AHCY Mouse

Adenosylhomocysteinase Mouse Recombinant

Recombinant AHCY from mouse has been expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. This protein consists of 456 amino acids, with a molecular weight of 50.2 kDa, including a 24 amino acid His-tag fused at the N-terminus (amino acids 1-432). Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26078
Source
Escherichia Coli.
Appearance
The product is a clear and colorless solution that has been sterilized through filtration.

AHCY Human

Adenosylhomocysteinase Human Recombinant

This product consists of the recombinant human AHCY enzyme, produced in E. coli. It is a single, non-glycosylated polypeptide chain with 452 amino acids, corresponding to amino acids 1-432 of the native protein. The molecular weight of the recombinant AHCY is 49.8 kDa. For purification and detection purposes, a 20 amino acid His-Tag is fused to the N-terminus of the protein. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25929
Source
Escherichia Coli.
Appearance
The product is a clear, colorless solution that has been sterilized by filtration.

AHCY Human, Sf9

Adenosylhomocysteinase Human Recombinant, Sf9

Recombinant human AHCY, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 441 amino acids (1-432 a.a.). It has a molecular weight of 48.8kDa and appears as a band between 40-57kDa on SDS-PAGE under reducing conditions. The AHCY protein is fused with a 6 amino acid His-Tag at the C-terminus and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT25999
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution, sterile-filtered.
Definition and Classification

Adenosylhomocysteinase (AHCY), also known as S-adenosyl-L-homocysteine hydrolase (SAHH), is a protein-coding gene. It belongs to the adenosylhomocysteinase family and catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine and L-homocysteine . This enzyme plays a crucial role in regulating intracellular SAH concentration, which is important for transmethylation reactions .

Biological Properties

AHCY is one of the most conserved enzymes across various species, including bacteria, nematodes, yeast, plants, insects, and vertebrates . It is the only enzyme in mammals capable of catalyzing the reversible hydrolysis of SAH . AHCY is recruited to chromatin during replication and active transcription, correlating with increasing demands for DNA, RNA, and histone methylation . The enzyme is expressed in various tissues, with significant roles in metabolic processes .

Biological Functions

AHCY acts within the one-carbon metabolic cycle, a universal metabolic process that enables the transfer of one-carbon units for biosynthetic processes, amino acid homeostasis, redox cellular control, and epigenetic regulation . It is essential for the breakdown of methionine and the regulation of methylation levels . AHCY deficiency is associated with hypermethioninemia, developmental delays, and potentially fatal outcomes .

Modes of Action

AHCY catalyzes the hydrolysis of SAH to adenosine and L-homocysteine, thus regulating the methylation potential within cells . It interacts with various molecules and is involved in the regulation of DNA methylation throughout the day by interacting with circadian rhythm genes . AHCY’s activity is crucial for maintaining the balance of SAM (S-adenosylmethionine) and SAH, which is vital for numerous cellular processes .

Regulatory Mechanisms

The expression and activity of AHCY are regulated at multiple levels. Transcriptional regulation involves various factors that control the gene’s expression . Post-translational modifications, such as acetylation, can significantly impact AHCY’s catalytic activity . Additionally, AHCY’s subcellular localization is controlled to facilitate local transmethylation reactions .

Applications

AHCY has several applications in biomedical research. Antibodies targeting AHCY are used in various scientific applications, including Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation . AHCY’s role in regulating methylation makes it a potential target for therapeutic strategies aimed at diseases related to methylation imbalances .

Role in the Life Cycle

AHCY plays a critical role throughout the life cycle. It is essential for embryonic development, as its deletion is embryonic lethal in many organisms . AHCY’s regulation of methylation and metabolic processes is crucial for maintaining cellular homeostasis from development to aging . Dysregulation of AHCY activity is associated with various diseases, including cancer .

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