AICDA Human

Activation-Induced Cytidine Deaminase Human Recombinant

Recombinant human AICDA, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 218 amino acids (residues 1-198) with a molecular weight of 26.1 kDa. It includes an N-terminal 20 amino acid His-tag for purification purposes. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4705
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

AMPD2 Human

AMPD2 Human Recombinant

Recombinant human AMPD2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 667 amino acids (specifically, residues 236-879). It possesses a molecular mass of 77.0 kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4803
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

CDA Human

Cytidine Deaminase Human Recombinant

This product is a recombinant human CDA protein engineered with a 20 amino acid His tag at its N-terminus. It is produced in E. coli and purified to a single, non-glycosylated polypeptide chain of 166 amino acids (specifically amino acids 1-146). With a molecular weight of 18.3kDa, this protein is provided in a solution at a concentration of 0.5mg/ml and has been purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4882
Source
Escherichia Coli.
Appearance
A clear and colorless solution that has been sterilized by filtration.

DCTD Human

dCMP Deaminase Human Recombinant

This product consists of the recombinant human DCTD protein, expressed in E. coli and purified to a high degree. The protein is a single, non-glycosylated polypeptide chain with a molecular weight of 22.1 kDa. It encompasses amino acids 1 to 178 of the native DCTD sequence and includes an N-terminal His-tag (20 amino acids) to facilitate purification. The protein has been purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4936
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterilized by filtration.

FTCD Human

Formiminotransferase Cyclodeaminase Human Recombinant

Recombinant Formiminotransferase Cyclodeaminase (FTCD), also known as liver cytosol type 1, is produced using Spodoptera frugiperda (SF9) insect cells. This recombinant protein is glycosylated and has a molecular weight of 59,749 Daltons. For purification and detection purposes, the FTCD is expressed with a -6xHis tag, facilitating downstream purification by affinity chromatography techniques.
Shipped with Ice Packs
Cat. No.
BT5025
Source
Sf9 insect cells.

SDSL Human

Serine Dehydratase-Like Human Recombinant

Recombinant human SDSL, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 353 amino acids (residues 1-329) with a molecular weight of 37.3 kDa. This protein is engineered with a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5406
Source
E.coli.
Appearance
Clear, colorless solution, sterile-filtered.

ADA Human

Adenosine Deaminase Human Recombinant

This product consists of the recombinant human ADA enzyme, produced in E. coli bacteria. It is engineered as a single polypeptide chain without glycosylation, containing 383 amino acids (including a 20 amino acid His-Tag at the N-terminus) and possessing a molecular weight of 42.9 kDa. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT4378
Source
Escherichia Coli.
Appearance
The ADA protein is provided as a clear solution that has undergone sterile filtration.

ADAL Human

Adenosine Deaminase-Like Human Recombinant

This product contains a lab-made version of the human ADAL protein. It is produced in E. coli bacteria and purified to a high degree. This specific ADAL protein is a single chain of 292 amino acids, with an added 'His-tag' of 25 amino acids at one end. It has a molecular weight of 32.7kDa and is not glycosylated.
Shipped with Ice Packs
Cat. No.
BT4461
Source
Escherichia Coli.
Appearance
Clear and colorless liquid, free from any particles or cloudiness.

ADAT1 Human

Adenosine Deaminase tRNA-Specific 1 Human Recombinant

Recombinant Human Adenosine Deaminase tRNA-Specific 1 is a non-glycosylated polypeptide chain with a molecular weight of 57.7 kDa that is generated in E. Coli. It has the amino acids 1-502. A T7 tag is present at the N-terminus of ADAT-1. Using exclusive chromatographic methods, ADAT1 is purified.
Shipped with Ice Packs
Cat. No.
BT4570
Source
Escherichia Coli.
Appearance
A clear, sterile filtered solution.

ADAT2 Human

Adenosine Deaminase, tRNA-specific 2 Human Recombinant

Recombinant Human ADAT2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 211 amino acids (1-191 a.a.) with a molecular weight of 23.2 kDa. It includes a 20 amino acid His Tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4624
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterile filtered.
Definition and Classification

Deaminases are enzymes that catalyze the removal of an amino group from amino compounds, such as amino acids, through hydrolysis . They belong to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds . Deaminases can be classified based on their substrate specificity, such as adenosine deaminase (ADA), cytidine deaminase, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase .

Biological Properties

Deaminases exhibit diverse biological properties depending on their type and source. For instance, ACC deaminase is widespread in various bacterial and fungal species and is inducible in the presence of its substrate ACC . Adenosine deaminase (ADA) is crucial for purine metabolism and is expressed in various tissues, including the immune system . The tissue distribution of deaminases varies, with some being ubiquitous and others more tissue-specific .

Biological Functions

Deaminases play essential roles in various biological processes. ADA, for example, is vital for the development and maintenance of the immune system . ACC deaminase helps plants cope with biotic and abiotic stresses by reducing ethylene levels, which can inhibit plant growth . Deaminases are also involved in pathogen recognition and immune responses, contributing to the body’s defense mechanisms .

Modes of Action

The modes of action of deaminases involve interactions with other molecules and cells. ACC deaminase, for instance, breaks down ACC into ammonia and α-ketobutyrate, which are further metabolized by bacteria . ADA catalyzes the deamination of adenosine to inosine, which is crucial for nucleotide metabolism . These enzymes often have specific binding partners and can initiate downstream signaling cascades that regulate various cellular processes .

Regulatory Mechanisms

The expression and activity of deaminases are tightly regulated. ACC deaminase synthesis is induced by its substrate ACC and regulated by environmental conditions . Transcriptional regulation involves regulatory genes such as LRP protein and other elements activated under different conditions . Post-translational modifications also play a role in modulating the activity of deaminases, ensuring they function appropriately in response to cellular needs .

Applications

Deaminases have significant applications in biomedical research, diagnostics, and therapeutics. ADA deficiency, for example, leads to severe combined immunodeficiency (SCID), and enzyme replacement therapy is a treatment option . ACC deaminase is used in agriculture to enhance plant growth under stress conditions . Cytidine deaminases are employed in gene editing technologies, such as CRISPR, to introduce specific mutations .

Role in the Life Cycle

Deaminases play crucial roles throughout the life cycle, from development to aging and disease. ADA is essential for immune system development and function . ACC deaminase helps plants grow and adapt to environmental stresses, promoting survival and productivity . The activity of deaminases can also influence aging processes and the progression of diseases, highlighting their importance in maintaining cellular homeostasis .

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