HBsAg Paired Antibody
Description
Definition and Purpose
HBsAg paired antibodies consist of two monoclonal antibodies that bind to distinct, non-overlapping epitopes on HBsAg. This configuration enables:
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Sandwich immunoassays: One antibody captures HBsAg, while the other detects it ( ).
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Epitope diversity: Targeting multiple regions reduces the risk of diagnostic escape due to HBsAg mutations ( ).
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Enhanced sensitivity: Paired systems detect HBsAg at lower concentrations compared to single-antibody methods ( ).
Key Features
Example Pair
Lateral Flow Immunoassays
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Mechanism: Paired antibodies enable rapid, point-of-care detection of HBsAg in serum ( ).
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Advantages: Avoids false negatives caused by epitope mutations (e.g., G145R/A) ( ).
ELISA Platforms
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Sensitivity: Detects HBsAg at concentrations as low as 0.05–0.1 IU/mL ( ).
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Clinical Use: Critical for early HBV diagnosis, vaccine efficacy monitoring, and HBsAg seroclearance assessment ( ).
Neutralization Strategies
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Combination Therapy: Broadly neutralizing antibodies (bNAbs) targeting non-overlapping HBsAg epitopes suppress viral rebound in humanized mice ( ).
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Resistance Prevention: Antibody pairs (e.g., Group I + III bNAbs) reduce escape mutations by 89% compared to single antibodies ( ).
Key Findings
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Epitope Conservation: Structural analysis reveals that paired antibodies stabilize critical HBsAg loops (e.g., residues 139–147), hindering immune evasion ( ).
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Synergistic Effects: Combinations like H017 + H019 achieve 99% viral load reduction in chronic HBV models ( ).
Paired vs. Single Antibodies
Challenges and Innovations
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Mutation Surveillance: Emerging HBsAg variants (e.g., pre-S1 deletions) necessitate ongoing antibody pair optimization ( ).
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Recombinant Production: Advances in E. coli-expressed antibodies (e.g., His-tagged constructs) improve scalability and cost-efficiency ( ).
Future Directions
Product Specs
Purified by protein A chromatography.
Product Science Overview
Hepatitis B virus (HBV) is a small, partially double-stranded DNA virus that belongs to the genus Orthohepadnaviruses of the Hepadnaviridae family. It is a bloodborne virus transmitted through contact with infected blood or bodily fluids. HBV infection can lead to liver diseases ranging from acute or chronic hepatitis to cirrhosis and hepatocellular carcinoma .
The surface antigen of the Hepatitis B virus, known as HBsAg, is a protein that forms part of the virus’s outer envelope. It was initially referred to as the Australia antigen when first identified in the 1960s . HBsAg is crucial for the virus’s ability to infect liver cells and is a key marker used in diagnosing HBV infection.
Mouse anti-Hepatitis B surface antigen antibodies are monoclonal antibodies developed to specifically target and bind to HBsAg. These antibodies are produced by immunizing mice with HBsAg and then harvesting the antibody-producing cells. The cells are fused with myeloma cells to create hybridomas, which can be cultured to produce large quantities of the monoclonal antibody .
Paired antibodies refer to two monoclonal antibodies that are used together in immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay), to detect the presence of HBsAg. One antibody, known as the capture antibody, is coated onto a microtiter plate and binds to HBsAg present in the sample. The second antibody, known as the detection antibody, is typically labeled with an enzyme or fluorescent marker and binds to a different epitope on the HBsAg, allowing for the detection and quantification of the antigen .
Mouse anti-HBsAg paired antibodies are widely used in diagnostic assays to detect HBV infection. These assays are crucial for screening blood donations, diagnosing acute and chronic HBV infections, and monitoring the effectiveness of HBV vaccination programs . Additionally, these antibodies are used in research to study the structure and function of HBsAg and to develop new therapeutic strategies for HBV infection.
