FCGR3B Human

CD16b Human Recombinant

Recombinant human FCGR3B, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain containing 188 amino acids (21-200). It has a molecular mass of 21.4 kDa (observed molecular weight on SDS-PAGE will appear at approximately 28-40 kDa due to glycosylation). FCGR3B is expressed with an 8 amino acid His tag at the C-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT11
Source
Sf9, Baculovirus cells.
Appearance
Sterile filtered colorless solution.

FCGR2A Human

CD32a Human Recombinant

This product is a laboratory-produced version of the human FCGR2A protein. It is made in E. coli bacteria and consists of a single chain of 208 amino acids. The protein has a molecular weight of 23.0 kDa and includes a His-tag for purification purposes. The protein is purified to a high degree using specialized techniques.
Shipped with Ice Packs
Cat. No.
BT30939
Source
E.coli.
Appearance
Clear and colorless solution that has been sterilized by filtration.

FCGR2A Human, HEK

CD32a Human Recombinant, HEK

Recombinant human FCGR2A is a glycosylated polypeptide chain encompassing amino acids 30 to 218, resulting in a total of 195 amino acids. Its molecular mass is 21.6 kDa. For purification purposes, a 6-amino acid His-tag is fused to the C-terminus, and proprietary chromatographic techniques are employed.

Shipped with Ice Packs
Cat. No.
BT30954
Source

HEK293 Cells.

Appearance
A clear and sterile solution.

FCGR2B Human

CD32 Human Recombinant

This product consists of the extracellular domain of human FCGR2B, a protein encoded by the FCGR2B gene. It is produced in E. coli bacteria and purified to a high degree. The protein is a single polypeptide chain that lacks glycosylation modifications. It encompasses amino acids 46 to 217 of the mature FCGR2B protein and has a molecular weight of 22.0 kDa. For purification and detection purposes, a 25-amino acid Histidine tag is added to the N-terminus of the protein.
Shipped with Ice Packs
Cat. No.
BT30974
Source
Escherichia Coli.
Appearance
The product is a clear solution that has been sterilized by filtration.

FCGR3A Human

CD16a Human Recombinant

Recombinant human FCGR3A protein was produced in E. coli and purified to a high degree. It is a single, non-glycosylated polypeptide chain that contains 228 amino acids (including a 37 amino acid His-tag at the N-terminus) and has a molecular mass of 26 kDa. The protein sequence spans amino acids 18-208 of the mature FCGR3A protein.
Shipped with Ice Packs
Cat. No.
BT30980
Source
E.coli.
Appearance
Clear and colorless solution after sterilization by filtration.

FCGR3A Human, HEK

CD16a Human Recombinant, HEK

Recombinant human FCGR3A, produced in HEK293 cells, is a single polypeptide chain consisting of 197 amino acids (residues 18-208). With a molecular weight of 22.6 kDa, the protein contains a 6-amino acid His-tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT30986
Source

HEK293 Cells

Appearance
A clear, colorless solution that has been sterilized by filtration.

FCGR3A Human, Sf9

CD16a Human Recombinant, Sf9

Recombinant FCGR3A protein, derived from humans and produced in Sf9 insect cells (Baculovirus expression system), is a single, glycosylated polypeptide chain. It comprises 200 amino acids (specifically, amino acids 18-208) and exhibits a molecular mass of 22.8 kDa. When analyzed using SDS-PAGE, the apparent molecular size falls within the range of 28-40 kDa due to glycosylation. The protein includes a 6 amino acid Histidine tag (His tag) fused at the C-terminus to facilitate purification, which is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT30990
Source
Sf9, Baculovirus cells.
Appearance
A clear and colorless solution that has been sterilized by filtration.
Definition and Classification

The Fc fragment of IgG receptor, also known as FcγR, is a protein found on the surface of certain immune cells. It binds to the Fc region of IgG antibodies, facilitating various immune responses. FcγRs are classified into three main groups based on their structure and function: FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16). Each group has subtypes, such as FcγRIIa, FcγRIIb, and FcγRIIIa .

Biological Properties

Key Biological Properties: FcγRs are glycoproteins that play a crucial role in the immune system. They are involved in antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and the release of inflammatory mediators .

Expression Patterns and Tissue Distribution: FcγRs are expressed on various immune cells, including macrophages, neutrophils, dendritic cells, and natural killer (NK) cells. Their expression can be constitutive or inducible, depending on the cell type and the presence of inflammatory signals .

Biological Functions

Primary Biological Functions: FcγRs mediate the clearance of pathogens and infected cells by binding to the Fc region of IgG antibodies. This interaction triggers processes such as phagocytosis, ADCC, and the release of cytokines .

Role in Immune Responses and Pathogen Recognition: FcγRs play a vital role in the immune response by recognizing and binding to immune complexes. This binding activates immune cells to destroy pathogens and infected cells, enhancing the body’s ability to fight infections .

Modes of Action

Mechanisms with Other Molecules and Cells: FcγRs interact with IgG antibodies that are bound to antigens. This interaction leads to the cross-linking of FcγRs on the surface of immune cells, triggering intracellular signaling cascades .

Binding Partners and Downstream Signaling Cascades: FcγRs bind to the Fc region of IgG antibodies, leading to the activation of signaling pathways involving immunoreceptor tyrosine-based activation motifs (ITAMs). This activation results in various immune responses, including phagocytosis and cytokine release .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of FcγRs are regulated by various factors, including cytokines, transcription factors, and post-translational modifications .

Transcriptional Regulation and Post-Translational Modifications: FcγRs are regulated at the transcriptional level by cytokines and other signaling molecules. Post-translational modifications, such as glycosylation, also play a crucial role in modulating the activity and function of FcγRs .

Applications

Biomedical Research: FcγRs are used in research to study immune responses and develop therapeutic antibodies .

Diagnostic Tools: FcγRs are utilized in diagnostic assays to detect immune complexes and study antibody responses .

Therapeutic Strategies: FcγRs are targeted in various therapeutic strategies, including the development of monoclonal antibodies for cancer treatment and autoimmune diseases .

Role in the Life Cycle

Role Throughout the Life Cycle: FcγRs play a role in immune responses throughout the life cycle, from development to aging. They are involved in the clearance of pathogens, regulation of immune responses, and maintenance of immune homeostasis .

From Development to Aging and Disease: FcγRs are essential for the proper functioning of the immune system during development and aging. Dysregulation of FcγR activity can lead to immune-related diseases, such as autoimmune disorders and chronic inflammation .

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