IL 3 Mouse
Description
Hematopoiesis and Immune Modulation
IL-3 Mouse stimulates:
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Hematopoietic stem/progenitor cells: Supports colony formation of erythroid, megakaryocyte, neutrophil, and monocytic lineages .
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Mature immune cells: Enhances survival and activity of mast cells, eosinophils, basophils, and macrophages .
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Synergistic effects: Amplifies responses when combined with GM-CSF or IL-5 .
Signaling Mechanisms
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Receptor binding: IL-3 binds a heterodimeric receptor (α/β subunits) with high affinity (Kd: 10⁻⁹–10⁻¹⁰ M) .
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Downstream pathways: Activates JAK/STAT, Ras-Raf-ERK, and PI3K/Akt, promoting anti-apoptotic signals via Bcl-2 .
Disease Models
In Vitro Applications
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Cell culture: Used at 0.1–10 ng/mL for maintaining IL-3-dependent cell lines (e.g., FDC-P1, M-NFS-60) .
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Signal transduction studies: Identified phosphorylation of 150 kDa glycoprotein upon receptor binding .
Species Specificity and Limitations
Product Specs
Product Science Overview
IL-3 exerts its biological effects through interaction with a specific cell surface receptor complex. The mouse IL-3 receptor consists of a unique alpha-subunit (CD123) and two beta subunits: one specific for IL-3 (βIL-3) and the other shared with the receptors for IL-5 and GM-CSF (beta common chain, βc or CD131) . Binding of IL-3 to its receptor activates several intracellular signaling pathways, including JAK/STAT, MAPK, and PI3K .
IL-3 is involved in various physiological processes, including:
- Hematopoiesis: IL-3 promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells .
- Inflammation and Allergies: IL-3 plays a role in inflammation and allergic responses by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by mast cells, basophils, and eosinophils .
- Cell Differentiation: IL-3 supports the differentiation of hematopoietic progenitor cells into various mature blood cell lineages .
