CD40 Mouse

CD40 Mouse Recombinant
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Cat. No.
BT29603
Source
Sf9, Baculovirus cells.
Synonyms
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Key Antibodies

CloneIsotypeApplicationsSource
FGK4.5Rat IgG2a- In vivo CD40 activation
- B cell stimulation		Bio X Cell
1C10Rat IgG2aNeutralization studiesR&D Systems
3/23-Detects CD40 on dendritic cellsPMC

The FGK4.5 clone demonstrates agonistic activity, enhancing APC activation and anti-tumor responses . Neutralizing antibodies like 1C10 inhibit CD40-CD40L interactions .

CD40-Deficient Mouse Models

Genetic deletion models reveal CD40's roles in:

  • Atherosclerosis: T cell-specific CD40L deletion reduces plaque formation by 42% (p<0.01)

  • Viral immunity: CD40-/- mice show:

    • 3.8x higher influenza A titers at day 3 post-infection

    • 60% mortality vs 20% in WT with rVSV-EBOV challenge

  • Tumor immunology: CD40 stimulation on renal carcinoma cells:

    • Induces GM-CSF (12-fold increase) and MCP-1 (9-fold increase)

    • Reduces pulmonary metastases by 78% in SCID models

Immune Cell Regulation

Cell TypeCD40 FunctionConsequence of Activation
B cellsClass switching, germinal center formation IgG2a dominance (IgG1/IgG2a ratio = 0.2)
Dendritic cellsMaturation, IL-12 production 3.5x increase in MHC-II+CD11c+ cells
MacrophagesIFN-γ-dependent antiviral response 2.4x reduction in viral titers

Signaling Pathways

CD40 engagement triggers:

  1. TNFR-associated factor (TRAF) recruitment

  2. NF-κB and MAPK pathway activation

  3. IL-12/IFN-γ axis in macrophage-T cell crosstalk

Comparative Species Analysis

Humanized CD40 models show conserved expression patterns but distinct therapeutic responses:

  • Human CD40 transgenics require FcγRIIB engagement for antibody efficacy

  • Murine CD40 has extended intracellular domain (90aa vs human 62aa)

Product Specs

Introduction
CD40, a member of the TNF receptor superfamily, plays a crucial role in immune responses and inflammation. It is essential for various immune processes such as T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The transcription factor AKNA regulates the expression of both CD40 and its ligand, highlighting their importance in cell-to-cell interactions. Notably, CD40 interacts with the adaptor protein TNFR2, which mediates downstream signaling. The CD40-ligand interaction has been implicated in amyloid-beta-induced microglial activation, suggesting its involvement in the early stages of Alzheimer's disease.
Description
Recombinant CD40, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain with 180 amino acids (20-193 a.a.) and a molecular weight of 20.1 kDa. Under reducing conditions, it migrates at 18-28 kDa on SDS-PAGE. The protein contains a 6-amino acid His tag at the C-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CD40 protein solution is provided at a concentration of 1.0 mg/ml in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein stability.
Purity
The purity of CD40 is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CD40.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
LGQCVTCSDK QYLHDGQCCD LCQPGSRLTS HCTALEKTQC HPCDSGEFSA QWNREIRCHQ HRHCEPNQGL RVKKEGTAES DTVCTCKEGQ HCTSKDCEAC AQHTPCIPGF GVMEMATETT DTVCHPCPVG FFSNQSSLFE KCYPWTSCED KNLEVLQKGT SQTNVICGLK SRMRHHHHHH.

Product Science Overview

Structure and Function

CD40 is composed of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to CD40L, which is expressed on activated T cells and platelets . The interaction between CD40 and CD40L plays a crucial role in the activation of both innate and adaptive immune responses .

The CD40/CD40L interaction is essential for various immune functions, including:

  • B cell activation and proliferation: This interaction promotes the differentiation of B cells into plasma cells and memory B cells, leading to the production of antibodies .
  • Dendritic cell maturation: CD40 signaling enhances the ability of dendritic cells to present antigens and activate T cells .
  • Cytokine production: The interaction induces the production of various cytokines, which are critical for immune regulation .
Recombinant CD40 (Mouse)

Recombinant CD40 (Mouse) is a laboratory-produced version of the mouse CD40 protein. It is typically expressed in Chinese Hamster Ovary (CHO) cells to ensure proper folding and post-translational modifications . The recombinant protein is used in various research applications to study the CD40/CD40L interaction and its effects on immune responses.

Applications

Recombinant CD40 (Mouse) is widely used in immunological research to:

  • Investigate immune cell interactions: Researchers use recombinant CD40 to study how CD40/CD40L interactions influence the behavior of immune cells .
  • Develop immunotherapies: Understanding the CD40/CD40L pathway can lead to the development of novel immunotherapies for diseases such as cancer and autoimmune disorders .
  • Study disease models: Recombinant CD40 is used in animal models to investigate the role of CD40 in various diseases, including infections, inflammatory conditions, and tumors .

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