Product List

CD58 Human

CD58 Human Recombinant

CD58 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (29-215 a.a) and having a molecular mass of 23.8kDa.
CD58 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques
Shipped with Ice Packs
Cat. No.
BT30612
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

CD58 Human, Sf9

CD58 Human Recombinant, Sf9

CD58 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 196 amino acids (29-215a.a.) and having a molecular mass of 22.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
CD58 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT30639
Source

Sf9, Baculovirus cells.

Appearance

Sterile filtered colorless solution.

Introduction

Definition and Classification

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule that plays a crucial role in the immune response. It is a heavily glycosylated surface glycoprotein with a molecular weight ranging from 40 to 70 kDa . CD58 is classified as a member of the immunoglobulin superfamily and is expressed on various cell types, including antigen-presenting cells (APCs), macrophages, and other tissue cells .

Biological Properties

Key Biological Properties: CD58 is a costimulatory receptor that interacts with its natural ligand, CD2, primarily found on T cells and natural killer (NK) cells . This interaction is essential for the activation and proliferation of these immune cells.

Expression Patterns and Tissue Distribution: CD58 is extensively expressed on both hematopoietic and non-hematopoietic cells . It is found on the surface of APCs, including dendritic cells and macrophages, as well as on various other tissue cells . Additionally, a soluble form of CD58 (sCD58) is present in cellular supernatant and local tissues .

Biological Functions

Primary Biological Functions: CD58 plays a vital role in immune responses by facilitating cell adhesion and recognition. The CD2-CD58 interaction is a key component of the immunological synapse, which induces the activation and proliferation of T and NK cells . This interaction also triggers a series of intracellular signaling events in both T/NK cells and target cells, promoting cell adhesion and recognition .

Role in Immune Responses and Pathogen Recognition: CD58 is involved in various immune regulatory responses, including antiviral responses, inflammation in autoimmune diseases, and immune rejection in organ transplants . It also plays a role in the immune evasion of tumor cells .

Modes of Action

Mechanisms with Other Molecules and Cells: CD58 binds to CD2 (LFA-2) on T cells, strengthening the adhesion and recognition between T cells and APCs . This binding facilitates signal transduction necessary for an immune response .

Binding Partners and Downstream Signaling Cascades: The CD2-CD58 interaction is crucial for the formation of a co-stimulatory signal, contributing to the proliferation and regulation of T cell responses via signaling transduction . This interaction also activates the AKT/GSK-3β/β-catenin signaling pathway, promoting the expression of Wnt/β-catenin target proteins and TCF/LEF-mediated transcriptional activity .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: CD58 expression is regulated by cytokines in a cell-dependent manner . Additionally, polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis, and certain genetic variations can enhance CD58 promoter activity .

Transcriptional Regulation and Post-Translational Modifications: The expression of CD58 is influenced by transcription factors such as ASCL2, which is a target of the Wnt signaling pathway . Post-translational modifications, including glycosylation, are essential for the proper function of CD58 .

Applications

Biomedical Research: CD58 is a valuable marker in immunological studies due to its role in cell adhesion and immune responses .

Diagnostic Tools: CD58 expression levels can be used as a diagnostic marker for various diseases, including multiple sclerosis and certain types of cancer .

Therapeutic Strategies: Targeting the CD2-CD58 interaction has potential therapeutic applications in immunotherapy, particularly in enhancing anti-tumor responses and regulating immune responses in autoimmune diseases .

Role in the Life Cycle

Role Throughout the Life Cycle: CD58 plays a role in immune responses throughout the life cycle, from development to aging. It is involved in the activation and proliferation of T and NK cells, which are crucial for immune defense . In aging individuals, the CD58/CD2 interaction remains important for maintaining immune function, particularly in the context of chronic infections and immune senescence .

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