Product List

CD200 Human, Sf9

CD200 Human Recombinant, sf9

CD200 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 444 amino acids (31-232a.a.) and having a molecular mass of 49.7kDa (Molecular size on SDS-PAGE will appear at approximately 57-70 kDa).
CD200 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT26127
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

CD200 Human

CD200 Human Recombinant

CD200 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (31-232 a.a) and having a molecular mass of 24.8kDa.
CD200 is fused to a 23 amino acid His-tag at N-terminus.
Shipped with Ice Packs
Cat. No.
BT26217
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

CD200 Mouse

CD200 Mouse Recombinant

CD200 Mouse Recombinant produced in Baculovirus is a single, glycosylated, polypeptide chain containing 441 amino acids (31-232aa) and having a molecular mass of 49.5kDa.
CD200 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT26312
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

CD200R1 Human

CD200 Receptor 1 Human Recombinant

CD200R1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 462 amino acids (24-243 a.a.) and having a molecular mass of 51.9kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD200R1 is expressed with 242 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT26402
Source

Sf9, Baculovirus cells.

Appearance

Sterile filtered colorless solution.

CD200R1 Mouse

CD200 Receptor 1 Mouse Recombinant

CD200R1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 455 amino acids (26-238a.a.) and having a molecular mass of 50.4kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CD200R1 is expressed with a 239 amino acid hIgG His tag at C-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26472
Source
Sf9, Insect cells.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

CD200, also known as OX-2, is a type-1 membrane glycoprotein that belongs to the immunoglobulin superfamily (IgSF). It is encoded by the CD200 gene located on chromosome 3q13.2 in humans . CD200 is characterized by two extracellular immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail .

Biological Properties

Key Biological Properties: CD200 is involved in immunosuppressive signaling through its interaction with the CD200 receptor (CD200R) .

Expression Patterns and Tissue Distribution: CD200 is broadly expressed on various cell types, including B lymphocytes, a subset of T lymphocytes, dendritic cells, endothelial cells, and neurons . It is also found in tissues such as the frontal pole, hypothalamus, and aorta .

Biological Functions

Primary Biological Functions: CD200 plays a crucial role in regulating immune responses by delivering immunosuppressive signals through its receptor, CD200R . This interaction helps maintain immune homeostasis and promotes self-tolerance .

Role in Immune Responses and Pathogen Recognition: CD200 modulates immune cell activity by inhibiting proinflammatory cytokine secretion, enhancing anti-inflammatory cytokine secretion, and promoting the induction of regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs) . It also inhibits natural killer (NK) cell activation and cytotoxic T cell functions .

Modes of Action

Mechanisms with Other Molecules and Cells: CD200 interacts with its receptor, CD200R, which is expressed on monocytes, myeloid cells, and T lymphocytes . The engagement of CD200 with CD200R leads to tyrosine phosphorylation on the CD200R cytoplasmic PTB domain, recruiting adaptor proteins DOK-1 and DOK-2 .

Binding Partners and Downstream Signaling Cascades: The binding of CD200 to CD200R promotes the binding of SHIP to DOK-1 and the recruitment of RasGAP, which negatively regulates the MAPK/ERK signaling pathway . This signaling cascade results in the inhibition of immune responses and the promotion of immune tolerance .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of CD200 is regulated at the transcriptional level by C/EBP-β, which also regulates responses induced by IFN-γ, IL-6, IL-1, and TNF-α .

Transcriptional Regulation and Post-Translational Modifications: CD200 expression is modulated by various cytokines and transcription factors, ensuring its appropriate expression in different tissues and under different physiological conditions .

Applications

Biomedical Research: CD200 is used as a marker in the differential diagnosis of B-cell neoplasms and in monitoring minimal residual disease .

Diagnostic Tools: Anti-CD200 antibodies are employed in flow cytometry, immunohistochemistry, Western blot, ELISA, and immunocytochemistry for detecting CD200 expression .

Therapeutic Strategies: Targeting the CD200/CD200R axis has shown potential in cancer immunotherapy, particularly in treating hematologic malignancies and solid tumors . CD200 checkpoint reversal is being explored as a novel approach to immunotherapy .

Role in the Life Cycle

Role Throughout the Life Cycle: CD200 plays a role in various stages of life, from development to aging and disease. It contributes to immune regulation during development and helps maintain immune homeostasis in adulthood . In aging and disease, CD200 expression patterns can influence the progression of conditions such as chronic lymphocytic leukemia and other malignancies .

CD200’s role in enhancing synaptic plasticity and promoting functional recovery after stroke further highlights its importance in neurological health .

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