IP 10 Human, His
Description
Molecular Structure and Characteristics
IP-10 Human, His is a non-glycosylated polypeptide chain containing 77 amino acids (Val22–Pro98) with a molecular mass of 8.5–9 kDa . The His tag facilitates affinity chromatography purification, achieving >95% purity via SDS-PAGE and HPLC . Key features include:
Production and Purification
IP-10 Human, His is expressed in E. coli and purified via proprietary chromatographic methods . The His tag enables efficient immobilized metal affinity chromatography (IMAC), ensuring high yield and purity.
Key Production Steps:
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DNA Sequence Optimization: Human IP-10 mature chain (amino acids 22–98) is cloned into an expression vector.
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E. coli Expression: Induced under controlled conditions to maximize yield.
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Purification: IMAC followed by size-exclusion chromatography (SEC) or reverse-phase HPLC .
Functional Activity and Chemoattractant Properties
IP-10 Human, His binds to CXCR3, a receptor expressed on activated T cells, NK cells, and dendritic cells . Its activity includes:
Research Applications and Findings
IP-10 Human, His is used to study immune responses, viral infections, and cancer.
Immunological Roles
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CXCR3+ Cell Recruitment: IP-10 induces migration of activated T cells, NK cells, and monocytes, critical in Th1-mediated immunity .
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Viral Infections:
Cancer and Immunomodulation
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Pancreatic Cancer: IP-10 produced by stromal cells recruits CXCR3+ regulatory T cells (Tregs), promoting immunosuppression and tumor progression .
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Tumor Microenvironment: High IP-10 levels correlate with poor survival in pancreatic adenocarcinoma, indicating a dual role in antitumor vs. immunosuppressive effects .
Comparative Analysis of Recombinant IP-10 Variants
| Feature | IP-10 Human, His (E. coli) | Native IP-10 (Human) |
|---|---|---|
| Molecular Mass | 8.5–9 kDa | ~9 kDa |
| Post-Translational Modifications | None (non-glycosylated) | Native glycosylation |
| Purity | ≥95% (SDS-PAGE) | Variable (cell culture) |
| Stability | Lyophilized, pH 7.0 | Shorter half-life in serum |
| Source | E. coli | Human cells/tissues |
Limitations and Considerations
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Endotoxin Sensitivity: Despite low endotoxin levels (<0.1 EU/µg), verify compatibility with sensitive cell types .
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Species-Specific Activity: Human IP-10 may not cross-react with murine systems; confirm receptor compatibility .
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Proteolytic Stability: IP-10 is resistant to proteases but may degrade in acidic or oxidative environments .
Product Specs
Product Science Overview
IP-10 is an 8.7 kDa protein that is secreted by several cell types in response to interferon-gamma (IFN-γ). The recombinant form of IP-10, often tagged with a histidine (His) tag for purification purposes, is produced in various expression systems, including Escherichia coli . The His tag facilitates the purification of the protein using nickel affinity chromatography, ensuring a high degree of purity .
IP-10 plays a crucial role in the immune response. It acts as a chemoattractant for monocytes/macrophages, T cells, natural killer (NK) cells, and dendritic cells . Additionally, it promotes T cell adhesion to endothelial cells, exhibits antitumor activity, and inhibits bone marrow colony formation and angiogenesis .
Recombinant IP-10 is widely used in research to study its role in various biological processes and diseases. For instance, it is used in chemotaxis assays to investigate its ability to induce cell migration . Additionally, IP-10 is employed in binding assays to study its interaction with other proteins, such as Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) .
