FGF19 Human, HEK
Description
Overview of FGF19 Human, HEK
Recombinant human FGF-19 produced in HEK293 cells is a critical tool for studying metabolic regulation, bile acid synthesis, and hepatocellular signaling. This protein belongs to the endocrine FGF subfamily and primarily acts via FGFR4 in the liver, with additional interactions mediated by β-Klotho or α-Klotho cofactors .
Primary Structure
FGF19 Human, HEK contains 229 amino acids (mature form), with a C-terminal domain critical for receptor specificity. Key features:
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His-tag: C-terminal polyhistidine tag for purification and detection .
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C-terminal tail: Determines binding to β-Klotho (for metabolic effects) or α-Klotho (uncommon) .
Receptor Interactions
FGF19 exhibits unique binding profiles:
Metabolic Regulation
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Bile acid synthesis: Suppresses CYP7A1/CYP8B1 via FXR-SHP pathway .
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Glucose/lipid metabolism: Enhances insulin sensitivity, reduces hepatic triglycerides .
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Hepatocyte proliferation: Induces BrdU incorporation and AFP expression, linked to hepatocellular carcinoma (HCC) .
Atherosclerosis and Cardiovascular Disease
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Predictive biomarker: Elevated serum FGF19 levels correlate with subclinical atherosclerosis (IMT) in men with T2D (OR = 4.798 per SD) .
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Mechanism: May reflect compensatory responses to dyslipidemia or bile acid-mediated vascular damage .
Experimental Models
Therapeutic Potential
Product Specs
Fibroblast Growth Factor-19 (FGF-19) is a member of the FGF family that participates in various physiological processes, including bile acid biosynthesis regulation and glucose metabolism. FGF-19 binds to fibroblast growth factor receptors (FGFRs), particularly FGFR4, and initiates downstream signaling cascades. This growth factor plays a crucial role in maintaining metabolic homeostasis and has been implicated in various diseases.
Recombinant Human FGF19 is produced in HEK293 cells. This protein consists of a single glycosylated polypeptide chain with 205 amino acids (a.a 23-216), resulting in a molecular weight of 23.0 kDa. For purification purposes, a 6 amino acid His-tag is fused to the C-terminus, and proprietary chromatographic techniques are employed.
The FGF19 protein solution has a concentration of 0.5mg/ml and is supplied in a buffer consisting of 20mM Tris-HCl (pH 8.0), 20% glycerol, and 0.1M NaCl.
Repeated freezing and thawing should be avoided.
The purity of this product is greater than 95.0%, as determined by SDS-PAGE analysis.
fibroblast growth factor 19, FGF19.
HEK293 cells.
DGSHMRPLAF SDAGPHVHYG WGDPIRLRHL YTSGPHGLSS CFLRIRADGV VDCARGQSAH SLLEIKAVAL RTVAIKGVHS VRYLCMGADG KMQGLLQYSE EDCAFEEEIR PDGYNVYRSE KHRLPVSLSS AKQRQLYKNR GFLPLSHFLP MLPMVPEEPE DLRGHLESDM FSSPLETDSM DPFGLVTGLE AVRSPSFEKH HHHHH
Product Science Overview
FGF19 is a protein encoded by the FGF19 gene, located on chromosome 11q13.3 in humans . The protein consists of 216 amino acids and contains a signal sequence and two conserved cysteine residues . It is expressed in fetal brain tissue but not in adult brain tissue . The human recombinant version of FGF19 is often produced in HEK 293 cells, a human embryonic kidney cell line, to ensure proper folding and post-translational modifications .
FGF19 plays a crucial role in regulating carbohydrate, lipid, and bile acid metabolism . It acts as a hormone-like protein that can stimulate hepatic protein and glycogen synthesis without inducing lipogenesis . The effects of FGF19 are mediated through the mitogen-activated protein kinase (MAPK) signaling pathway, which activates components of the protein translation machinery and stimulates glycogen synthase activity .
Recombinant human FGF19 is used in various research applications, including:
The lyophilized form of recombinant human FGF19 should be stored at room temperature and reconstituted with phosphate-buffered saline (PBS) before use . Once reconstituted, it can be aliquoted and stored at -80°C for long-term storage or at +4°C for short-term use . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .
As an active protein, FGF19 can elicit biological responses in vivo, so it should be handled with caution . Researchers should follow appropriate safety guidelines and protocols when working with this protein.
In summary, Fibroblast Growth Factor-19 (Human Recombinant, HEK) is a valuable tool in scientific research, offering insights into various biological processes and potential therapeutic applications. Its high purity and biological activity make it suitable for a wide range of experimental studies.
