FAM3C Human

Family with Sequence Similarity 3, Member C Human Recombinant
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Cat. No.
BT7931
Source
E.coli.
Synonyms
Family with sequence similarity 3 member C, ILEI, Interleukin-like EMT inducer, predicted osteoblast protein, GS3876, protein FAM3C.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Introduction to FAM3C Human

FAM3C, also termed Interleukin-like EMT inducer (ILEI), belongs to the FAM3 family of cytokine-like proteins. This 224-amino acid protein features a conserved GG domain and is expressed across multiple tissues, including the brain, pancreas, and epithelial cells . Its secretion and interaction with extracellular components enable roles in cell signaling, differentiation, and disease pathogenesis .

Cancer

Elevated FAM3C expression is strongly linked to aggressive tumor behavior and poor prognosis across cancers:

Cancer TypeRole of FAM3CPrognostic Impact
Pancreatic (PAAD)Promotes proliferation and invasionHigh = Worse survival
BreastActivates YY1-HSF1 signaling axisMetastasis driver
GastricEnhances PI3K-Akt pathway activationPoor differentiation
ColorectalSuppresses NF-κB signalingReduced patient survival

Mechanistically, FAM3C drives epithelial-mesenchymal transition (EMT) and upregulates oncogenic pathways like p53 signaling .

Alzheimer’s Disease

Low FAM3C levels in the brain are associated with AD pathogenesis, potentially serving as a biomarker alongside saposin D .

Mechanistic Insights and Signaling Pathways

FAM3C regulates multiple pathways:

  • PI3K-Akt: Promotes cell migration in gastric cancer .

  • p53 Signaling: Modulates apoptosis and DNA repair in PAAD .

  • YY1-HSF1 Axis: Drives proliferation in breast cancer .

In C. elegans, FAM3C ortholog FAMP-1 governs memory-based thermotaxis, highlighting conserved roles in neural circuits .

Human FAM3C ELISA Kit

ParameterSpecification
Detection Range15.625–1000 pg/mL
Sensitivity<9.375 pg/mL
Sample TypesSerum, plasma, cell culture supernatants
ApplicationsQuantifying FAM3C in metabolic studies, cancer research

Recombinant FAM3C protein (HEK293-derived) is utilized for in vitro assays, enabling studies on retinal lamination and metabolic disorders .

Therapeutic Potential

FAM3C is emerging as a dual-purpose target:

  • Biomarker: Serum levels correlate with AD progression and cancer aggressiveness .

  • Therapeutic Target: Inhibitors under investigation for disrupting FAM3C-driven pathways in PAAD and breast cancer .

Future Directions

  • Clinical Trials: Validating FAM3C’s utility in non-invasive diagnostics.

  • Pathway Modulation: Developing small molecules to block FAM3C’s interaction with PI3K-Akt and p53.

Product Specs

Introduction
FAM3C belongs to the FAM3 family and possesses a GG domain. The expression of this protein is differentially regulated in pancreatic cancer-derived cells. Serving as a downstream target gene for the Nkx5-1 transcription factor, FAM3C participates in cell differentiation and proliferation during inner ear embryogenesis. Additionally, FAM3C plays a role in retinal laminar formation and promotes the transition from epithelial to mesenchymal cells.
Description
Recombinant human FAM3C, produced in E.coli, is a single, non-glycosylated polypeptide chain. It comprises 224 amino acids (residues 25-227) and has a molecular weight of 24.5 kDa. The protein is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that is sterile filtered.
Formulation
The FAM3C solution is provided at a concentration of 0.5mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 200mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store the solution at 4°C. For longer storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
The purity of FAM3C is determined by SDS-PAGE analysis to be greater than 90%.
Synonyms
Family with sequence similarity 3 member C, ILEI, Interleukin-like EMT inducer, predicted osteoblast protein, GS3876, protein FAM3C.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MQVFEIKMDA SLGNLFARSA LDTAARSTKP PRYKCGISKA CPEKHFAFKM ASGAANVVGP KICLEDNVLM SGVKNNVGRG INVALANGKT GEVLDTKYFD MWGGDVAPFI EFLKAIQDGT IVLMGTYDDG ATKLNDEARR LIADLGSTSI TNLGFRDNWV FCGGKGIKTK SPFEQHIKNN KDTNKYEGWP EVVEMEGCIP QKQD

Product Science Overview

Gene and Protein Structure

The FAM3C gene is located on chromosome 7 and encodes a protein consisting of 227 amino acids . The protein includes an N-terminal signal peptide, which is crucial for its secretion . The GG domain within the protein is a defining feature of the FAM3 family .

Function and Biological Role

FAM3C plays a significant role in various biological processes:

  • Epithelial-Mesenchymal Transition (EMT): FAM3C is known to promote EMT, a process where epithelial cells lose their cell polarity and adhesion properties to become mesenchymal stem cells. This transition is essential for various developmental processes and has implications in cancer metastasis .
  • Retinal Laminar Formation: FAM3C may be involved in the formation of retinal layers, indicating its role in eye development .
  • Cytokine Activity: The protein exhibits cytokine activity, which is involved in cell signaling and immune responses .
Clinical Significance

Changes in the expression of FAM3C have been observed in pancreatic cancer-derived cells, suggesting its potential role in cancer biology . Additionally, FAM3C has been associated with diseases such as Autism Spectrum Disorder and Dopa-Responsive Dystonia .

Recombinant FAM3C

Recombinant FAM3C proteins are produced using various expression systems, such as E. coli, for research purposes. These recombinant proteins are used in studies to understand the protein’s function, interactions, and potential therapeutic applications .

Research and Applications

FAM3C is ubiquitously expressed in most tissues, making it a valuable target for research in developmental biology, cancer research, and immunology . Studies involving recombinant FAM3C help in elucidating its role in EMT and its potential as a therapeutic target in cancer treatment .

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