CD14 Mouse

CD14 Mouse Recombinant

Recombinant mouse CD14 is produced using CHO cells transfected with mouse CD14 cDNA and cultured in a serum-free medium. The production process involves PCR amplification and cloning of the full-length mouse CD14 cDNA into the p-POL-DHFR expression vector. Purification of the recombinant protein is achieved through His-tag affinity chromatography, and its quality is verified using SDS-PAGE analysis. As the primary receptor for bacterial LPS, CD14 plays a crucial role in the myeloid differentiation process. The recombinant wild-type CD14 primarily exists as a 50 kDa protein.
Shipped with Ice Packs
Cat. No.
BT25705
Source
CHO-cells.
Appearance
White, lyophilized powder, sterile-filtered.

CD14 Mouse, Sf9

CD14 Mouse Recombinant, Sf9

Recombinant CD14, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. It consists of 357 amino acids (residues 16-366), resulting in a molecular weight of 38.3 kDa. This protein features a 6-amino acid His tag at the C-terminus to facilitate purification, which is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT25791
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless solution that has been sterilized by filtration.

CD14 Human

CD14 Human Recombinant

Recombinant human CD14, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 353 amino acids (residues 20-349). It has a molecular weight of 37.9 kDa. The protein includes a 23-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT25442
Source
E.coli.
Appearance
A clear, colorless solution that is sterile-filtered.

CD14 Human HEK

CD14 Human Recombinant HEK

Recombinant human CD14, produced in a mammalian expression system using human cells, is a single polypeptide chain consisting of 341 amino acids (20-352). It includes an 8 amino acid His-tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25520
Source
HEK293 cells.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.

CD14 Human, CHO

CD14 Human Recombinant, CHO

Recombinant human CD14 is produced in CHO cells transfected with the full-length human CD14 cDNA, which was PCR amplified and cloned into the p-POL-DHFR expression vector. As the primary receptor for bacterial LPS, CD14 plays a crucial role in myeloid differentiation. The predominant form of this recombinant wild-type CD14 is a 50 kDa protein comprising 335 amino acids and lacking the GPI anchor.
Shipped with Ice Packs
Cat. No.
BT25617
Source
CHO-cells.
Appearance
Sterile white lyophilized powder.
Definition and Classification

CD14, also known as Cluster of Differentiation 14, is a glycoprotein predominantly expressed on the surface of monocytes and macrophages. It plays a crucial role in the innate immune system by recognizing and binding to lipopolysaccharides (LPS) from Gram-negative bacteria . CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14) .

Biological Properties

Key Biological Properties: CD14 is a member of the leucine-rich repeat (LRR) family of proteins, which are involved in protein-protein interactions . It is a glycosylphosphatidylinositol (GPI)-anchored receptor .

Expression Patterns: CD14 is primarily expressed on monocytes, macrophages, and to a lesser extent, on neutrophils and dendritic cells . The soluble form of CD14 is secreted by the liver and monocytes .

Tissue Distribution: CD14 is found in various tissues, including the liver, spleen, and lymph nodes. It is also present in human milk, where it is believed to regulate microbial growth in the infant gut .

Biological Functions

Primary Biological Functions: CD14 acts as a co-receptor for the detection of bacterial LPS, working alongside Toll-like receptor 4 (TLR4) and MD-2 . It also recognizes other pathogen-associated molecular patterns (PAMPs) such as lipoteichoic acid from Gram-positive bacteria .

Role in Immune Responses: CD14 is essential for the initiation of immune responses. It binds to LPS and other PAMPs, facilitating their recognition by TLR4, which triggers downstream signaling pathways leading to the production of pro-inflammatory cytokines .

Pathogen Recognition: CD14 is involved in the recognition of a wide range of microbial products, including peptidoglycans and lipoteichoic acid .

Modes of Action

Mechanisms with Other Molecules and Cells: CD14 functions as a co-receptor with TLR4 and MD-2 for LPS detection . It can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) .

Binding Partners: CD14 interacts with LBP, TLR4, and MD-2 to form a complex that recognizes and responds to LPS .

Downstream Signaling Cascades: Upon LPS binding, CD14 facilitates the activation of the TLR4-TRAM-TRIF pathway, leading to the activation of transcription factors such as NF-κB and the production of pro-inflammatory cytokines .

Regulatory Mechanisms

Transcriptional Regulation: The expression of CD14 is regulated at the transcriptional level by various cytokines and transcription factors .

Post-Translational Modifications: CD14 undergoes post-translational modifications, including glycosylation, which are essential for its stability and function .

Applications

Biomedical Research: CD14 is widely used as a marker for monocytes and macrophages in immunological studies .

Diagnostic Tools: Soluble CD14 levels in the blood can serve as a biomarker for various inflammatory conditions and infections .

Therapeutic Strategies: Targeting CD14 and its signaling pathways holds potential for the development of therapies for inflammatory diseases and sepsis .

Role in the Life Cycle

Development: CD14 is involved in the differentiation of monocytes into macrophages and dendritic cells .

Aging and Disease: CD14 expression and function can be altered in various diseases, including sepsis, chronic inflammatory conditions, and metabolic disorders . Its role in immune responses makes it a critical player in the body’s defense mechanisms throughout life .

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