FAM107B Human
Description
Gene Structure and Evolutionary Conservation
FAM107B (Family With Sequence Similarity 107 Member B) is a human gene located on chromosome 10p13, encoding a 18 kDa nuclear protein . Key structural features include:
FAM107B belongs to the FAM107 family, which includes FAM107A (TU3A/DRR1). Both share conserved DUF1151 domains but differ in C-terminal coiled-coil regions .
Protein Characteristics and Functional Domains
FAM107B is an 18 kDa protein with distinct structural and functional properties:
Comparison with FAM107A
Functional Roles in Cellular Biology
FAM107B exhibits multifaceted roles in cellular homeostasis and cancer biology:
Tumor Suppression
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Downregulation in Cancers: Reduced expression in gastric, colorectal, lung, and breast cancers .
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Growth Inhibition: Forced expression suppresses proliferation and migration in gastric cancer cells (e.g., MGC803) .
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Heat Shock Induction: Promoter contains heat shock transcription factor (HSF) binding sites, enabling stress-responsive upregulation .
Transcriptional Regulation
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HAT Complex Modulation: Interacts with Tada3α, a core component of histone acetyltransferase (HAT) complexes .
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Oncogene Suppression: Inhibits S100A4-mediated pathways, reducing tumor invasion .
Stress Response
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Heat Shock Protein (HSP) Analog: Shares functional similarity with HSPs but lacks oncogenic properties .
Clinical Implications in Cancer
FAM107B’s dysregulation is linked to diverse malignancies:
Mechanistic Insights
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S100A4 Interaction: FAM107B mediates S100A4’s effects on gastric cancer cell migration .
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Epigenetic Silencing: Promoter methylation observed in aflatoxin B1-exposed cells .
Research Findings and Experimental Models
Key studies highlight FAM107B’s regulatory networks:
Future Directions and Unresolved Questions
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Mechanistic Elucidation:
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Role of DUF1151 in transcriptional regulation.
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Therapeutic potential in restoring FAM107B expression in cancers.
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Diagnostic Utility:
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Validation as a biomarker for gastrointestinal cancers.
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Stress Response Dynamics:
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Synergy between heat shock induction and chemotherapeutic efficacy.
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Product Specs
Product Science Overview
FAM107B is located on chromosome 10 and is also known by other names such as C10orf45, FLJ45505, and HITS . The gene encodes a protein that consists of 154 amino acids and has a molecular mass of approximately 17.9 kDa . The recombinant form of FAM107B is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain . It is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .
FAM107B is predicted to play a role in the sensory perception of sound . It is involved in various cellular processes, including cell proliferation, immune response, and protein ubiquitination . The protein is expressed in multiple tissues, including lymphoid tissue, bone marrow, testis, skeletal muscle, and various immune cells .
Mutations or dysregulation of FAM107B have been linked to several diseases. Notably, it is associated with tibial neuropathy and cataract 13 with adult I phenotype . These associations highlight the potential clinical significance of FAM107B and its importance in maintaining normal physiological functions.
The recombinant form of FAM107B is valuable in research settings. It allows scientists to study the protein’s function, interactions, and role in disease mechanisms. The availability of recombinant FAM107B facilitates various experimental approaches, including structural analysis, functional assays, and therapeutic research.
