DEFB116 Human

Beta Defensin 116 Human Recombinant

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Cat. No.

BT15551

Source

Escherichia Coli.

Synonyms

Beta-Defensin 16, DEFB-16, Beta 16, defensin, Beta-Defensin 116, Defensin, Beta 16, DEFB16.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Introduction to DEFB116 Human

DEFB116 Human is a recombinant human beta-defensin peptide, a key component of the innate immune system. Belonging to the beta-defensin family, it is a small cationic peptide (11.5 kDa) characterized by its broad-spectrum antimicrobial activity against bacteria, fungi, and viruses. Produced via bacterial expression systems (e.g., Escherichia coli), it is non-glycosylated and contains 102 amino acids (residues 24–102) with a His-tag fusion for purification .

Table 1: Key Molecular Properties of DEFB116 Human

Property	Value/Description	Source
Molecular Mass	11.5 kDa
Amino Acid Sequence	M G S S H H H H H H S S G L V P R G S H M G S G L F R S H N G K S R E P W N P C E L Y Q G M C R N A C R E Y E I Q Y L T C P N D Q K C C L K L S V K I T S S K N V K E D Y D S N S N L S V T N S S S Y S H I
Expression System	E. coli
Purity	>80% (SDS-PAGE confirmed)
Stability	Store at -20°C; avoid freeze-thaw cycles

Biological Functions and Mechanisms

DEFB116 Human exhibits antimicrobial activity through membrane disruption and interference with microbial metabolic processes . Key functions include:

Table 2: Potential Therapeutic Targets for DEFB116 Human

Disease/Infection	Proposed Mechanism	Clinical Relevance
Bacterial Skin Infections	Membrane disruption of pathogens	Alternative to antibiotics
Respiratory Tract Infections	Neutralization of viral/fungal agents	Adjunct therapy for antiviral drugs
Multidrug-Resistant Pathogens	Novel target for antibiotic resistance	Preclinical studies underway

DEFB116 Human is under investigation as a therapeutic agent due to its:

High Efficacy: Demonstrated in vitro antibacterial activity .
Low Immunogenicity: Recombinant production minimizes immune cross-reactivity .
Drug Delivery Potential: Formulation in medical devices or topical treatments for long-term antibacterial activity .

Production and Characterization

Expression Systems: Recombinant DEFB116 is typically purified via nickel affinity chromatography due to its His-tag fusion .
Quality Control: SDS-PAGE and Western blotting confirm purity and structural integrity .

Challenges in Clinical Translation

Stability Issues: Requires stabilization with carrier proteins (e.g., HSA, BSA) for long-term storage .
Toxicity Concerns: High concentrations may induce host cell apoptosis, necessitating dose optimization .

Product Specs

Introduction

Beta Defensin 116, also known as DEFB116, is a member of the beta-defensin family that exhibits antibacterial activity. DEFB116 belongs to the innate immune system, which utilizes antimicrobial peptides to protect multicellular organisms from a wide range of microorganisms. Beta-Defensins are a crucial family of mammalian antimicrobial peptides.

Description

Recombinant human DEFB116, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 102 amino acids (24-102 a.a) with a molecular weight of 11.5 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.

Physical Appearance

A clear solution that has undergone sterile filtration.

Formulation

DEFB116 protein solution at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.

Stability

For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.

Purity

The purity of the DEFB116 protein is greater than 80.0%, as determined by SDS-PAGE analysis.

Synonyms

Beta-Defensin 16, DEFB-16, Beta 16, defensin, Beta-Defensin 116, Defensin, Beta 16, DEFB16.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSGLFRSHN GKSREPWNPC ELYQGMCRNA CREYEIQYLT CPNDQKCCLK LSVKITSSKN VKEDYDSNSN LSVTNSSSYS HI.

Product Science Overview

Structure and Function

Beta-defensins are small, cationic peptides characterized by their beta-sheet structure stabilized by disulfide bonds. DEFB116, like other beta-defensins, exhibits antimicrobial activity, which is vital for the host defense mechanism. The gene encoding DEFB116 is located on chromosome 20 and is densely clustered with other beta-defensin genes.

Expression and Regulation

The expression of DEFB116 is regulated by various factors, including microbial components and inflammatory cytokines. This regulation ensures that DEFB116 is produced in response to infection or inflammation, thereby contributing to the body’s defense against pathogens.

Recombinant Production

Recombinant human DEFB116 is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is often fused with a His-tag at the N-terminus to facilitate purification. The recombinant DEFB116 protein is typically used in research to study its antimicrobial properties and potential therapeutic applications.

Applications in Research

Recombinant DEFB116 is valuable in various research applications, including:

Antimicrobial assays: To evaluate its effectiveness against different microbial strains.
Immunological studies: To understand its role in the immune response.
Drug development: As a potential candidate for developing new antimicrobial agents.

Storage and Handling

Recombinant DEFB116 should be stored at 4°C for short-term use and at -20°C for long-term storage. It is essential to avoid repeated freeze-thaw cycles to maintain its stability and activity.

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DEFB116 Human

Description

Introduction to DEFB116 Human

Table 1: Key Molecular Properties of DEFB116 Human

Biological Functions and Mechanisms

Table 2: Potential Therapeutic Targets for DEFB116 Human

Production and Characterization

Challenges in Clinical Translation

Product Specs

Product Science Overview

Quick Inquiry

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