Product List

CD40 Human

CD40 Human Recombinant

CD40 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (21-193a.a) and having a molecular mass of 21.6kDa.
CD40 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29517
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CD40 Mouse

CD40 Mouse Recombinant

CD40 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 180 amino acids (20-193 a.a.) and having a molecular mass of 20.1kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).
CD40 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29603
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

CD40, also known as Cluster of Differentiation 40, is a type I transmembrane protein that belongs to the TNF receptor superfamily . It is primarily found on the surface of antigen-presenting cells (APCs) such as B cells, dendritic cells, and macrophages . CD40 plays a crucial role in the activation and regulation of immune responses .

Biological Properties

Key Biological Properties: CD40 is essential for various immune and inflammatory responses . It is involved in T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation .

Expression Patterns: CD40 is expressed on a variety of cells, including B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and several types of tumor cells .

Tissue Distribution: CD40 is widely distributed across different tissues, particularly in immune-related organs such as the spleen, lymph nodes, and thymus .

Biological Functions

Primary Biological Functions: CD40 is pivotal in mediating immune responses. It facilitates the activation of APCs, leading to the production of cytokines and the enhancement of antigen presentation .

Role in Immune Responses: CD40-CD40L interaction is crucial for the activation of T cells and B cells, promoting antibody production and the formation of memory cells .

Pathogen Recognition: CD40 plays a role in recognizing and responding to pathogens by activating APCs and initiating adaptive immune responses .

Modes of Action

Mechanisms with Other Molecules and Cells: CD40 interacts with its ligand, CD40L (CD154), which is primarily expressed on activated T cells . This interaction triggers a cascade of signaling events that lead to the activation of various immune cells .

Binding Partners: CD40 binds to CD40L, initiating signaling pathways that involve TRAF proteins and other adaptor molecules .

Downstream Signaling Cascades: The binding of CD40 to CD40L activates several downstream signaling pathways, including the NF-κB, MAPK, and PI3K pathways . These pathways are involved in the regulation of immune responses and inflammation .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of CD40 are tightly regulated at both the transcriptional and post-translational levels . Transcription factors such as NF-κB and AP-1 play a role in the transcriptional regulation of CD40 .

Transcriptional Regulation: CD40 expression is regulated by various transcription factors that bind to its promoter region .

Post-Translational Modifications: CD40 undergoes post-translational modifications such as phosphorylation, which can modulate its activity and signaling capabilities .

Applications

Biomedical Research: CD40 is extensively studied in the context of immune responses, autoimmune diseases, and cancer . It serves as a target for immunotherapy and vaccine development .

Diagnostic Tools: CD40 and its ligand are used as biomarkers for various diseases, including autoimmune disorders and cancers .

Therapeutic Strategies: Targeting the CD40-CD40L interaction has therapeutic potential in treating autoimmune diseases, cancers, and chronic inflammatory conditions .

Role in the Life Cycle

Development: CD40 is involved in the development and maturation of the immune system . It plays a role in the formation of germinal centers and the generation of memory B cells .

Aging: The expression and function of CD40 may change with age, potentially impacting immune responses in older individuals .

Disease: Dysregulation of CD40 signaling is associated with various diseases, including autoimmune disorders, cancers, and chronic inflammatory conditions .

© Copyright 2024 Thebiotek. All Rights Reserved.