CD244 Human

CD244 Human Recombinant

Recombinant human CD244, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 230 amino acids (residues 19-224). It has a molecular weight of 25.5 kDa. The protein includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26954
Source
E.coli.
Appearance
Clear, colorless solution, sterile-filtered.

CD244 Human, Sf9

CD244 Human Recombinant, Sf9

This product consists of the extracellular domain of human CD244, recombinantly produced in Sf9 insect cells. The protein is a single, glycosylated polypeptide chain comprising 448 amino acids (specifically, residues 19 to 224a.a.) with a molecular weight of 50.2 kDa. It is important to note that due to glycosylation, the protein might appear between 50-70 kDa on SDS-PAGE. For purification and detection purposes, a 239 amino acid hIgG-His tag is added at the C-terminus. The protein is purified using proprietary chromatographic techniques to ensure its high purity and quality.
Shipped with Ice Packs
Cat. No.
BT27043
Source

Sf9, Insect cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

CD244, also known as 2B4 or SLAMF4, is a type-I transmembrane protein that belongs to the signaling lymphocytic activation molecule (SLAM) family of receptors. It is encoded by the CD244 gene located on the long arm of human chromosome 1 . CD244 is a member of the CD2 subset of the immunoglobulin (Ig) superfamily .

Biological Properties

Key Biological Properties: CD244 is a cell surface receptor expressed on natural killer (NK) cells, T cells, monocytes, eosinophils, myeloid-derived suppressor cells, and dendritic cells . It primarily binds to its ligand CD48, another member of the SLAM family .

Expression Patterns and Tissue Distribution: CD244 is expressed in various hematopoietic cells, including NK cells, T cells, monocytes, basophils, eosinophils, mast cells, and dendritic cells . It is highly expressed in granulocytes, monocytes, blood, testicles, spleen, bone marrow, lymph nodes, and the liver .

Biological Functions

Primary Biological Functions: CD244 plays a crucial role in the regulation of the immune system. It provides stimulatory or inhibitory signals that regulate various immune responses, such as cytotoxicity, cytokine production, and intercellular interactions .

Role in Immune Responses and Pathogen Recognition: CD244 is involved in the activation and differentiation of a wide variety of immune cells, thus playing a significant role in both innate and adaptive immune responses . It is essential for the expansion and activation of NK cells and the regulation of immune responses in CD8+ T cells .

Modes of Action

Mechanisms with Other Molecules and Cells: CD244 primarily binds to CD48 on adjacent cells, transmitting stimulatory or inhibitory signals that regulate immune function . It can also bind to other low-affinity ligands such as CD229 .

Binding Partners and Downstream Signaling Cascades: The interaction between CD244 and CD48 involves both trans and cis interactions. The intracellular domain of CD244 contains immunoreceptor tyrosine-based switch motifs (ITSMs) that interact with SH2 domain-containing proteins, such as SAP and EAT-2, which are involved in downstream signaling .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CD244 are regulated by various mechanisms, including transcriptional regulation and post-translational modifications . The presence or absence of small cytoplasmic adapter proteins, such as SH2D1A/SAP and SH2D1B/EAT-2, also controls its activities .

Transcriptional Regulation and Post-Translational Modifications: CD244 expression is regulated at the transcriptional level, and its activity can be modulated by post-translational modifications, such as phosphorylation .

Applications

Biomedical Research: CD244 is a valuable target in biomedical research due to its role in immune regulation and its involvement in various immune-related diseases, such as autoimmune diseases, infectious diseases, and cancers .

Diagnostic Tools and Therapeutic Strategies: CD244 can be used as a diagnostic and prognostic marker for various diseases. It also holds potential as a therapeutic target for immunotherapy, particularly in the treatment of tumors .

Role in the Life Cycle

Role Throughout the Life Cycle: CD244 plays a role throughout the life cycle, from development to aging and disease. It is involved in the regulation of immune responses during development and continues to play a crucial role in maintaining immune homeostasis throughout life .

From Development to Aging and Disease: Abnormal interactions between immune cells involving CD244 contribute to the onset and progression of autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and type 1 diabetes (T1D) .

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