MAPK1 Human

Mitogen-Activated Protein Kinase 1 Human Recombinant

MAPK1 Recombinant, also known as extracellular signal-regulated kinase, is a Mitogen-Activated Protein Kinase. This highly active form is produced through in vitro phosphorylation of purified ERK2/MAPK1 by MEK1. It is a non-glycosylated polypeptide with a molecular weight of 44.6 kDa. The purification of MAPK1 is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11406
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

MAPK1 Human, Active

Mitogen-Activated Protein Kinase 1 Human Recombinant, Active

Recombinant human MAPK1 is a full-length protein with a molecular weight of 41,762 Daltons. It is produced without glycosylation and encompasses amino acids Met1 to Ser360. The protein is purified using advanced chromatographic methods, ensuring high purity.
Shipped with Ice Packs
Cat. No.
BT11487
Source
Escherichia Coli.
Appearance
Clear solution, sterile-filtered.

MAPK1 Human, His

Recombinant Human Mitogen-Activated Protein Kinase 1, His Tag

Recombinant human MAPK1, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-360 a.a.) with a molecular weight of 43.5 kDa. A 20 amino acid His-tag is fused to the N-terminus of MAPK1, which is then purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11571
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterile filtered.

MAPK11 Human

Mitogen-Activated Protein Kinase 11 Human Recombinant

Recombinant human MAPK11, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 387 amino acids (residues 1-364) with a molecular weight of 43.8 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11649
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.

MAPK3 Human, His

Mitogen-Activated Protein Kinase 3 Human Recombinant, His-Tag

Recombinant Human MAPK3, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 399 amino acids (residues 1-379) with a molecular weight of 45.2 kDa. The MAPK3 protein is fused to a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12056
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

MAP2K1 Human

Mitogen-Activated Protein Kinase Kinase 1 Human Recombinant

Produced in Sf9 Baculovirus cells, MAP2K1 is a single, glycosylated polypeptide chain composed of 402 amino acids (1-393a.a.) with a molecular mass of 44.5 kDa. Note that on SDS-PAGE, its molecular size appears between 40-57 kDa. This MAP2K1 protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT11004
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless solution, sterile-filtered.

MAP2K2 Human

Mitogen-Activated Protein Kinase Kinase 2 Human Recombinant

Recombinant human MAP2K2, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 424 amino acids (with residues 1-400 representing the MAP2K2 sequence) and possessing a molecular weight of 46.9 kDa. This protein features a 24 amino acid His-tag fused at its N-terminus and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11087
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

MAP2K3 Human

Mitogen-Activated Protein Kinase Kinase 3 Human Recombinant

Recombinant human MAP2K3, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 338 amino acids (residues 1-318) with a molecular weight of 38.3 kDa. A 20 amino acid His-tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11174
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

MAPK10 Human

Mitogen-Activated Protein Kinase 10 Human Recombinant

MAPK10, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain with a molecular weight of 53.4 kDa. It consists of 470 amino acids (1-464 a.a.). The protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10906
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution, sterile-filtered.

MAPK8 Human

Mitogen-Activated Protein Kinase 8 Human Recombinant

Recombinant Human MAPK8, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 450 amino acids (1-427a.a) with a molecular weight of 50.5 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT12146
Source
E.coli.
Appearance
A clear and colorless solution that has been sterilized by filtration.
Definition and Classification

Mitogen-Activated Protein Kinases (MAPKs) are a family of serine/threonine-specific protein kinases involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock, and proinflammatory cytokines . They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis . MAPKs are found exclusively in eukaryotes and are classified into three main subfamilies:

  • Extracellular signal-regulated kinases (ERKs)
  • c-Jun N-terminal kinases (JNKs)
  • p38 mitogen-activated protein kinases (p38s) .
Biological Properties

Key Biological Properties: MAPKs are involved in various cellular processes, including proliferation, differentiation, motility, and survival . They are activated by dual phosphorylation events and have a three-tiered pathway architecture .

Expression Patterns and Tissue Distribution: MAPKs are ubiquitously expressed in all eukaryotic cells, including animals, fungi, plants, and unicellular eukaryotes . They are particularly abundant in tissues that are frequently exposed to stress, such as the skin, liver, and immune cells .

Biological Functions

Primary Biological Functions: MAPKs play crucial roles in regulating cell proliferation, differentiation, motility, and survival . They are also involved in immune responses and pathogen recognition by activating various transcriptional programs that lead to proinflammatory responses .

Role in Immune Responses and Pathogen Recognition: MAPKs are essential for the activation of innate immune responses against invading microbial pathogens . They translocate into the nucleus and activate transcription factors that alter gene expression, leading to the production of proinflammatory cytokines .

Modes of Action

Mechanisms with Other Molecules and Cells: MAPKs interact with various molecules and cells through phosphorylation cascades. They are activated by upstream kinases (MAPKKKs and MAPKKs) and, in turn, phosphorylate downstream substrates .

Binding Partners and Downstream Signaling Cascades: MAPKs bind to scaffolding proteins that organize the signaling complexes and ensure specificity in signal transduction . They activate a wide range of downstream signaling cascades that regulate gene expression, cell cycle progression, and apoptosis .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The activity of MAPKs is tightly regulated by phosphorylation of their activation loop, which can be modulated by positive and negative feedback mechanisms .

Transcriptional Regulation and Post-Translational Modifications: MAPKs are regulated at the transcriptional level by various transcription factors. Post-translational modifications, such as phosphorylation, ubiquitination, and sumoylation, also play crucial roles in modulating their activity .

Applications

Biomedical Research: MAPKs are extensively studied in biomedical research for their roles in cell signaling and disease mechanisms .

Diagnostic Tools: MAPKs serve as biomarkers for various diseases, including cancer and inflammatory disorders .

Therapeutic Strategies: Targeting MAPK pathways has therapeutic potential in treating diseases such as cancer, autoimmune disorders, and neurodegenerative diseases .

Role in the Life Cycle

Role Throughout the Life Cycle: MAPKs are involved in various stages of the life cycle, from development to aging and disease . They regulate embryogenesis, cell differentiation, and tissue homeostasis . Dysregulation of MAPK pathways is associated with aging and age-related diseases, such as cancer and neurodegeneration .

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