularly in the treatment of metabolic disorders and detoxification therapies .
Role in the Life Cycle

Throughout the life cycle, esterases play vital roles from development to aging and disease:

  • Development: Involved in the regulation of lipid metabolism during growth and development .
  • Aging: Changes in esterase activity are associated with aging processes and age-related diseases .
  • Disease: Altered esterase activity is linked to various diseases, including metabolic disorders, neurodegenerative diseases, and cancer .
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