Monoclonal Antibodies

This monoclonal antibody against human ICAM1 is generated using a unique process involving immunization of mice with a synthesized peptide derived from the human CD54 protein. After immunization, B cells are isolated from the splenocytes of these mice and fused with myeloma cells to create hybridoma cells. These hybridomas are screened for production of the desired ICAM1 antibody. Once identified, the hybridoma cells are cultured in the mouse abdominal cavity to produce ascites fluid containing the antibody. The ICAM1 monoclonal antibody is then purified from the ascites fluid through affinity chromatography using a specific immunogen. This purified antibody is highly specific for human ICAM1 and suitable for applications such as ELISA and immunohistochemistry (IHC).

ICAM1, also known as CD54, is a cell surface glycoprotein that plays a critical role in various biological processes, particularly in immune responses. As a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2), ICAM1 facilitates leukocyte trans-endothelial migration by promoting the assembly of endothelial apical cups. This process is mediated by the activation of ARHGEF26/SGEF and RHOG. ICAM1 also interacts with other leukocyte integrins, such as Mac-1, contributing to leukocyte trafficking, antigen presentation, and T-cell activation.

Beyond its role in immune responses, ICAM1 has been implicated in the pathogenesis of various diseases, including inflammatory and autoimmune diseases, atherosclerosis, and cancer. Its involvement in these conditions highlights the importance of ICAM1 in cell-cell interactions and inflammatory processes.

This monoclonal antibody targeting human CD99 protein is generated through a rigorous process. Briefly, the synthesized peptide derived from human CD99 protein is used to immunize mice, stimulating the production of specific antibodies. Mouse B cells producing these antibodies are then fused with myeloma cells to create hybridomas. Antibody-secreting hybridomas are meticulously selected and cultured in the mouse abdominal cavity. Finally, the CD99 monoclonal antibody is purified from mouse ascites by affinity chromatography using the specific immunogen. This antibody is suitable for recognizing human CD99 protein in ELISA and IHC applications.

CD99 protein, also known as MIC2 or E2 antigen, plays crucial roles in various cellular processes, including adhesion, apoptosis, differentiation, and migration. Within lymphocytes, CD99 significantly contributes to T cell development and maturation, as well as T cell activation and signaling. Additionally, it has been demonstrated to regulate the adhesion and migration of lymphocytes and monocytes. Moreover, CD99 has been implicated in the regulation of apoptosis and is suggested to play a role in the pathogenesis of specific cancers, including Ewing's sarcoma and leukemia.