CD47 Human, IgG-His

CD47 Human Recombinant, IgG-His Tag

Recombinant human CD47, expressed in Sf9 insect cells, is a non-glycosylated polypeptide chain consisting of 362 amino acids (residues 19-141). It has a molecular weight of 40.9 kDa and appears between 40-57 kDa on SDS-PAGE under reducing conditions due to glycosylation. The protein is fused to a 239 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT29874
Source
Sf9, Insect cells.
Appearance
Clear, colorless liquid solution, sterile-filtered.

CD47 Human

CD47 Human Recombinant

Recombinant CD47 protein was expressed in Sf9 insect cells using a baculovirus expression system. The protein encompasses amino acids 19-141 of the human CD47 sequence, with a 6-amino acid Histidine tag added at the C-terminus for purification purposes. The resulting protein has a total of 129 amino acids and a molecular weight of 14.7 kDa. SDS-PAGE analysis under reducing conditions reveals multiple bands between 18-28 kDa, indicative of glycosylation. The protein was purified using proprietary chromatographic techniques to ensure high purity.
Shipped with Ice Packs
Cat. No.
BT29820
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless liquid, free from particulate matter
Definition and Classification

CD47, also known as Cluster of Differentiation 47 or integrin-associated protein (IAP), is a transmembrane protein encoded by the CD47 gene in humans . It belongs to the immunoglobulin superfamily and interacts with membrane integrins, thrombospondin-1 (TSP-1), and signal-regulatory protein alpha (SIRPα) .

Biological Properties

Key Biological Properties: CD47 is a 50 kDa glycoprotein with an extracellular N-terminal IgV domain, five transmembrane helices, and a C-terminal cytoplasmic tail . It has four spliced isoforms, with isoform 2 being the most abundantly expressed .

Expression Patterns and Tissue Distribution: CD47 is ubiquitously expressed on a variety of hematopoietic and non-hematopoietic cells . It is highly expressed in tissues such as the gingival epithelium, palpebral conjunctiva, and pancreatic ductal cells . Its expression levels vary depending on the cell type and physiological conditions .

Biological Functions

Primary Biological Functions: CD47 plays a crucial role in various cellular processes, including apoptosis, proliferation, adhesion, and migration . It acts as a “don’t eat me” signal to prevent phagocytosis by macrophages, thus maintaining immune homeostasis .

Role in Immune Responses and Pathogen Recognition: CD47 modulates immune responses by interacting with its ligands, including TSP-1, SIRPα, and integrins . It regulates the activation of dendritic cells, T cells, and B cells, and is involved in the transmigration of neutrophils .

Modes of Action

Mechanisms with Other Molecules and Cells: CD47 interacts with SIRPα on macrophages, sending a “don’t eat me” signal that inhibits phagocytosis . It also binds to TSP-1 and integrins, modulating cellular adhesion and migration .

Binding Partners and Downstream Signaling Cascades: CD47’s interaction with SIRPα leads to the activation of SHP-1 and SHP-2, which are protein tyrosine phosphatases involved in downstream signaling . This interaction regulates various cellular responses, including inhibition of phagocytosis and stimulation of cell-cell fusion .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: CD47 expression is regulated at both transcriptional and post-translational levels . Transcriptional regulation involves various transcription factors that bind to the CD47 promoter region . Post-translational modifications, such as glycosylation, also play a role in modulating CD47’s activity .

Transcriptional Regulation and Post-Translational Modifications: The cytoplasmic tail of CD47 undergoes phosphorylation, which influences its interaction with intracellular signaling molecules . Glycosylation of the extracellular domain affects its binding affinity to ligands .

Applications

Biomedical Research: CD47 is a prominent target in cancer therapy due to its role in immune evasion by tumor cells . Blocking CD47-SIRPα interaction enhances cancer cell clearance by macrophages .

Diagnostic Tools and Therapeutic Strategies: Anti-CD47 antibodies are being developed as therapeutic agents to promote phagocytosis of cancer cells . CD47 is also being explored as a biomarker for various diseases, including hematological malignancies .

Role in the Life Cycle

Role Throughout the Life Cycle: CD47 is involved in various stages of the life cycle, from development to aging . During development, it regulates cellular adhesion and migration . In aging, CD47 expression influences the clearance of aged cells by phagocytosis . Dysregulation of CD47 is associated with diseases such as cancer and neurodegenerative disorders .

CD47’s multifaceted roles in cellular processes and immune regulation make it a critical molecule in both health and disease. Its potential as a therapeutic target continues to drive research in various biomedical fields.

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