IL3 Mouse, sf9
Description
Functional Insights
IL3 Mouse, sf9 binds to high-affinity receptors composed of α and β subunits (AIC2A/AIC2B in mice), triggering signaling pathways that regulate:
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Hematopoiesis: Supports growth of myeloid precursors, mast cells, eosinophils, and basophils .
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Neurotrophic Activity: Modulates microglial function in neurodegenerative diseases like Alzheimer’s .
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Immune Regulation: Amplifies inflammatory responses in autoimmune diseases (e.g., lupus, multiple sclerosis) .
Studies in A/J mice revealed that defects in IL-3 receptor α-subunit splicing impair hematopoietic colony formation, underscoring IL-3’s non-redundant role in hematopoiesis .
In Vitro Models
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Cell Proliferation Assays: ED50 < 0.1 ng/mL in TF-1 cell lines, indicating high specific activity (10,000,000 IU/mg) .
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Receptor Binding Studies: Structural analyses of IL-3Rα/β complexes reveal two distinct binding modes critical for signaling .
Disease Mechanisms
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Neurodegeneration: IL-3 reprograms microglia to clear amyloid-β aggregates in Alzheimer’s models but exacerbates neuroinflammation in multiple sclerosis .
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Autoimmunity: Elevated IL-3 correlates with lupus nephritis severity and IFNα production in SLE patients .
Comparative Analysis: Mouse vs. Human IL-3 Systems
Key Research Findings
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Structural Biology: The IL-3Rα N-terminal domain (NTD) dynamically regulates cytokine binding and protects against aberrant signaling .
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Dual Receptor System: Mice employ two β-subunits (AIC2A/AIC2B) to form high-affinity IL-3 receptors, unlike humans .
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Therapeutic Implications: Targeting IL-3/CD123 axis shows potential in autoimmune diseases but risks hematologic toxicity .
Usage Guidelines
Product Specs
Product Science Overview
Interleukin-3 (Mouse Recombinant, sf9) is produced in Sf9 Baculovirus cells. The recombinant IL-3 is a single, glycosylated polypeptide chain containing 148 amino acids (27-166 a.a.) and has a molecular mass of 16.7 kDa. Under reducing conditions, it migrates at 18-28 kDa on SDS-PAGE . The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
The amino acid sequence of Interleukin-3 (Mouse Recombinant, sf9) is as follows:
ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG
EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT
SRPPQPASGS VSPNRGTVEC LEHHHHHH
