GRO b Mouse

GRO-Beta Mouse Recombinant (CXCL2)
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Cat. No.
BT13699
Source
Escherichia Coli.
Synonyms
Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine (C-X-C motif) ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GRO-Beta Mouse Recombinant also caled mouse MIP-2 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7849 Dalton.
The CXCL2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta), and Gro oncogene-2 (Gro-2), is a small cytokine of the CXC chemokine family. CXCL2 shares 90% amino acid sequence identity with CXCL1, a related chemokine. Produced by monocytes and macrophages, CXCL2 exhibits chemotactic properties for polymorphonuclear leukocytes and hematopoietic stem cells. The CXCL2 gene is found on human chromosome 4 within a cluster of other CXC chemokine genes. CXCL2 exerts its cell-mobilizing effects by interacting with CXCR2, a cell surface chemokine receptor.
Description
GRO-Beta Mouse Recombinant, also known as mouse MIP-2, is produced in E. coli. It is a single, non-glycosylated polypeptide chain composed of 73 amino acids with a molecular weight of 7849 Daltons. The purification of CXCL2 is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated solution (1.0mg/ml) in 20mM PB, pH 7.4, 150mM NaCl.
Solubility
To reconstitute the lyophilized GRO-beta Mouse, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized CXCL2 is stable at room temperature for 3 weeks, but it is recommended to store it desiccated below -18°C. After reconstitution, CXCL2 should be stored at 4°C for 2-7 days. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% using the following methods:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity
The biological activity is assessed based on the ability to chemoattract total human neutrophils. This is measured within a concentration range of 1.0-10.0 ng/ml, corresponding to a specific activity of 10^5-10^6 units/mg.
Protein Content
The protein content is quantified using two independent methods:
1. UV spectroscopy at 280 nm. An absorbency value of 0.015 is used as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GRO-b as a Reference Standard.
Synonyms
Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine (C-X-C motif) ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta.
Source
Escherichia Coli.
Amino Acid Sequence
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN.

Product Science Overview

Discovery and Structure

GRO-Beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation . It is a member of the alpha (C-X-C) subfamily of chemokines, which are characterized by the presence of a conserved cysteine motif separated by a single amino acid .

Function and Mechanism

The primary function of GRO-Beta is to act as a chemoattractant for neutrophils, guiding them to sites of inflammation or infection. It achieves this by binding to the CXCR2 receptor on the surface of neutrophils, which triggers a signaling cascade that results in the migration of these immune cells to the affected area . This process is essential for the body’s defense against infections and for the resolution of inflammation.

Recombinant Production

Recombinant GRO-Beta (Mouse) is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>95%) and is available in both carrier-free and carrier-containing formulations . The carrier protein, often Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .

Applications

Recombinant GRO-Beta is widely used in research to study its role in immune responses and inflammation. It is also used in various assays to investigate the signaling pathways involved in chemokine-mediated cell migration and activation .

Storage and Stability

Recombinant GRO-Beta is typically lyophilized and can be stored at -20°C to -80°C for up to twelve months. Once reconstituted, it should be stored under sterile conditions and aliquoted to avoid repeated freeze-thaw cycles .

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