Product List

CD27 Human

CD27 Human Recombinant

CD27 Human Recombinant produced in E. coli is a single polypeptide chain containing 196 amino acids (21-191) and having a molecular mass of 21.8 kDa.
CD27 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27132
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

CD27 Human, HEK

CD27 Human Recombinant, HEK

CD27 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (20-191 a.a) containing a total of 411 amino acids, having a molecular mass of 46.2kDa.
CD27 is fused to a 239 amino acid hIgG-His-Tag at C-terminus,and is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT27205
Source

HEK293 Cells.

Appearance

Sterile Filtered colorless solution.

CD27 Human, sf9

CD27 Human Recombinant, sf9

CD27 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413 amino acids (21-191a.a.) and having a molecular mass of 46.4kDa (Molecular size on SDS-PAGE will appear at approximately 28-57kDa).
CD27 is fused with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27281
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

CD27 is a member of the tumor necrosis factor receptor (TNFR) superfamily, specifically known as TNFRSF7 . It is a type I transmembrane protein that plays a crucial role in the regulation of immune responses .

Biological Properties

Key Biological Properties: CD27 is essential for the generation and long-term maintenance of T cell immunity . It binds to its ligand, CD70, and is involved in the regulation of B-cell activation and immunoglobulin synthesis .

Expression Patterns: CD27 is expressed on both naïve and activated effector T cells, as well as on NK cells and activated B cells . During mouse embryonic development, CD27 is expressed in the aorta-gonad-mesonephros region, defining the first adult definitive hematopoietic stem cells .

Tissue Distribution: CD27 is predominantly found in lymphoid tissues such as the lymph nodes, spleen, and thymus . It is also expressed in the bone marrow, blood, and various other tissues .

Biological Functions

Primary Biological Functions: CD27 is crucial for the generation and maintenance of T cell immunity . It plays a significant role in the regulation of B-cell activation and immunoglobulin synthesis .

Role in Immune Responses: CD27 is involved in the differentiation and clonal expansion of T cells upon binding to CD70 . This interaction enhances the survival and memory of cytotoxic T cells and increases the production of certain cytokines .

Pathogen Recognition: CD27, through its role in T and B cell activation, indirectly contributes to the recognition and response to pathogens .

Modes of Action

Mechanisms with Other Molecules and Cells: CD27 interacts with its ligand CD70, leading to a signaling cascade that results in T cell differentiation and clonal expansion . This cascade also involves the activation of NF-κB and MAPK8/JNK pathways .

Binding Partners: The primary binding partner of CD27 is CD70 . This interaction is crucial for its role in immune regulation.

Downstream Signaling Cascades: Upon binding to CD70, CD27 transduces signals that activate NF-κB and MAPK8/JNK pathways, leading to various immune responses .

Regulatory Mechanisms

Regulatory Mechanisms: The expression of CD27 is regulated at multiple levels. T cell activation via the TCR/CD3 complex or the CD2 molecule significantly increases CD27 expression . Conversely, direct stimulation of protein kinase C by phorbol esters down-regulates CD27 surface expression .

Transcriptional Regulation: Specific transcription factors involved in the regulation of CD27 expression are not well-documented, but it is known that T cell activation plays a significant role .

Post-Translational Modifications: Post-translational modifications of CD27, such as phosphorylation, may play a role in its function and regulation, although detailed mechanisms are not fully understood .

Applications

Biomedical Research: CD27 is used as a marker to distinguish different subsets of T cells, B cells, and NK cells . It is also a target for cancer immunotherapy due to its role in T cell activation .

Diagnostic Tools: CD27 expression levels can be used as a diagnostic marker for certain immune deficiencies and lymphoproliferative disorders .

Therapeutic Strategies: Monoclonal antibodies targeting CD27 are being explored for their potential in cancer immunotherapy . These therapies aim to enhance T cell responses against tumors by modulating CD27 signaling .

Role in the Life Cycle

Development: CD27 is expressed early in life and plays a role in the formation of memory B cells . It continues to be important throughout life for the maintenance of immune memory .

Aging and Disease: The expression and function of CD27 may decline with age, contributing to weakened immune responses in the elderly . Additionally, CD27 is involved in various immune-related diseases, including autoimmune disorders and cancers .

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