Product List

CD23 Human, Sf9

CD23 Human Recombinant, Sf9

CD23 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 283 amino acids (48-321a.a.) and having a molecular mass of 32.0kDa. 
CD23 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT27344
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

sCD23 Human

Soluble CD23 Human Recombinant

sCD23 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19.2kDa.
The sCD23 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27420
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Introduction

Definition and Classification

CD23, also known as Fc epsilon RII (FcεRII), is a low-affinity receptor for Immunoglobulin E (IgE), an antibody isotype involved in allergic reactions and resistance to parasites . CD23 is a C-type lectin and is classified into two forms: CD23a and CD23b. CD23a is present on follicular B cells, while CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages .

Biological Properties

Key Biological Properties: CD23 is a type II transmembrane glycoprotein with a molecular weight of approximately 45 kDa . It consists of a large C-terminal globular extracellular domain, a stalk region, a single hydrophobic membrane-spanning region, and a short N-terminal cytoplasmic domain .

Expression Patterns and Tissue Distribution: CD23 is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets . It is also found in various tissues, including the spleen, lymph nodes, and bone marrow .

Biological Functions

Primary Biological Functions: CD23 plays a crucial role in the regulation of IgE levels and the transportation of IgE immune complexes . It is involved in antibody feedback regulation, where antigens captured by IgE antibodies bind to CD23 molecules on B cells and are transported to the B cell follicles of the spleen .

Role in Immune Responses and Pathogen Recognition: CD23 is essential in modulating immune responses, particularly in allergic reactions. It helps regulate the synthesis of IgE, the antibody isotype that mediates allergic responses .

Modes of Action

Mechanisms with Other Molecules and Cells: CD23 interacts with IgE and CD21, regulating the synthesis of IgE . It can be cleaved from cell surfaces to yield soluble CD23 (sCD23) proteins that have cytokine-like activities .

Binding Partners and Downstream Signaling Cascades: CD23 binds to IgE and CD21, influencing various immune pathways. The activation of CD23 can result in the downregulation of IgE production or modulation of B cell activity, reducing inflammation and allergic responses .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: CD23 expression is regulated by several cytokines, including interleukin-4 (IL-4) and interferons . It can be cleaved from cell surfaces to yield soluble CD23 proteins, which have pleiotropic cytokine-like activities .

Transcriptional Regulation and Post-Translational Modifications: The gene encoding CD23 is located on chromosome 19 and is regulated by various stimuli . Post-translational modifications, such as glycosylation, play a role in its function and stability .

Applications

Biomedical Research: CD23 is used as a marker in the diagnosis of chronic lymphocytic leukemia (CLL) and other lymphoid leukemias . It is also a target for therapeutic intervention in allergic diseases and autoimmune conditions .

Diagnostic Tools and Therapeutic Strategies: CD23 agonists are being explored for their potential in treating allergic diseases such as asthma, allergic rhinitis, and atopic dermatitis . These agonists can modulate immune responses by targeting CD23, providing relief from symptoms and improving the quality of life for patients .

Role in the Life Cycle

Role Throughout the Life Cycle: CD23 plays a role in the development and growth of B cells. It is involved in the regulation of IgE levels and immune responses throughout the life cycle . CD23’s function in antibody feedback regulation and immune modulation is crucial from development to aging and disease .

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